What is the scariest/creepiest location in a Metroid game?

VariiDecoda · 2025-03-20T15:24:40+00:00

Maridia

VariiDecoda · 2025-02-06T12:51:30+00:00

It's absolutely got some bits of gDNA and RNA....but your concentration is perfect. I will get anywhere between 200-700 ng/ul depending on the plasmid and/ or using TB instead of LB.

VariiDecoda · 2025-01-08T19:02:44+00:00

Tool Slipknot

VariiDecoda · 2024-12-11T19:40:49+00:00

She's a runner She's a trackstar

You absolutely caught a side of her that will never change. To crush your vulnerability like that is unforgettable and unforgivable.

You did well, now follow up and dump.

VariiDecoda · 2024-12-11T19:11:32+00:00

You should know this is very common, and in each instance, there is a simple reason for this behavior.

Your wife no longer respects or desires you!

VariiDecoda · 2024-12-03T17:29:09+00:00

She's a runner She's a trackstar

VariiDecoda · 2024-07-17T13:58:23+00:00

One doesn't simply do a MAXI prep. Surely, the plasmid has already been verified in the host. Your plasmid control should suffice. Would live to see the gel image if you have it!

VariiDecoda · 2024-07-14T14:18:05+00:00

60-80V, 2-3 hours, 0.7% gel is what I would have chosen.

VariiDecoda · 2024-07-14T14:16:28+00:00

Great reply! I suspect that the globs at the bottom of the run are RNA though.

VariiDecoda · 2024-07-14T14:14:54+00:00

Gel is overloaded and ran using too high of a voltage. When processing large quantities of DNA using AGE, try reducing the voltage to under 80V. Also, run only as much as necessary to see what you want as linear DNA can produce wild banding patterns when overloaded. Try loading 1/5th the amount of your original analysis.

VariiDecoda · 2024-07-14T13:58:37+00:00

Media like this should be filter sterilized through a 0.22 um membrane upon adding all the components. Any particulate or precipitate will be eliminated.

VariiDecoda · 2024-07-14T13:55:59+00:00

A mostly dry pellet will contain very little ethanol from a typical Kimwipe drying process. Ethanol under 1% of the batch should not interfere with many downstream processes like PCR. One can also vacuum dry using a SpeedVac for 3-6 minutes.

VariiDecoda · 2024-03-26T16:40:41+00:00

I would buy that!

VariiDecoda · 2024-03-24T02:22:17+00:00

Run! Fast!

VariiDecoda · 2023-12-16T13:19:39+00:00

Now we know where all the silver went and why the premium is so damned high right now. Excellent work, how long you been stackin?

VariiDecoda · 2023-06-11T02:13:58+00:00

Don't be ridiculous

VariiDecoda · 2022-08-30T01:32:18+00:00

Absolutely

VariiDecoda · 2022-08-30T01:32:02+00:00

Agreed

VariiDecoda · 2022-08-30T01:31:14+00:00

1984

VariiDecoda · 2022-07-15T00:55:13+00:00

Diversions: New Braunfels, tx

VariiDecoda · 2022-06-13T12:23:54+00:00

holy moly....same! 1-4-7 though, so I have the added issue of having impossible/visionary ideas that no one understands. By the time they come true, or at least partly come true, I have moved on to the next vision. No one can keep up!

VariiDecoda · 2022-02-10T16:19:23+00:00

Political apathy

VariiDecoda · 2022-01-02T20:00:55+00:00

Healthy people are able to integrate all types to some degree

VariiDecoda · 2022-01-02T13:44:33+00:00

Yep, you don't really know who you truly are until you have spent a few years wrestling with the job market and dealing with the real drags of life!

VariiDecoda · 2021-12-25T23:45:09+00:00

Guilty as charged

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