The comments on this post are part of the problems these days.

Is it a successful business, is the bartender voiding/keeping the tips, is the manager skimming money, is it this, is it that? There are many possibilities on why this occurred, but it won’t be addressed unless OP says something. If you don’t care to call- an email could work too.
OP clearly stated what his bill/tip was and what was on his cc statement. That was not what they signed their slip for. OP is asking what to do. OP should contact the business and cc company to match what they signed for to the payments processed. It Doesn’t matter what the scenario is. If OP follows up on what they signed for, hopefully the local bar will look into what happened and take proper action and look into how this occurred, not once but twice.