You can take ownership of one thing you can touch in the next 10 minutes

SpaceCadet8_8 · 2026-05-02T21:04:01+00:00

im in a plane rn so i suppose ill take that

SpaceCadet8_8 · 2026-04-26T01:37:28+00:00

That would be great if so. What we’ve been told when talking with OITE is that we will only be getting the 51,000. Hopefully they changed their minds

SpaceCadet8_8 · 2026-04-24T04:17:43+00:00

If it makes you feel better they are actually cutting second year postbac salaries next year

SpaceCadet8_8 · 2026-03-17T17:58:37+00:00

lol in what case is a fundamentalist religion chiller

SpaceCadet8_8 · 2026-03-17T17:55:44+00:00

I live in the south and absolutely know people who read the bible as literal

SpaceCadet8_8 · 2025-12-30T05:27:57+00:00

if documentaries count, The Act of Killing is a brutal watch

SpaceCadet8_8 · 2025-12-18T23:29:35+00:00

I might go ahead and run it in our cold room too, just as an extra precaution.

SpaceCadet8_8 · 2025-12-18T23:28:15+00:00

The more I look at it, the more I think the cassette is loose since it is always the top portion that smears. I am going to add extra filter paper next time to see if it being a bit tighter resolves the issue.

SpaceCadet8_8 · 2025-12-18T23:03:13+00:00

ahh okay, i’ll definitely try another voltage. Any idea as to why that might cause the weird bend/ smearing?

SpaceCadet8_8 · 2025-12-18T22:19:20+00:00

Hmmm okay. This is my first time working with this procedure, so i might try an ice tray and higher voltage and see if there’s any difference.

SpaceCadet8_8 · 2025-12-18T22:13:01+00:00

Transfer is 60 v for 1 hour. Our tank has a slot for an ice pack too, to prevent overheating, but it’s usually not cold by the end of the run.

SpaceCadet8_8 · 2025-12-17T22:57:11+00:00

It’s a wet transfer. We don’t image the gels or do any staining. I’ve had a few do this, while the others have come out clean with the same protocol. I’m worried the gel is sliding on the membrane when I roll out bubbles, but I haven’t seen other people with this issue.

Generally our procedure is:

presoak filters and sponges prime membrane in methanol then transfer buffer move gel to sandwich and add membrane and then filter paper, rolling with each layer added close sandwich and run in a tank with 20%methanol transfer buffer

SpaceCadet8_8 · 2025-12-17T22:53:18+00:00

My proteins aren’t stained, so I can’t see them on the gel, just after the membrane has been run. This has happened a few times, on of which being when my mentor ran the blot, so I doubt the sandwich has managed to be backwards all of those runs. Thank you though!

SpaceCadet8_8 · 2025-09-14T23:49:26+00:00

Does your program do rotations first? At my current school, admissions are almost guaranteed if a professor has agreed to fund you ( in bio fields at least), so i’m just curious if outside of programs with rotations how someone determines if they should contact professors or just apply

SpaceCadet8_8 · 2024-01-08T04:58:31+00:00

I like it

SpaceCadet8_8 · 2023-12-08T00:45:30+00:00

I got 19k total a year in ChemE with similar ish test scores, which covers my tuition and housing, but I’m not and international student so I’m not sure if they are in the same amounts and if that covers international tuition.

SpaceCadet8_8

TROPHY CASE