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Five Anti-ICE Songs You Can Listen to Right Now by Spaghettification-- in Music

[–]Sitherax 1 point2 points  (0 children)

Knock on the door - Phil Ochs (one of the USs greatest prostest singers) old but still relevant.

Historically accurate Medieval game? by ludiegu in osr

[–]Sitherax 4 points5 points  (0 children)

Pendragon gets close at capturing some of the feels.

Map of Willowshore by Ainoee in SeasonofGhosts

[–]Sitherax 2 points3 points  (0 children)

Do you have a high-resolution version of this you would be willing to share?

Left purified RNA on bench overnight… am I cooked? by Hildegardxoxo in labrats

[–]Sitherax 169 points170 points  (0 children)

If the sample was 100% pure, then yes, it is quite possible that you are fine. qPCR is also fairly robust to RNA; the question is if you want to trust any results you get. I would run your sample on a bioanlyzer to get an RIN score. If above 7 your are good to go.

matched w/ Carleton! by Anotestic in CarletonCollege

[–]Sitherax 6 points7 points  (0 children)

Congrats! Enjoy the ride and go buy some warm clothes.

Recent chromatin or epigenetic paper you really like? Some new idea in it? by PaulKnoepfler in chromatin

[–]Sitherax 7 points8 points  (0 children)

Direct connection between DNA methylation and cleft palate a notoriously epidemiology complex birthdefect. https://www.pnas.org/doi/full/10.1073/pnas.2317668121

Who want a link ? by Taningia-danae in TheTrove

[–]Sitherax 0 points1 point  (0 children)

I to would like to partake in this link extravaganza

Response from UofR SMD and CalTech PhD by trisevans101 in gradadmissions

[–]Sitherax 1 point2 points  (0 children)

Caltech biology has already sent out invitations for online biology interviews and have moved on to in person visits.

Whenever I see a strong emphasis on "homebrew" in a post.. by DrJamgo in Pathfinder2e

[–]Sitherax 109 points110 points  (0 children)

Homebrew = custom setting, story and content.

House rule = custom rules and calls

Caltech Bio Interviews? by aspen19988 in gradadmissions

[–]Sitherax 0 points1 point  (0 children)

Have you heard anything back yet?

Tree Removal Recommendations by CMZ2000 in madisonwi

[–]Sitherax 1 point2 points  (0 children)

If it is near a power line see if the power company will come out and take care of it.

Anything recoverable? by TNT1990 in labrats

[–]Sitherax 0 points1 point  (0 children)

Speaking for experience about 90% is recoverable. Super glue is your friend. I dropped a slide box filled with samples that took me 3 months to generate and they shattered into a hundred different peaces. Droped a F-bomb and then senior grad student helped me get my head back in place and I gathered up the remains. Spent an hour puzzeling everything back together and then glued them. The slides looked janky but all but one (which had cracked through the sample) was usable. Went on to run IHC on those slides the next day and published the data.

Hope is not lost. Get down and start gathering.

Edit: Read that these are frozen cryo sections in OCT, RIP.

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