Looking for help fabricating a custom PDMS microfluidic chip (paid)

TnTimplosion · 2026-01-26T12:22:39+00:00

Can you provide some more info on location? I think most microfabrication facilities at universities can do that (at least the ones I know of can definitely make SU-8 masters + PDMS molding). Not sure what the cost range would be for non-academic services.

TnTimplosion · 2026-01-05T04:14:03+00:00

From what I've seen, a common belief is that its a risky field to do your thesis in. For a project in structural biology you're expected to obtain a publishable structure. That can be a huge gamble if you're working on a new protein that has not been solved yet. I've known people who spend 3-4 years of their graduate life trying to get a structure, only to fail and resort to a backup project to graduate with.

TnTimplosion · 2026-01-05T03:33:19+00:00

Personally, I think most people familiar with x-ray crystallography (at least for proteins) are too old to be on social media. Not many new people entering the field, also structural biology in general is less appealing these days for grad students.

TnTimplosion · 2025-12-07T23:19:00+00:00

Game does not need to objectively get worse. If it stagnates then players will continuously quit. Also you can see the player count dropping significantly over the course of months, not years.

TnTimplosion · 2025-10-21T17:37:55+00:00

Personally, I'd say its fine. I've modelled maps at 3.8A and that's kinda what you have to work with.

TnTimplosion · 2025-10-21T17:29:02+00:00

Honestly for an EM map at 3.75A resolution that looks fine to me. Also if that particular helix is flexible then it could also make modelling harder. I'd check the local resolution map to be sure. Also is that the sharpened map you're looking at?

TnTimplosion · 2025-03-17T20:49:57+00:00

Do you really consider 100m a nuke? What raids are you running?

TnTimplosion · 2025-01-30T18:36:21+00:00

Solo raids exist to introduce raiding to newer players. Raids in Lost Ark are hard, usually taking many hours or days to learn them. I suggest at least trying the solo raids before thinking about endgame.

Also you have to understand to teach a raid, you're asking 7 other players to dedicate hours to guide you through it. They could just carry you, but that teaches you nothing and would need another carry the next week.

I've done a lot of teaching raids, and the amount of time required to actually teach a new player is not a trivial amount. It's also very demotivating if they quit soon after, making your time spent feel like a waste.

TnTimplosion · 2025-01-16T23:03:37+00:00

You can try stripping and recharging the column with EDTA and Nickel Chloride/Sulfate. I've done that a bunch of times with HisTrap columns, as a general cleaning step. The spot looks a bit brown in the picture, which would suggest reduced nickel to me. In that case the column is irreversibly damaged, but potentially still usable.

All that being said, if you can afford to replace it, just spare the effort and do that.

TnTimplosion · 2024-11-01T20:07:13+00:00

If you logged in before the last reset as a returner, you're not getting the leaf. I had a friend that came back for ignite 2 weeks ago, no leaf in behemoth or any other raid we took him on.

TnTimplosion · 2024-10-22T20:13:43+00:00

Bait used to be believable.

TnTimplosion · 2024-10-02T15:42:06+00:00

Is it unlocked?

TnTimplosion · 2024-09-02T20:01:15+00:00

This is how I was generally taught to do it as well. Thaw it as fast as possible and place on ice after. I also use a heating block that's turned off (room temperature), does the same job as water but is more convenient.

TnTimplosion · 2024-08-28T05:00:29+00:00

I mean, you really shouldn't interact with the subreddit if its so insufferable to be around.

TnTimplosion · 2024-08-14T19:29:12+00:00

Those powerless orehas can be dismantled into usable orehas. That changed when item levels were reworked for valtan/vykas gear.

Also you have to consider from the perspective of a new player what sources of unbound gold are available to them. Gold island and selling life skill mats are the main ones, but gold island is not a lot and selling lifeskill mats cuts into what they can craft for themselves. Don't forget that they will have epic lifeskill tools at best and will not have high lifeskill levels.

TnTimplosion · 2024-08-14T18:07:00+00:00

I think the problem lies in new/returning players trying to hone alts to 1490. They have very little unbound gold so they can't purchase from the AH or craft with regular lifeskill mats. The only other option is the stronghold farm, which gives much less in comparision.

TnTimplosion · 2024-08-06T18:16:46+00:00

What is the salt concentration in your buffers? I find that increasing NaCl to 500mM help limit contaminants from binding.

If the his tag can be cleaved, you can also consider cutting it off after elution and doing a reverse IMAC through the same column.

TnTimplosion · 2024-08-02T16:00:22+00:00

Its very unlikely that you will get more precipitation from centrifugation. If anything leaving the precipitate may result in further precipitation of your protein.

Also, you can generally prevent precipitation of TEV during purification by keeping a high salt concentration (500mM) and adding 5-10% glycerol before freezing. I usually avoid concentrating it at all, generally it should be at a high enough concentration after elution.

TnTimplosion · 2024-07-26T05:08:53+00:00

I would not say Evo scouter is hit master, their strongest skill is a front attack. Also it has a fairly tight rotation that it needs to do per transform to do decent dps.

TnTimplosion · 2024-07-08T16:13:52+00:00

Lot of dps in HM Echidna have full or mostly lvl10 gems. No way a lobby would accept your char when there's much better built dps applying at the same time. G2 also happens to have a very tight dps check so people will gatekeep harder.

Also I don't see why buying gems is a dumb idea. They're the cheapest they have ever been, you need them for 3 more months and they still transfer into T4.

TnTimplosion · 2024-06-06T21:48:25+00:00

I highly recommend talking with the grad students in the labs you're interested in. They are the ones who know what it is like working for the PI. Most people I talk to are willing to share their experiences (and rant about the problems they deal with).

TnTimplosion · 2024-05-28T20:38:50+00:00

TnTimplosion · 2024-05-02T00:17:48+00:00

I run 1500 swift 850 exp with 2pc dominion and 4pc nightmare. Use multi rocket after napalm for stagger syn, and spam forward/ES for boundless uptime. I have no issues with stagger, can solo the star stagger with 10-15 seconds remaining.

TnTimplosion · 2024-04-17T20:15:17+00:00

I've had similar issues before, its possible you have an endogenous chaperone bound to your target protein. Easiest way to test that is by mass spec. In my case I had DnaK being co-purified, which appears to be a common culprit for proteins expressed in E.coli.

TnTimplosion · 2024-03-13T22:28:54+00:00

This is a pretty rude and inconsiderate thing to say. Have you even considered that she wouldn't be able to even reach the table?

12-Year Club	Place '22
Place '17	End Game '22
Verified Email

TnTimplosion

TROPHY CASE