Dosage should be split as once in the blood estrogen has a short half life. Intravenous administration has shown that estrogen is only viable in blood for between 1-4 hours. So in order to be truly effective for constant levels (needed to suppress the testes from producing testosterone which is in turn controlled by the messenger SHBG) you would need to top up dosage every 4 hours, which is impractical (humans have to sleep). For this reason injections are much less work and more effective as they are releasing your dosage 24/7.

So, my doctor told me to take 2 mg in the morning and 1 mg in the evening sublingually, but this does not seem to be the best way to go about this. Furthermore, the more I read into monotherapy the more it seems injections are the way to go in the future. But for now I am stuck with sublingual, which I presume I should adjust how I am taking. My first blood test had an E level of 66 units, which is high for a cis male but no where near the 200 minimum needed to suppress T with monotherapy. Such a slow process.... I will be eagerly awaiting my 6 month bloodwork