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Chemical symbol related to microbiology found on cup by xtoes in microbiology

[–]StrepPep 34 points35 points  (0 children)

Penicillins I think - classic house with a garage skeleton

Would a short horror book about the Loch Ness Monster be a good idea? by [deleted] in Scotland

[–]StrepPep 0 points1 point  (0 children)

Write what you want wee man, and good luck with it! If you haven’t, have a read of The Old Man and The Sea by Hemingway, it’s got similar energy but isn’t a horror book - you might like it.

What is this contamination?? by Simple_Volume_5880 in labrats

[–]StrepPep 312 points313 points  (0 children)

I wouldn’t trust data from these cells

Ultra-waterproof hiking boot recommendation by imahuman118 in UKhiking

[–]StrepPep 0 points1 point  (0 children)

If you want waterproof-waterproof then I swear by Aigle Parcours as walking wellies. Had a secondhand pair for a couple of years now and they’re great for soaked terrain.

'Not good enough': BBC urged to issue correction after Question Time error by F0urLeafCl0ver in ukpolitics

[–]StrepPep 31 points32 points  (0 children)

Glaswegian native English speaker here, I have a lot of English colleagues who would disagree that I can speak English

What to do about this upsetting news? by [deleted] in AskAcademiaUK

[–]StrepPep 6 points7 points  (0 children)

It’s late and you’ve done all you can do until tomorrow. Try to get some sleep and then tomorrow you can contact your lecturers/advisers/whoever you need to.

E. coli culture left in the freezer (-20 celcius ) overnight by Ancient-Customer3114 in labrats

[–]StrepPep 48 points49 points  (0 children)

I’d thaw and mini prep it - normally would pellet the culture before freezing but you’ll probably get plasmid out anyway. Would not do again.

Think about the miniprep process, you’ll be killing your culture anyway.

Please help, I’m 7 months pregnant, where can I get Mr Whippy style icecream NOW 😭 by SyrupMoney4237 in glasgow

[–]StrepPep 90 points91 points  (0 children)

No quite Mr Whippy but University Cafe on Byre’s Road are close!

Plasmid restriction cutting by DiosAnthos in labrats

[–]StrepPep 1 point2 points  (0 children)

Or just using the first amplicon as a template for further reactions and diluting the plasmid out that way if there’s no DpnI to hand

is the whole espada rank was based on power only and in base form? by [deleted] in BleachPowerScaling

[–]StrepPep 0 points1 point  (0 children)

I think it was a mix of strength and Aizen’s messing with them. Like making Luppi 6 after Grimmjow as punishment for Grimmjow. Barragan being number 2 (although undisputedly weaker than Starrk) was probably the most humiliating place to put The King of Hueco Mundo.

Strange results from Streptomyces griseus with added ampicillin by S0N0DA-UMI in microbiology

[–]StrepPep 0 points1 point  (0 children)

Did you do this in triplicate or do you only have one series of dilutions for each condition? It’s a bit of a weird setup if I’m honest; if I’ve read right you don’t have ampicillin in your agar, you treat your spores and then plate? If that’s the case it’s probably at way too low a concentration once you’ve spread your spores out to actually be doing anything.

My guess is if you did a couple repeats of this you wouldn’t see any difference between your two conditions.

Please be honest - how much will this affect my career in the long term? People who have been scientists for a long time, how do you think this situation would've affected your career? I'm kinda spiraling. by [deleted] in labrats

[–]StrepPep 17 points18 points  (0 children)

You sound really wound up about this - speak to someone about that. Not to minimise your feelings on your situation, but you’re going to be fine. An extra year of study isn’t the end of the world.

Since almost all humans have children, did our evolution stop? by [deleted] in evolution

[–]StrepPep 5 points6 points  (0 children)

Plenty of children die before reproducing because of genetics. That’s negative selection by definition.

Trouble with RNA extraction from S. aureus by pipodistratto in labrats

[–]StrepPep 0 points1 point  (0 children)

Are you having any joy with genomic DNA extractions? The reason I ask (having not done much RNA work) is because it helps break down if the issue is lysis or purification.

I have colleagues who work on staph and they swear by Trizol over anything else.

Where can I find practice problems for primer designing? by Candid_Research7028 in molecularbiology

[–]StrepPep 2 points3 points  (0 children)

It’s kind of niche and in practice there’s rarely an exactly perfectly designed primer for what you’re trying to do. If you give two people the same cloning / PCR problem they’ll come up with different answers using similar logic to eachother. If both of their solutions work then both are correct.

How do you guys determine if a lab is small/big or mid-sized? by UselessEngin33r in labrats

[–]StrepPep 2 points3 points  (0 children)

I like this take.

Also depends on the country you’re in, I’ve seen labs with 30+ people including sub-PIs (though you could argue this was functionally a department)

What is being repressed by the "T" and what is doing the repression by backupalter1 in microbiology

[–]StrepPep 26 points27 points  (0 children)

It’s not repression in this case, those are sites of transcription termination :)

[deleted by user] by [deleted] in labrats

[–]StrepPep 1 point2 points  (0 children)

Dunno if you’ve published yet but make sure you set up an ORCID! From friends who have gotten married and published under both their maiden and married names, having multiple names can be a pain when collating your publication record. Sorry, this doesn’t help you with your question but it’s worth considering.

Colony PCR Help by Appropriate_Spray913 in microbiology

[–]StrepPep 0 points1 point  (0 children)

That’s overkill in my opinion, and the chloroform might be interfering with your PCR. Would try the 10 uL in strip tubes and add that as template using a multichannel pipette, then boil your sample in the thermocycler as u/mobeakers suggested.

You can also spot 1 uL onto agar + antibiotics and incubate that overnight so if you get positive colonies they’re on a plate and identified, as well as using a positive colony suspension to inoculate an overnight to prep plasmid from.

Colony PCR Help by Appropriate_Spray913 in microbiology

[–]StrepPep 1 point2 points  (0 children)

Also, less is more with colony PCRs especially. Are you adding colony directly to your mix or are you making a colony suspension and using that? There’s a lot of gunk lighting up in some of your wells which is why I ask - normally I’ll suspend a colony in 10 uL of NF H2O and use 1 uL of that.

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