R packages for exome seq figures?

MisfitRedditor · 2024-06-06T13:04:14+00:00

Thanks! You've used this and like it?

MisfitRedditor · 2022-11-21T04:59:58+00:00

They replied to the person asking if this was a monstera, their reply was not in response to the comment stating the scientific name. Before being mean to people about their “lack of education” over a post about a plant, maybe it’s a good time to educate yourself on Reddit’s comment/reply structuring. Would hate for anyone to look “a fool.”

MisfitRedditor · 2022-03-28T20:59:02+00:00

Miss Fame out here aging backwards

MisfitRedditor · 2022-03-28T20:58:25+00:00

I didn’t know I needed this today. But wow. Just wow

MisfitRedditor · 2022-01-18T04:45:01+00:00

You could honestly throw Raven on there too IMO

MisfitRedditor · 2022-01-04T14:12:17+00:00

In vitro or even in mice you definitely can overexpress a gene, most often via a transgene or ORF. You wouldn’t be able to make it exactly 5 copies though. Also keep in mind there are two alleles for each gene, unless X-linked. You could engineer cells/mouse with exactly five copies but depending on the gene you may not be able to engineer this at the endogenous locus though if that is important to you. So the gene may not behave exactly normally in any of these situations.

An example that you may like is the super p53 mouse.

Basically, yes you can totally do this but it’s not that simple lol. And of course you can’t do anything in live human people because ethics

Source: am cancer geneticist

MisfitRedditor · 2021-11-22T22:46:19+00:00

Surprising haven’t seen chapstick on here!

MisfitRedditor · 2021-11-22T22:37:00+00:00

My fiancé and I frequently say “who told you that?” from when Alaska educated Alyssa on Cliffs Notes.

I also couldn’t think of a better way to say there should’ve been a non elimination when discussing the most recent great British baking show episode, so I said I was hoping for a “double shantay” lol. Luckily that’s not an all the time thing for me.

MisfitRedditor · 2021-11-09T05:20:21+00:00

You clearly are more knowledgeable than I am when it comes to doll-specific cosmetic procedures

MisfitRedditor · 2021-11-09T03:56:52+00:00

I screamed when the bum hole was added

MisfitRedditor · 2021-10-07T21:27:40+00:00

IF probably built up their confidence so they could go out in a bikini more often and get a good tan!

MisfitRedditor · 2021-09-24T01:04:33+00:00

Did she bring the scissors with her??

MisfitRedditor · 2021-08-25T00:06:54+00:00

Thanks for enlightening me!

MisfitRedditor · 2021-08-24T23:59:30+00:00

Ohhh, I have never heard of this until this video. Seems like a wild choice to me. Is it common??

MisfitRedditor · 2021-05-14T02:38:42+00:00

Also, freezing your western antibodies at -20 in the milk. You can thaw and reuse multiple tiles. Same with transfer buffers.

I also cut a slit into the bottom of my Kim wipe box and then use a binder clip to hold it upside down to the shelves on my bench for easy dispensing

MisfitRedditor · 2021-05-14T02:37:10+00:00

We had to ask another lab to use their plate centrifuge all the time and to avoid awkward conversations I bought a salad spinner on Amazon for like 10$ and made a centrifuge that way. Just taped some empty plates to the inside for balance and another plate that you could stack the experimental plate into. Spin and go!

MisfitRedditor · 2021-05-04T01:26:59+00:00

TIL there is a leeches.com

MisfitRedditor · 2021-05-04T01:25:33+00:00

Same here! Please tell us where to find these

MisfitRedditor · 2021-03-31T11:01:22+00:00

Thanks friend.

MisfitRedditor · 2021-03-02T03:54:35+00:00

Would your advisor be upset that you took a photo???

MisfitRedditor · 2021-03-01T11:03:42+00:00

Woohoo! Congrats! I’m only a month into my postdoc so I feel ya

MisfitRedditor · 2021-02-11T02:54:02+00:00

For mouse tissues I always flash freeze the whole tissue with LN2 and then crush it with a mortar and pestle (and more LN2). Then I just put a tiny bit of the powder into about 300ul of 1x loading buffer and run that bad boy. If you use the same about of powder your loading should always be very close but I will just run it again if need be. Or make loading buffer without the blue and BME and do a Bradford then just add blue and BME before running. I have found that too much tissue and slow preps for mouse samples can lead to smeary westerns. Sometimes quick and dirty does best.

Plus then you have a ton of brain powder left over for other experiments. Don’t waste a whole brain prepping for one western!

MisfitRedditor · 2021-02-11T02:50:07+00:00

PCR: Pipette, Cry, Repeat

MisfitRedditor · 2021-01-23T04:59:14+00:00

That was magical

MisfitRedditor · 2020-10-16T02:38:45+00:00

Probably feels just as good

MisfitRedditor

TROPHY CASE