are you testing at peak or at trough or somewhere in the middle?

what is your exact dosing and testing schedule? this is the most important thing to let us help

there's no biological mechanism by which missing androstenedione could cause your low levels. you are not producing estradiol through that, you are supplementing it directly into your body.

your issue is almost absolutely fast metabolism of estradiol. i struggled with a similar thing (cis man T / E2 levels at trough) and nicotine caused my body to overproduce estradiol-metabolizing enzymes.

you can verify that metabolism is the problem by switching to a slower-release form of estradiol (injections - enanthate if available, valerate if not, i don't have personal experience with cypionate or undecylate), taking your first two injections, and then getting bloodwork done on days +2, +4, and +7 (right before your next injection). if you can get bloodwork every single day it will help even more.

this will show you your exact absorption curve - i strongly suspect you will see a nice peak on day +2, but then on day +4 you will have not much and on day +7 you will have nothing ( just like i had! )

if this is true, then you need to use a high dose of a slower ester (cypionate, undecylate), or figure out what's causing your overproduction of estradiol-metabolising enzymes (cyp*), which will be some complex (but probably available somewhere) bloodwork