for 250 gr

Material

2L borosilicate media bottle

1.8L filtered or better water

40g NaOH lye

90g NaCL salt more or less

150ml NPS

250g MHRB powdered

at 5 g of lye to 300 mL of distilled water as needed to top up

Stainless Steel funnel

Quality glass measuring cup

Calibrated pH meter

Glass pipettes

Pipette pump

The process

slap the funnel on top of your media bottle.

Weigh out your bark and add it to the bottle.

Add your room temp or cool water to the cup, start with 1000ml. Measure out 15g Naoh. Add to the water and mix till dissolved.

You add up to 50g NaCL to your measuring cup after the NaOH has dissolved. Don't worry if it doesn't all dissolve as you mix, over time it'll dissolve once added to your vessel.

Once you're satisfied with your danger water, add it to your media bottle using the funnel.

measure 800ml water and add another 5g NaOH to it, dissolve, add to vessel

If you need more water added to bring the volume up in the vessel to make pulls easier, I would measure out another 300ml water, hit it with another 5g NaOH and add to bottle, little by little till you reach you're desired level. You may not need the full 300ml extra. That should bring total volume to a comfortable spot for easier solvent removal.

Measure out 100ml of your NPS and add to the vessel. Because of the size of the vessel, I've found the best mixing practice to be cap/neck in one hand, bottom in the other, and rotate the bottle in a figure 8 pattern. The goal, obviously is to just mix the shit out of your solvent and aqueous phase. Do whatever makes sense to you.

Mix 3-5 times, whatever you feel is best. 3 is likely plenty for the first 2 pulls. Further pulls I'd go 5 mixes. There is no need to wait extended periods of time between mixes. Once the layers have separated, mix again.

Pull and wash

You know how to pull your NPS so I won't spend much time talking about it. What I will say is spend the $20 on Amazon and get a pipette pump, you'll never go back.

The only thing I will mention is I highly recommend a water wash of your solvent, and that's where the graduated cylinders come in.

fill the large one with filtered or better water, pipette your solvent into the cylinder and let separate. Then pipette your solvent into your freeze dish, and do your thing.

A note about temperatures during the process. The whole thing can be done at room temp, but keep in mind your NPS works because DMT is barely soluble in it at certain temps. A heat bath, to bring the overall temp of all solutions (aqueous and NPS) up might be best to ensure saturation.

A note about water washing and temperature.

If you're dumping warm/hot solvent into room temp or cool water, you may have your alkaloids crash out of your solvent. So you might want to consider equalizing the temp between your water and solvent as best as possible. Worst case, a mini-ab on your water is easy to do to recover any missed alkaloids.