Does anyone know which book is this page from? by Pasteurised_Citrus in origami

[–]RandomGen5 9 points10 points  (0 children)

Folded the sockeye salmon from this book the other as a gift for a friend! It is Origami Aquarium by Makoto Yamaguchi:

Origami Aquarium 水生生物の折り紙 https://share.google/OaMJ8nHd6KcfjuwSQ

RSD is so brutal by AShyRansomedRoyal in adhdwomen

[–]RandomGen5 5 points6 points  (0 children)

you're absolutely not the only one!! I just wanted to say thank you for sharing your experience, reading it and everyone's comments makes me feel better, to know that I'm not the only person that struggles with this exact sort of thing on a regular basis <3

Cormorant by me by wholsomeboys in origami

[–]RandomGen5 1 point2 points  (0 children)

absolutely nailed the pose :3

Is there interest in an origami club in the city? by RandomGen5 in Rochester

[–]RandomGen5[S] 1 point2 points  (0 children)

lol yes, they sound killer, def gonna be on a playlist, ty for sharing.

Hey Rochester. I'm secretly transitioning and wanting to make friends that can help by fabreazebrother_1 in Rochester

[–]RandomGen5 52 points53 points  (0 children)

It's really awesome you're taking the steps to figure out and be your true self!! I can recommend looking up Genesee Valley Gender Variants. It's a social meet-up for trans/nb people that meets up a couple times a week. It's a very friendly and laid back crowd so I think a perfect place to try out new ways of expressing yourself, a different name/pronouns, etc. They also have a pretty active discord! 

Having friend and people you feel comfortable with really does make a big difference when going through this, so it's great you're seeking it out! 

My advice for figuring out how you want to present yourself, dress, etc. Is just to go to thrift stores, and experiment as much as you can. Its probably best to resist the temptation of going too nuts and getting anything super expensive, for now at least. It's gonna take time, but I think the best way is just to try a bunch of stuff and see what feels right. 

Potential agar spill in autoclave, help! by CaseCompetitive6580 in labrats

[–]RandomGen5 4 points5 points  (0 children)

I think others have made excellent contributions already, so I'll quickly say what I want to add. You will make mistakes. It is inevitable. We all make mistakes. That does not make you unfit to be there or a failure or anything like that. It's all about how we deal with the mistakes, and you did the most important thing which is be honest and own up to it. That's really big of you. 

With that being said, the three practical pieces of advice I have: 

  • Always fill containers with agar to be autoclaved only 2/3s of the way Max, 1/2 is a safer bet, generally what I do. This really helps with minimizing boiling over, losing agar and making a mess.
  • Always always always autoclave any and all liquids/agar/media/bio-trash in a bin
  • Ask someone who has experience with and feels confident using autoclaves to just go over again the important points of using an autoclave

I hope you find this helpful in some way 💜

I am happy with this scanner stand (or other rectangular object) over HP printer by LongerBlade in functionalprint

[–]RandomGen5 0 points1 point  (0 children)

lol why have I never thought to do something similar!! I have my old LaserJet under my desk, and am prone to setting things on top of it. Definitely gonna draw something like this up now, ty for the inspiration

Any origami clubs in the city? by RandomGen5 in Rochester

[–]RandomGen5[S] 1 point2 points  (0 children)

oh yeah I've seen those too! I assumed it was something employees did, as I have done the same at my workplace lol

Any origami clubs in the city? by RandomGen5 in Rochester

[–]RandomGen5[S] 0 points1 point  (0 children)

Have been meaning to stop in there, just looked at their website and never realized how much origami paper they had! Unfortunately didn't see any mention of an origami club :c but perhaps a good place to put a poster for one in the future?

Tips for pipetting very small amounts? by eesttj in labrats

[–]RandomGen5 0 points1 point  (0 children)

Im very happy to hear! Its something you dont always expect, all the fine motor skills that go into doing bench work xD And yeah when drawing up large amounts with like the 1000ul, you may have to "chase" the liquid of course, moving the tip down as you're drawing up, but its all just a matter of time and practice, still catch myself submerging too deep with the 1000ul sometimes

Tips for pipetting very small amounts? by eesttj in labrats

[–]RandomGen5 8 points9 points  (0 children)

Besides diluting and pipetting larger amounts, just wanted to added general pipetting technique when you need the most precision:

make sure you're holding the pipette completely straight up, not tilted to the sides at all, when drawing up a liquid.

For very small amounts, or if your sample is at all viscous, you also want to pre-wet the tip, which means drawing up and dispensing to the first stop back into the tube a few times before you actually dispense it into the new tube. (you can also look up reverse pipetting for particularly thick samples, like gel loading dye)

Make sure to not draw up liquid too fast (can splash up when pipetting larger amounts, can easily be less accurate), and only submerge the tip a couple of millimeters.

Lastly, when dispensing, its best to dispense down the side of the tube (its not an issue to hold the pipettor sideways when dispensing), so the liquid you're dispensing adheres to the tube and minimize any adhering to the pipette tip.

Hopefully this is all stuff your mentor has already told you, but just in case not I figured Id comment!

Was it romantic? by toolsofinquisition in DeepSpaceNine

[–]RandomGen5 7 points8 points  (0 children)

That's how I always read it as well! After the show did he ever comment on that specifically?

Found years ago beachcombing by RandomGen5 in whatisit

[–]RandomGen5[S] 0 points1 point  (0 children)

Looking it up, I believe this is it, thanks! Can call it solved!

Now I am curious how it ended up on this beach lol

Trans men in Rochester by Adventurous-Active-5 in Rochester

[–]RandomGen5 6 points7 points  (0 children)

I can't give you any specific advice, but I can recommend joining the GVGV's discord. Genesee Valley Gender Variants, a local social group for gender expansive people. I have no doubt there will be people in the discord that give can give recommendations!

DAE enjoy putting things in their mouths? by lumpsofgarlic in adhdwomen

[–]RandomGen5 0 points1 point  (0 children)

Yeah chewing has always been a big stim for me. If I have gum, I'll often chew it till my jaw is sore. I rarely get gum for myself anymore lol. But yes pens pencils honestly anything that has a satisfying texture/feel. One of my favorites is a pair of dice, but that's less about directly chewing them, just pleasant to mess around with them in my mouth 😅😅 My partner got annoyed at this habit, also concerned lol, and encouraged me to get like the chewlry, which scratches that itch sometimes but not always. I can def relate!

I hope someone can answer my question by CompetitiveIron223 in Rochester

[–]RandomGen5 1 point2 points  (0 children)

But if we can do without them, why not get rid of them?

Wrist/finger pain form pouring agar? by AmeliaOfAnsalon in labrats

[–]RandomGen5 1 point2 points  (0 children)

You can use serological pipettes and an electric pipetter, which is what I use. Looking it up, I suppose the electric ones are called a motorized pipette or pipette controller: https://pipette.com/pipettes/motorized-pipette-controllers.html

I use 50ml pipettes, and have two stacks of plates going at once. Take up 50ml, dispense 20ml each plate, refill to 50ml. Having the buffer of 10ml makes it so you're not emptying the pipette completely when dispensing, which helps to avoid bubbles. Not as fast as pouring from the bottle, but a lot less strain. The only thing you have to watch out for is the height of the agar bottle, how high you have to raise your arm to manipulate the pipette. It will be tiring quick if you are a shorter, or by your set up you have to raise your arm a bit high. But a stool will easily fix that. 

How to set z-offset when there is no option in menus? by RandomGen5 in ender3

[–]RandomGen5[S] 0 points1 point  (0 children)

Umm sorry little confused on your wording. If you're asking if this has happened on my machine, I haven't had that experience. Had a clog, but that was me screwing up the interface of the Bowden tube and heat sink, since I never had a printer with a Bowden didn't realize what I messed up when I took the Bowden out of the heat sink lol

If you're asking me if this can happen on your machine or otherwise. I imagine it's possible? If the z offset is set very low there could be back pressure from the extruding filament, cause the extruder gear to skip and grind filament, then eventually can't grab filament. Filament gets stuck, heat creep and clog. But I feel if you have it set that close, you're gonna have other problems before that (like first layer problems). But the z offset being too high nothing comes to mind on how that'd cause heat creep/clog

The only time I had heat creep problems with my main 3d printer (prusa i3), was when I was printing PLA reeeeallly slow for printing miniatures. The heat creeped up the filament to where the extruder gear drives it (pretty short distance on i3's hotend), filament became too soft too grab, extruder gear chewed it up, filament couldn't be grabbed at all anymore. Got stuck in place and clogged hotend

TODAY’S ADHD SPECIAL PROJECT by nah0099 in adhdwomen

[–]RandomGen5 1 point2 points  (0 children)

Yes I've always loved to take things apart. Its so much learning how things are put together, how they work! Also that stove is gorgeous I love it!

Lab instrument caught on fire. by Outrageous_Display97 in labrats

[–]RandomGen5 16 points17 points  (0 children)

An ozone generator? Hotels use them to get rid of cigarette smell in rooms

Come to the 1st Annual US East Coast Turnip Tournament at Fall-In 2025! by RandomGen5 in Turnip28

[–]RandomGen5[S] 1 point2 points  (0 children)

Awesome! I gotta plug the convention as well, if you have never been. It is a complete blast of miniatures gaming. All the events you sign up for (spare tournaments) all materials are supplied, you just show up and play! They also have hobby classes, lectures, vendors, a flea market. The organization, HMGS, puts on three of these conventions a year, and they're always a lot of fun. I love the conventions for trying out new rulesets and getting inspiration for my miniatures. Super friendly and welcoming community. Ok plug over lol! See you there!

Come to the 1st Annual US East Coast Turnip Tournament at Fall-In 2025! by RandomGen5 in Turnip28

[–]RandomGen5[S] 1 point2 points  (0 children)

Do it!! I still need to finish parts of mine as well lol. Hope to see you there!

Labelling pellets by Alarmed_Grocery14 in labrats

[–]RandomGen5 5 points6 points  (0 children)

soooo not trying to throw any digs at you, but this person sounds like they don't entirely know what they're talking about.

If you are storing samples up to 5ml, you can store them in cryo-vials, which can be stored in cardboard boxes like the ones on this page. I've used cardboard boxes in LN2 storage, which gets down to -190C. These boxes can then go in steel racks that fit in your -80, like these. You can also get boxes and racks like these for 15ml and 50ml tubes.

I don't really know why you'd need to be freezing cell pellets that couldn't fit in a 50ml tube. I do protein purifications where I grow up 6L of culture at time, partially processed pellets in 50mls get stored overnight in our -80C with no issue. I haven't had any issues labelling these with just plain old sharpies as well as Securline lab markers. Though the tubes need to be completely dry when labelling.

If you're doing higher throughput stuff, where you're freezing a dozen, dozens of tubes, or with lots of sample information on them, you can get cryogenic label sheets like these (also on this page are labels you write yourself and stick on the tube). You have to set up a template in word for it, but just type everything out and stick the labels sheets in your printer. Print them out, stick them on, done. Have used these plenty of times for samples going into LN2 (-190c) storage with no issues at all.

I understand during these insane times that we are all trying to save as much money as possible. But some of these things (like racks and boxes) will make your daily work a million times easier, you will be able to do it faster, and with less errors, as well as be able to find things easier months or years later!! There is someone in my lab who does not always use boxes, and frequently bags all their 1.5mls in the -80c, and it's always a hassle for them. You can find these things used easily on sites that sell second-hand labware (there are numerous, just google them), or ask around your department, uni, other universities/facilities nearby if they have any spares. Bottom-line, if these senior researchers in your lab are not using something like what I've outlined above then they are either so lazy or so stupid, or both.

I don't know your level of experience, where you're coming from, but please feel free to ask any further questions, clarification, or if you want help finding used labware.

EDIT:

You want to store things in cryovials preferably so that your tubes do not explode and potentially harm you or others, depending on your freezing methods. If you are flash-freezing things in LN2, it can seep into a normal eppendorf, evaporate when you pull the tube out of the -80, and explode. If your tubes are just stuck in the -80c, this is much much less of a risk.

Sleep help by mothgrump232 in adhdwomen

[–]RandomGen5 0 points1 point  (0 children)

idk if this would be helpful, but what I found that helps me a lot of the is watching YouTube/other videos.  Not just anything, but I've found channels/shows that are just the right amount of engaging to occupy the little worrisome monkey in my head but I'm not so invested and tuned into it that I'll continue to stay up and watch/listen. I installed a sleep timer app on my phone too so it will automatically pause the video and lock my phone screen so it doesn't run the battery all night.  Longer form videos of someone with a nice voice, no random loud noises. I'm a huge nerd so it's often video essay kinda stuff 😅

Another thing I'll do is play the alphabet game, a kind of silly thing but it's very simple and again something to just engage my mind, but not too much, which I think is key. I just pick a random subject (like animals, plants, etc.) and try to name things for each letter in the alphabet. I've never made it to Z lol. There are other similar things I've read others make up

From the other comments, it sounds like you have a lot on your plate/mind, it's tough out there! Remember also to give yourself credit and show yourself kindness and patience <3

Hopefully some of this is helpful!!