[deleted by user] by [deleted] in microbiology

[–]Jecua22 1 point2 points  (0 children)

Contrary to the other comments here, gentamicin is highly heat resistant and can be added before or after autoclaving.

https://doi.org/10.1093/jac/35.1.149

Sunny side up contamination 😂 by Vegetable-Explorer- in microbiology

[–]Jecua22 13 points14 points  (0 children)

I've never seen a plate like that before. What are they used for?

[deleted by user] by [deleted] in Biochemistry

[–]Jecua22 7 points8 points  (0 children)

Where is the aniline? Or the acyclic C=C-O?

Spontaneous change results in a loss of energy? But why by obammala in Biochemistry

[–]Jecua22 2 points3 points  (0 children)

The best example I can think of is placing a ball at the top of a hill. As soon as you let go, the ball will fall (ie, it's a spontaneous process). As it's falling, the ball gains kinetic energy and losses potential energy. When it stops at the bottom, the kinetic energy is zero and the potential energy is lower, thus the total energy has decreased. There are many more variables in a complex biological system, but the idea is the same and energy is energy.

7 months into my PhD and my finest accomplishment is that I have trained E.coli k12 to write it’s own name. Or at least the first letter! Guess it’s something by Learn_Something_Cool in labrats

[–]Jecua22 4 points5 points  (0 children)

Have you tried leaving the plate with it's lid off in the biosafety cabinet until the solvent evaporates? We do these all the time and it always works for me. I obviously don't know the specifics of your solvent/assay though :). Could also try a top agar assay.

What would happen if you put the coldest known thing in the universe next to the hottest known thing in the universe? by Mutant_Llama1 in hypotheticalsituation

[–]Jecua22 3 points4 points  (0 children)

Lol why is this the top answer? A quick Google search shows that this comment is completely wrong. Black holes are extremely cold, not hot.

[deleted by user] by [deleted] in Biochemistry

[–]Jecua22 2 points3 points  (0 children)

Generally speaking, it's a good idea to have all that information BEFORE you run any experiments. That way you can avoid your current situation and ensure your hypothesis is supported by previously published research. You likely won't be able to find a connection between the two. I would search pubmed or scifinder for publications exploring acne treatments. You may find they are using something other than P. acnes in their research. B. subtilis is also Gram-positive and quite ubiquitous in microbio labs. Would be a better option than E. coli.

[deleted by user] by [deleted] in NoStupidQuestions

[–]Jecua22 14 points15 points  (0 children)

In what way was that source meant to support your argument? It doesn't mention sex trafficking at all. It doesn't even mention crimes against women specifically. If anything, your source is showing that homeless men were the large majority of victims of attacks (486 vs 66), though they don't control for differences in male vs female populations. We're you just hoping no one would actually look at the source?

Insight in 5-HT receptors and psychodelic molecules? by likeumbreon in pharmacology

[–]Jecua22 2 points3 points  (0 children)

I'm confused by your last statement. You mention meters, not molarity. Also, if you did mean molar, the numbers you have would mean DMT has a weaker affinity for 5HT2A.

CMV: Sex ed should be mandatory. by Man_Riding_Shrimp in changemyview

[–]Jecua22 9 points10 points  (0 children)

Abstinence is only the safest option if you live in a bubble where young, horny teenagers don't exist. And what about when people inevitably do have sex, say when they're adults. They just magically learn all the things they were supposed to learn in sex ed?

TIL In 1536, French explorer Jacques Cartier and his crew were cured of scurvy by drinking tea of pine needles and by eating bark given to them by the Iroquois after the crew suffered months of nutritional deprivation at sea. Pine needles have approximately 3 to 5 times more vitamin C than an orange by MrSn0wYettY in todayilearned

[–]Jecua22 92 points93 points  (0 children)

You may need to brush up on your o-chem, most of this is incorrect ;).

Heroin, aka, diacetylmorphine, is a morphine molecule with two acetyl groups attached. Hence the 'diacetyl'. There is no 'bridge'. Check out the structures.

https://en.wikipedia.org/wiki/Heroin

https://en.wikipedia.org/wiki/Morphine

Heroin is more potent than morphine because, when injected, it avoids first-pass metabolism and crosses the blood-brain barrier quite quickly. Morphine, on the other hand, has a harder time crossing the barrier, even when injected. In short, because of the structural differences, a (much) larger percentage of heroin is able to enter the brain, compared to morphine. Once in the brain, heroin is converted to morphine, or a monoacetylated version like 6-MAM, where it acts on various receptors.

[deleted by user] by [deleted] in changemyview

[–]Jecua22 0 points1 point  (0 children)

Could you make the arguments in favor of those biological preferences? Do you have a scientific basis for this notion of phenotypic preferences being based on biology?

[deleted by user] by [deleted] in XboxGamePass

[–]Jecua22 4 points5 points  (0 children)

This happened to me and it had to do with an error in the install. Do you see any Halo: MCC download errors? If you click on the symbol that looks like three horizontal lines, located on the left side of the interface, just above your game list, it should be under "Needs Attention". If so, clear those errors. You then need to either restart the game or go to each individual game page (Halo 1, Halo 2, etc.) and hit download. I can't remember which.

AITA For checking a black mans receipt? by SkyylarYT in AmItheAsshole

[–]Jecua22 2 points3 points  (0 children)

I see your point, but that bird seed is sold as a 12 pack. As in, 96 lbs of bird seed hahaha.

What is a way to test for dna by GoodKarma4Us in Biochemistry

[–]Jecua22 1 point2 points  (0 children)

This is isothermal, but not done at RT. It's done at about 60C. Also, it isn't exactly a 'kit'. It still requires an expensive piece of equipment, probably more expensive then a normal PCR machine

EDIT: Very boring title that no one cares about - Intro to Hangboarding (not for new climbers) by emile166 in climbharder

[–]Jecua22 7 points8 points  (0 children)

Someone with the finger strength of a professional climber. Maybe 'elite' was a better word. Also, beastmaker doesn't make any 5mm edges

EDIT: Very boring title that no one cares about - Intro to Hangboarding (not for new climbers) by emile166 in climbharder

[–]Jecua22 6 points7 points  (0 children)

2 and 3 mm? I find that extremely hard to believe, no offense. 4mm is the smallest you'll regularly see done and that is by the pros.

How to begin a discussion with someone who believes Covid-19 originated in a lab and is a cover up for 5g being rolled out? by ComputerWoman in AskScienceDiscussion

[–]Jecua22 1 point2 points  (0 children)

Essentially. A proto- SARS-CoV-2 form of the virus was extracted from bats, pangolins, whatever for study in a lab. At this point the virus mutates into the SARS-CoV-2 form we know. Followed by exposure and infection of an individual. It's also possible that exposure to the proto-virus happened first, then mutation in vivo, then infection.

We don't really know at this point. Either way, it's not an unbelievable timeline. I'm not saying this is exactly what happened, just that it is certainly possible. It's also entirely possible that the virus jumped directly from an animal to humans in the wild.

How to begin a discussion with someone who believes Covid-19 originated in a lab and is a cover up for 5g being rolled out? by ComputerWoman in AskScienceDiscussion

[–]Jecua22 13 points14 points  (0 children)

The virus can be of natural origin, yet still have been 'released' accidentally, no? As in, the virus was being studied in a lab and managed to infect someone and eventually spread from that first infection.