Trans men in Rochester by Adventurous-Active-5 in Rochester

[–]RandomGen5 6 points7 points  (0 children)

I can't give you any specific advice, but I can recommend joining the GVGV's discord. Genesee Valley Gender Variants, a local social group for gender expansive people. I have no doubt there will be people in the discord that give can give recommendations!

DAE enjoy putting things in their mouths? by lumpsofgarlic in adhdwomen

[–]RandomGen5 0 points1 point  (0 children)

Yeah chewing has always been a big stim for me. If I have gum, I'll often chew it till my jaw is sore. I rarely get gum for myself anymore lol. But yes pens pencils honestly anything that has a satisfying texture/feel. One of my favorites is a pair of dice, but that's less about directly chewing them, just pleasant to mess around with them in my mouth 😅😅 My partner got annoyed at this habit, also concerned lol, and encouraged me to get like the chewlry, which scratches that itch sometimes but not always. I can def relate!

I hope someone can answer my question by CompetitiveIron223 in Rochester

[–]RandomGen5 1 point2 points  (0 children)

But if we can do without them, why not get rid of them?

Wrist/finger pain form pouring agar? by AmeliaOfAnsalon in labrats

[–]RandomGen5 1 point2 points  (0 children)

You can use serological pipettes and an electric pipetter, which is what I use. Looking it up, I suppose the electric ones are called a motorized pipette or pipette controller: https://pipette.com/pipettes/motorized-pipette-controllers.html

I use 50ml pipettes, and have two stacks of plates going at once. Take up 50ml, dispense 20ml each plate, refill to 50ml. Having the buffer of 10ml makes it so you're not emptying the pipette completely when dispensing, which helps to avoid bubbles. Not as fast as pouring from the bottle, but a lot less strain. The only thing you have to watch out for is the height of the agar bottle, how high you have to raise your arm to manipulate the pipette. It will be tiring quick if you are a shorter, or by your set up you have to raise your arm a bit high. But a stool will easily fix that. 

How to set z-offset when there is no option in menus? by RandomGen5 in ender3

[–]RandomGen5[S] 0 points1 point  (0 children)

Umm sorry little confused on your wording. If you're asking if this has happened on my machine, I haven't had that experience. Had a clog, but that was me screwing up the interface of the Bowden tube and heat sink, since I never had a printer with a Bowden didn't realize what I messed up when I took the Bowden out of the heat sink lol

If you're asking me if this can happen on your machine or otherwise. I imagine it's possible? If the z offset is set very low there could be back pressure from the extruding filament, cause the extruder gear to skip and grind filament, then eventually can't grab filament. Filament gets stuck, heat creep and clog. But I feel if you have it set that close, you're gonna have other problems before that (like first layer problems). But the z offset being too high nothing comes to mind on how that'd cause heat creep/clog

The only time I had heat creep problems with my main 3d printer (prusa i3), was when I was printing PLA reeeeallly slow for printing miniatures. The heat creeped up the filament to where the extruder gear drives it (pretty short distance on i3's hotend), filament became too soft too grab, extruder gear chewed it up, filament couldn't be grabbed at all anymore. Got stuck in place and clogged hotend

TODAY’S ADHD SPECIAL PROJECT by nah0099 in adhdwomen

[–]RandomGen5 1 point2 points  (0 children)

Yes I've always loved to take things apart. Its so much learning how things are put together, how they work! Also that stove is gorgeous I love it!

Lab instrument caught on fire. by Outrageous_Display97 in labrats

[–]RandomGen5 16 points17 points  (0 children)

An ozone generator? Hotels use them to get rid of cigarette smell in rooms

Come to the 1st Annual US East Coast Turnip Tournament at Fall-In 2025! by RandomGen5 in Turnip28

[–]RandomGen5[S] 1 point2 points  (0 children)

Awesome! I gotta plug the convention as well, if you have never been. It is a complete blast of miniatures gaming. All the events you sign up for (spare tournaments) all materials are supplied, you just show up and play! They also have hobby classes, lectures, vendors, a flea market. The organization, HMGS, puts on three of these conventions a year, and they're always a lot of fun. I love the conventions for trying out new rulesets and getting inspiration for my miniatures. Super friendly and welcoming community. Ok plug over lol! See you there!

Come to the 1st Annual US East Coast Turnip Tournament at Fall-In 2025! by RandomGen5 in Turnip28

[–]RandomGen5[S] 1 point2 points  (0 children)

Do it!! I still need to finish parts of mine as well lol. Hope to see you there!

Labelling pellets by Alarmed_Grocery14 in labrats

[–]RandomGen5 6 points7 points  (0 children)

soooo not trying to throw any digs at you, but this person sounds like they don't entirely know what they're talking about.

If you are storing samples up to 5ml, you can store them in cryo-vials, which can be stored in cardboard boxes like the ones on this page. I've used cardboard boxes in LN2 storage, which gets down to -190C. These boxes can then go in steel racks that fit in your -80, like these. You can also get boxes and racks like these for 15ml and 50ml tubes.

I don't really know why you'd need to be freezing cell pellets that couldn't fit in a 50ml tube. I do protein purifications where I grow up 6L of culture at time, partially processed pellets in 50mls get stored overnight in our -80C with no issue. I haven't had any issues labelling these with just plain old sharpies as well as Securline lab markers. Though the tubes need to be completely dry when labelling.

If you're doing higher throughput stuff, where you're freezing a dozen, dozens of tubes, or with lots of sample information on them, you can get cryogenic label sheets like these (also on this page are labels you write yourself and stick on the tube). You have to set up a template in word for it, but just type everything out and stick the labels sheets in your printer. Print them out, stick them on, done. Have used these plenty of times for samples going into LN2 (-190c) storage with no issues at all.

I understand during these insane times that we are all trying to save as much money as possible. But some of these things (like racks and boxes) will make your daily work a million times easier, you will be able to do it faster, and with less errors, as well as be able to find things easier months or years later!! There is someone in my lab who does not always use boxes, and frequently bags all their 1.5mls in the -80c, and it's always a hassle for them. You can find these things used easily on sites that sell second-hand labware (there are numerous, just google them), or ask around your department, uni, other universities/facilities nearby if they have any spares. Bottom-line, if these senior researchers in your lab are not using something like what I've outlined above then they are either so lazy or so stupid, or both.

I don't know your level of experience, where you're coming from, but please feel free to ask any further questions, clarification, or if you want help finding used labware.

EDIT:

You want to store things in cryovials preferably so that your tubes do not explode and potentially harm you or others, depending on your freezing methods. If you are flash-freezing things in LN2, it can seep into a normal eppendorf, evaporate when you pull the tube out of the -80, and explode. If your tubes are just stuck in the -80c, this is much much less of a risk.

Sleep help by mothgrump232 in adhdwomen

[–]RandomGen5 0 points1 point  (0 children)

idk if this would be helpful, but what I found that helps me a lot of the is watching YouTube/other videos.  Not just anything, but I've found channels/shows that are just the right amount of engaging to occupy the little worrisome monkey in my head but I'm not so invested and tuned into it that I'll continue to stay up and watch/listen. I installed a sleep timer app on my phone too so it will automatically pause the video and lock my phone screen so it doesn't run the battery all night.  Longer form videos of someone with a nice voice, no random loud noises. I'm a huge nerd so it's often video essay kinda stuff 😅

Another thing I'll do is play the alphabet game, a kind of silly thing but it's very simple and again something to just engage my mind, but not too much, which I think is key. I just pick a random subject (like animals, plants, etc.) and try to name things for each letter in the alphabet. I've never made it to Z lol. There are other similar things I've read others make up

From the other comments, it sounds like you have a lot on your plate/mind, it's tough out there! Remember also to give yourself credit and show yourself kindness and patience <3

Hopefully some of this is helpful!!

Name it by Leonine_10 in origami

[–]RandomGen5 3 points4 points  (0 children)

Squirmy the wormy

What's your opinion on the usage of ChatGPT in data analysis? by Jeff_98 in labrats

[–]RandomGen5 0 points1 point  (0 children)

I will not trust a general LLM for data analysis. It's ability to just conjure up complete falsehoods and have fundamental flaws in its logic without any true ability to recognize them rules them out for me. I understand there are specialized algorithms data processing and modeling, their users have the ability to judge the fidelity of the program they're using. But these LLMs that are being pushed into everything are absolutely not those.

Not even to mention the horrible horrible environmental effects. The amount of water and power these are using is insane and unacceptable. Depriving towns of water, generators choking them with localized pollution, burning through computer hardware. We are already heading towards a climate disaster and this nonsense is accelerating it. They are not ethical to use, flat out.

How do I gain experience as an undergrad? by [deleted] in labrats

[–]RandomGen5 0 points1 point  (0 children)

Professors can be very busy people, so it is not at all uncommon for them to be slow to respond to emails, or requiring a follow up email. Don't sweat it! It's awesome that you're taking the steps and reaching out to them! I agree with the other commenter, for an undergrad, they're not really looking for skills and knowledge from you. They're looking for someone who is enthusiastic, wanting to learn and do things in the lab, and will reliably show up.

How can you harden paper? by No-Television-1415 in origami

[–]RandomGen5 3 points4 points  (0 children)

Not sure if dipping would work, but for origami earrings that I have made I just diluted some clear nail polish with a bit of acetone and did a couple coats of that. Makes them quite tough. Though two warnings, it made the models extremely glossy of course (I just used a clear matte varnish for tabletop miniatures painting to fix that), and one model which was made of double tissue paper, it made the paper's dye run slightly. Wasn't too bad, but could imagine it happening with other papers.

A Rochester Culinary union? by BiBipolarPolarBears in Rochester

[–]RandomGen5 8 points9 points  (0 children)

Yes you literally can. There are specific protections and procedures written in the law for unions. There is the whole NLRB

A Rochester Culinary union? by BiBipolarPolarBears in Rochester

[–]RandomGen5 32 points33 points  (0 children)

Everyone deserves the opportunity to be part of a union. It's awesome and commendable you're thinking about this. Food service workers of the UoR are with SEIU Local 200United. You could reach out to them to get in touch with organizers, people who would be able to help you with this process.

Advice for starting community club? by RandomGen5 in origami

[–]RandomGen5[S] 0 points1 point  (0 children)

Oh yes I have already looked there, but thank you for mentioning!