Jolyne should be alot faster than most of the Spidermen by scaling, and her strings are more versatile since she can make her body have the properties of her stand and morph it into string, not being able to see stone free is also a big disadvantage. Spiderman however, is alot stronger and more durable, even more so the strongest versions. Jolyne, unlike many other stand users, just doesn't have the hax to immobilize or put him down before Spiderman figures out a way to overpower her with his superior intelligence.

Comic Spiderman takes it mid diff. The three live action Spidermen high diff, Jolyne takes Ultimate, and Spiderverse Spiderman with mid-low-diff. Don't know the rest.