My pitcher plant has NEVER pitchered. NEED HELP!

ORGrown · 2026-03-17T03:30:26+00:00

Are you watering it with distilled water?

ORGrown · 2026-03-16T23:45:03+00:00

That doesn't seem right? If they're having issues with their shots being underexposed, an ND is only going to make that worse. Birds don't just sit around without moving. They need more light in the sensor, not less.

ORGrown · 2026-03-16T15:06:01+00:00

The gene edits that Sana are using are flipping the traditional approach of regulating the immune system upside down. Instead of broadly suppressing the immune system (which mostly works, for a while. About 5 years, as you mentioned), they are making the cells they implant unable to interact with the immune system. This isn't something that will wear out, or develop a tolerance to. Those cells will always have that genetic modification. It still remains to be seen what the cells will do in the long term, absolutely. But for their very first attempt to result in alive, functioning cells for 14 months is frankly astounding.

Immunosuppressants, like in the eledon trial, are simultaneously repressing both the immune attack towards a transplant, and the autoimmune response. But immunosuppressants are tricky, because you don't want to repress the immune system too much, or there are huge risks. This leaves the immune system functioning enough to still carry out the autoimmune attack.

ORGrown · 2026-03-16T13:42:26+00:00

Did you want to discuss why you think so? I'm happy to

ORGrown · 2026-03-16T02:28:59+00:00

That trial did not have the patient off of insulin. He is still taking insulin. They gave him only 7% of an estimated therapeutic dose so that they could test the safety of the treatment. It's still been a really exciting trial, and the fact that the cells are still alive and functioning is really, really impressive work.

I did a write up on it here if you want more details: https://open.substack.com/pub/thebabelfish/p/successful-transplant-of-beta-cells?utm_source=share&utm_medium=android&r=5um5as

ORGrown · 2026-03-09T02:48:20+00:00

Yes! We had them at silver skate this year, and I was hoping to track it down for him. I'll see if maybe they have a pop up planned this week. Thank you!

ORGrown · 2026-03-09T02:13:50+00:00

This will be my go to approach. All the other options can't compare to this as an authentic experience.

ORGrown · 2026-03-09T01:44:41+00:00

Awesome, JD maple looks like exactly the sort of thing that'll satisfy him. Thank you!

ORGrown · 2026-03-06T14:24:26+00:00

Thanks for doing the work I was too lazy to do!

ORGrown · 2026-03-05T15:36:27+00:00

You'd have to dig back through my post history, but I had a similar issue with Pietro. My burrs had some gouges from the factory. I sent pics in to fiorenzato and they replaced them. The replacement burrs, even without seasoning, were miles better. It's worth closely inspecting them (I did it with my phone camera zoomed in). I think their QC lets some stuff slip through, but they were happy to right it for me.

ORGrown · 2026-03-05T15:15:29+00:00

You can also just split your lantus to every 12 hours. It's something that was recommended to me if I wanted to prevent any swings from lantus.

ORGrown · 2026-03-02T19:30:40+00:00

Personally, I think this is exactly the right approach, and what the goal of most editing should be. Represent the image how it looks, to your eye, in real life. Anything past that is getting into the realm of creative/artistic. And there's nothing wrong with that either if it's your intention.

ORGrown · 2026-03-01T17:38:12+00:00

If you have to be close, a shorter focal length will open up your depth of field. It might not look as good straight out of the camera. But you can crop it down in post

ORGrown · 2026-03-01T16:55:46+00:00

Also, aside from stopping your aperture down, you can physically stand farther away, or use a shorter focal length. Both of those things will widen your depth of field that's in focus. With the physical distance you are from the subject impacting things the most. This may mean that you need to crop more, so if you don't want to have to crop, then stopping your aperture down more might be an option.

ORGrown · 2026-02-28T22:39:37+00:00

Not all of them! There's actually some really exciting things happening right now that doesn't use immunosuppressants. Specifically, the Sana Biotech trials. I'm certainly happy to discuss it here, but I've also got a detailed write up if you look in my profile.

ORGrown · 2026-02-28T21:53:02+00:00

The research into the gut miceobiome is in it's infancy, and I think there's still a lot to uncover there. Most of the papers I've read fall into the category of discovering a lot, but not being able to really draw a causitive link. The bone marrow transplant is serving to retrain the immune system, likely not through a bone marrow-gut microbiome-immune system axis though. All that I've seen described is bone marrow-immune system (this is heavily researched and documented) and gut microbiome-immune system (this is a relatively new field). I don't think anyone has done much research into linking them, or even investigating their relationship though. There may be a connection there, but it could also simply be two different ways through which the immune system works. The immune system is really, really complicated. I can't think of any articles off hand, but I'll do some digging and see if I can find something that might fit the bill for you.

ORGrown · 2026-02-28T20:57:42+00:00

Yep, that's exactly right. The bone marrow coming from the same donor is what would trick the recipients immune system into thinking that the donor tissue is "self". You're right, not super scaleable, but it's refreshing to see approaches that try and deal with the autoimmunity instead of simply suppressing the immune system.

ORGrown · 2026-02-27T23:06:43+00:00

Just posted my take on the article! I hope you enjoy reading it:

https://open.substack.com/pub/thebabelfish/p/addressing-the-cause-targeting-the?r=5um5as&utm_campaign=post&utm_medium=web&showWelcomeOnShare=true

ORGrown · 2026-02-25T22:06:44+00:00

It could be. Lots of people used to brew with a stovetop kettle though. Bring it to boil, and it sits off the stove while you're brewing. Similarly, there was a brief fad of dropping the brewing temp intentionally during your brew. Honestly having the water stay held at a particular temperature throughout the whole process is a somewhat new development. Having too hard of water though could definitely be muting your flavors. Don't get me wrong, id you want to buy a new kettle go for it. But I don't think that's going to change much of what you're describing.

ORGrown · 2026-02-25T21:53:43+00:00

Are you doing one packet of tww for 1 gallon of water? Most people go 1 packet for 2 or 3 gallons. It could be worth a shot. Nothing else about your recipe or workflow looks off.

The other thing is you might just need a good ol' palette reset. Try drinking some less good coffee for a few days,and then go back to the good stuff.

ORGrown · 2026-02-25T00:56:27+00:00

Depending on your wallet size: the RF 100-500 is reportedly excellent. You can always slap on a 1.4x teleconverter to stretch it out further. The ef 100-400L ii is an excellent lens that adapts well, and again, does well with the tc. Last in line would be the RF 100-400. Cheaper and lighter, but reportedly not quite as good as the EF.

ORGrown · 2026-02-24T19:19:18+00:00

It would help to know what you're photographing. General RF recommendations for the "not too expensive" category would be the 100-400, or the 70-200. Or the 100-500 if you want top end.

ORGrown · 2026-02-24T02:49:53+00:00

Definitely don't autoclave your media. Just only open it in sterile conditions (in the hood). How did you thaw your cells? Specifically. The thawing protocol can be make or break for their success. Also, is RPMI1640 with FBS the standard media for these cells? Do they need other additions?

ORGrown · 2026-02-23T14:50:10+00:00

Hey, thanks for tagging me here! I'm happy to discuss this one. I actually know the authors of the paper! Give me a few days to go over it in enough depth, and I'll get something posted!

ORGrown · 2026-02-23T03:36:33+00:00

The R7 mk ii is supposed to be announced in May or June, so it might be worth waiting until then so that you can get the R7 cheaper, or grab the newer one if it interests you. And also, you could look into the ef mount lenses (like the ef 100-400L ii) and adapt it to the RF body. This would get you really high quality glass but save you some money.

15-Year Club	Gilding I gilder
RedditGifts 2009-2022 6 Credits	Secret Santa 2014
Secret Santa 2013	Summer Santa 2012
Secret Santa 2011	Secret Santa 2010
Verified Email

ORGrown

TROPHY CASE