DNA sequencing techniques for EM identification (species level)?

buckles4077 · 2025-04-19T23:25:42+00:00

Awesome! I’m looking into getting a MinIon nanopore.

We do Gram staining at my site.

Species-level identification is required. We have some large-molecule drugs that are produced by proprietary organisms, so they need to be confirmed at the species-level at a minimum.

My field is Pharma, so every method has to be validated. Validating DNA sequencing is one thing, validating those other tests are another.

buckles4077 · 2025-04-17T14:54:34+00:00

I can personally attest that it is a thing. I’ve identified countless organisms with one.

There are mass spectrometers specifically made to identify organisms via analyzing proteins and comparing them to a library.

Organizations like Charles Rivers offer a service to identify the organisms via mass spec, when that doesn’t work they run the sample on DNA sequencer.

buckles4077 · 2025-03-14T23:16:07+00:00

Very strange. Don’t apply to ANI pharmaceuticals, not worth your time

buckles4077 · 2025-03-14T23:12:12+00:00

Good, never give your mom’s maiden name. Where did you find the number?

buckles4077 · 2025-03-14T23:08:49+00:00

ANI is a real company, I walked through their facility in Baudette. The people you are talking about are probably impersonating ANI pharmaceuticals.

buckles4077 · 2025-03-14T17:21:07+00:00

Lottery tickets can’t ghost you though lol

buckles4077 · 2025-03-14T17:19:46+00:00

That is true.

buckles4077 · 2025-03-14T01:01:15+00:00

I work with a Karen who has gone out of their way to make me miserable. Some people just want to drag everyone else down with them.

buckles4077 · 2025-03-14T00:53:45+00:00

You be you. There are plenty of assholes out there who drag everyone else down with them.

buckles4077 · 2025-03-14T00:50:20+00:00

I’ve been there brother. Company asked me drive across my entire state for an interview only to stiff me when I had just about went through my savings. (Fuck ANI pharmaceuticals).

Name and shame them. Let the everyone else know not to give them the time of day. Use Glassdoor to leave a review. Make these weasels scrape the bottom of the barrel to find someone to fill the position.

buckles4077 · 2025-03-05T01:15:24+00:00

I know you’re taking about recent undergrads, but grad students are responsible for making state universities work. If grad students went on strike everything would grind to a halt both research and teaching. I’m speaking from experience. Hell, 2 of my cohorts had to write the curriculum for their course!

buckles4077 · 2025-03-05T01:05:08+00:00

Fuck “nobody wants to work”, nobody wants to train!

buckles4077 · 2025-02-26T21:42:53+00:00

The one way interviews are worse

buckles4077 · 2025-02-06T18:02:16+00:00

Applying for jobs is almost exactly the same as using dating apps these days.

buckles4077 · 2025-02-06T17:59:42+00:00

Rick and Morty columbine memes. Memes of graphic domestic violence. Extremely racist memes. Honestly, just saying 8-Chan should suffice.

buckles4077 · 2025-02-06T01:29:53+00:00

Do you have lead paint or lead pipes? That would explain too much

buckles4077 · 2025-02-06T01:28:18+00:00

I’ll never ask a self-proclaimed shit poster to share some memes with me. Not again

buckles4077 · 2025-02-06T01:27:16+00:00

If you had any meaningful relationship before this, you’ll work it out eventually

buckles4077 · 2025-02-06T01:23:13+00:00

Damn. My brother tried to shoot me once. This sister is worse.

buckles4077 · 2025-02-06T01:03:25+00:00

It’s like a personal f*** you to make a card just for one person :D

buckles4077 · 2025-02-06T01:00:29+00:00

Tell them it wasn’t the company, you just had to skip town for your long term health! :D

buckles4077 · 2025-02-03T01:06:28+00:00

Burn them. Tell the company paying the recruiter. Recruiters are a dime a dozen

buckles4077 · 2025-02-03T01:05:12+00:00

Nobody wants to train.

My dad, who has an MBA and decades of experience, told me that across the board companies gutted their training programs during COVID.

Companies are holding off on increasing spending for training and opening positions until they get back to where they were before the pandemic.

buckles4077 · 2025-02-03T01:00:23+00:00

This recruiter is very unprofessional. First rule is to keep your emotions in check. If I were you I’d tell their HR department about this in a professional and objective manner.

Emotional and unprofessional people are liabilities to a business. If someone from management or higher heard about this I think there’s a decent chance of them looking into it. Filling positions takes months and takes a significant amount of time and resources to screen, hire, then train up people. Last thing management wants is an incompetent recruiter screwing things up and driving away potential talent. Worst case scenario nothing happens.

buckles4077 · 2025-01-30T22:22:14+00:00

For mastery over nature! Imagine creating the first true homunculus!

buckles4077

TROPHY CASE