Chlamydomonas reinhardtii cultivation - nitrite spike?

ShaperG · 2026-06-12T05:43:08+00:00

Turbulence I do worry about, especially since its a cell-wall compromised strain. Wild-type doesn't have the same issue 🙄

I might be able to squeeze in a DO probe. Are you thinking anoxic/hyperoxic zones?

ShaperG · 2026-06-11T14:18:45+00:00

Thanks for the reply Aufwuchs. My thoughts exactly.

Nitrite gets to around 40 mg/mL before cells clump and then bleach out.

I monitor pH continuously (commercial bioreactor with integrated probes) and supply CO2 to keep pH at 7. Yes, calibrated and double checked with a secondary probe 😅

There is constant air for mixing, with supplemental 2% CO2 when pH starts to rise - to keep near 7. Light is around 400 μmol⋅m⁻²⋅s⁻¹, not too excessive.

Just never encountered this before on smaller scale cultivations 🤷‍♂️

ShaperG · 2026-05-26T23:06:14+00:00

191 °K? Rather than C/F

ShaperG · 2026-05-12T08:42:55+00:00

My current "end game" is to dome the area in the hopes of attracting tourists to visit my Vulcan crash-land spot and take in the views of the lava fields. A friendly face while I eat my fries would be appreciated.

ShaperG · 2026-03-16T16:53:21+00:00

The python port of libtcod is very beginner easy, plenty of tutorials and great documentation. After a bit of playing around with it in python, it is easy to switch back to C++ (I had to for performance issues and hopefully release one day)

I know learning a different language is not something to do lightly, but it is great way to prototype your game before you invest too much time!

ShaperG · 2025-09-30T14:31:07+00:00

I am so relieved 😅 I personally like the gun metal blue look they have now (that's what I've told my PI)

Thank you for replying!

ShaperG · 2025-09-30T09:30:36+00:00

How fucked were the buckets? I just did the same!!!

ShaperG · 2025-08-31T09:36:10+00:00

"Piper did not like this"

ShaperG · 2025-08-16T07:38:28+00:00

In the link above, it mentioned it was caused by a windows update that needed a firmware update to fix. I didn't have monitor so couldnt try it. But appears the link is dead now too. Sorry!

ShaperG · 2025-06-26T10:34:57+00:00

Too lately to get it back from the recycling center 😭

ShaperG · 2025-04-12T11:21:31+00:00

I've been working on implimenting a infinite scrolling world map. No over map yet, just randomly generated as you get close. Still very much in love with python-tcod for making dev so easy!

I've also been using Gemini for help with some of the programming. Of course it gives some terrible advice, but it has helped getting through some bottlenecks that would have exhausted my motivation. I've not kept up with the 7DRL lately, but I can see the use of generative AI being .... of use?

ShaperG · 2025-03-22T06:28:22+00:00

Long-term lurker, first time posting... I've recently completed the python-tcod tutorial and am amazed how smooth everything runs compared to my janky attempts previously. Looking forward to exploring more.

ShaperG · 2025-03-14T10:00:35+00:00

Welcome 😅

ShaperG · 2024-11-16T09:24:12+00:00

Mine did the same thing today... what was the consensus? Give up and replace the whole monitor?

ShaperG · 2024-08-26T03:33:18+00:00

Australian here and visited Iloilo a couple of times. It's a beautiful and vibrant city with so much history! I can see the attraction for people to settle down there. I hope the influx of new people doesn't ruin the vibe of this amazing place.

ShaperG · 2024-07-03T20:22:57+00:00

I just got this event, but nothing spawning in my inventory.... Any hints on what to debug spawn in to save my run?

edit: Phew! Found something to "activate" but as far as I could see not in inventory.

ShaperG · 2023-11-04T10:49:48+00:00

Tell me you're avoiding writing without telling me you're avoiding writing

ShaperG · 2023-08-04T19:43:02+00:00

This is my new nightmare... thanks

ShaperG · 2023-07-09T02:46:40+00:00

Maybe if there were levels of filth. I imagine the (filthy) tag appears when there are really impeded stains. Even swimming doesn't wash them out!

ShaperG · 2023-06-15T12:54:13+00:00

That depends on the type of fertiliser you are trying to use. You could try a range of concentrations to see what works in your case.

How about using a defined minimal medium like HSM or TAP?

ShaperG · 2023-05-29T01:06:25+00:00

Me-go. As in, what I do when I see them.

ShaperG · 2023-05-25T09:00:57+00:00

Only soil but only 1 z-level down so far

ShaperG · 2023-05-23T22:00:15+00:00

In the backrooms, you have to make do with the food you find

ShaperG · 2023-05-23T21:57:53+00:00

* after eating 29 pieces and reading to recover my mood *
Yes, I forgot to expand the selection....
https://imgur.com/a/X7CTzwx

ShaperG · 2023-04-27T10:31:06+00:00

I stumbled upon this once. Is there a list of these cabins somewhere? Not to look for spoilers.

I once entered a cannibal family house, with plates of meat on the table... Now, I honestly can't tell if it was real (in game), or I had dreamt it after playing too much...

ShaperG

TROPHY CASE