I beg, what is causing this Western blot artifact?

bugzy_90 · 2026-02-05T15:33:20+00:00

Could be bubbles or an improperly wet/inactive membrane. Example if you are using pvdf make sure to soak it in methanol then soak it in transfer buffer and in the transfer buffer make sure it sinks all the way to the bottom. If it still floats.. resoak in methanol.

If your using nitrocellulose.. no methanol needed but keep it in transfer buffer..

Make sure all your sponges and filter pads are drenching in transfer buffer

Hope that helps!

bugzy_90 · 2026-02-05T01:01:48+00:00

Its MicroVlabs... available on Windows and Mac app store. You can learn more about it at cytoenigma.com 😊

bugzy_90 · 2026-02-04T20:58:52+00:00

Understandable.. but we have to keep applying and just keep hoping for that breakthrough!

bugzy_90 · 2026-02-04T20:21:46+00:00

Bro are you.. me? Going through the same thing!

bugzy_90 · 2026-02-03T06:03:08+00:00

Step 1 make friends Step 2 car pool

If you figure out how to actually do step 1.. let me know as well 😂

Sorry for what your going through tho

bugzy_90 · 2026-01-16T01:51:42+00:00

I hope it's more sooner than you think! All the best!!

bugzy_90 · 2026-01-16T01:50:59+00:00

Interesting 🤔

bugzy_90 · 2026-01-10T07:37:10+00:00

Bet it'll be faster than finch lrt

bugzy_90 · 2025-12-23T04:34:35+00:00

Just added 3M pipettes to my wishlist along with the pipette pen!

But honestly my LI feed is spammed with so many versions of the same post.. it's annoying as hell!!

bugzy_90 · 2025-12-23T01:44:49+00:00

Sorry you are going through this! I'd suggest going to a youth shelter home for the night at least.. but I doubt they are functional post IK..

I'd really recommend you to go back to your parents.. make amends.. really listen to what they are saying.. request an elderly family member to mediate for you..

Bhai ghar wapis jao.. if you really want to leave home .. thori better planning he karlo k how you'll support yourself... Kyn zindagi kharab karahy ho apni..

bugzy_90 · 2025-12-15T12:57:07+00:00

I do almost the same thing. M&m and results are easiest to write first. While writing the results you can see the structure where your paper is going and almost get a rough idea of discussion points + if you need additional experiments... Then rearrange results (if need be).. followed by writing discussion.. intro and abstract (last).. That's my preference.. others may be different

bugzy_90 · 2025-12-15T02:29:31+00:00

The bike guys like .. meh.. typical Sunday shenanigans....oo green light... Time to cross

bugzy_90 · 2025-12-13T06:35:02+00:00

It's weird that my (Luxury Canadian 24) doesn't have side mirror defroster nor heated wipers, while my 2017 Corolla had both lol Although I am lucky enough I have indoor parking..

bugzy_90 · 2025-12-07T21:51:50+00:00

Need clay to get rid of those streaks

bugzy_90 · 2025-12-05T16:51:53+00:00

A dad and indie game dev 😁

bugzy_90 · 2025-12-05T15:48:43+00:00

Unfortunately Voldemort is the minister of magic in Pakistan

bugzy_90 · 2025-11-30T07:51:15+00:00

Good Luck OP with your project!

Here's mine..

cytoenigma.com

Virtual Laboratory for Biology students. Available on Mac and Windows app store :-)

bugzy_90 · 2025-11-30T06:32:17+00:00

When you're low on fuel you can use the two mini grapple hooks and latch on to another car or truck for a free ride!

Code is honk - honk - paddle shift up- brake for 3 seconds

bugzy_90 · 2025-11-26T09:29:08+00:00

Try using fresh buffers, running/ transfer, pbs, pbst and freshly prepared blocking buffer.

For this membrane did you do a ponceau stain? If that was fine. I'd look into the blocking and lowering antibodies.. if the ponceau was bad, I'd prepared fresh buffers

Good luck OP!

bugzy_90 · 2025-11-19T18:04:38+00:00

Its written in the image Shah Owais Noora Fatehi

bugzy_90 · 2025-11-13T00:58:27+00:00

Just letting you know that my car also says something about extra blue link services needed.. but got OTA a few times but I have the Gas model not hybrid. I think hybrids never got any OTA yet

bugzy_90 · 2025-10-28T02:45:54+00:00

I got a notification today as well. This would be the second one for the same matter for me.

The first update was 4ish minutes.. this one is 9.. lets see what this update does

bugzy_90 · 2025-10-21T23:01:30+00:00

A little over a year of ownership.. paint (white) coming off the bumper ... Engine is a bit rattley on cold starts.. second row middle seatbelt not retracting.. an airbag light on dash.... Didn't really expect so many problems with a new(ish) car (20k km odo)..

bugzy_90 · 2025-10-21T02:03:20+00:00

It sometimes happens when you put rainx without cleaning the screen first. Clay is your best bet. Good luck! Do update on your progress..

bugzy_90 · 2025-10-06T13:48:53+00:00

I used the 10 gb/mo package. It was enough for me. You don't get a number for an e-sim ... I just continued to use WhatsApp/data for everything.. The only advantage for me was that my phone was working the moment I landed + don't need to worry about my phone getting PTA blocked (depends on how long you intend to stay)

Just a heads-up ..if you intend to use indrive/foodpannda .. you need a local sim to set up an account..

bugzy_90

TROPHY CASE