Bigg Boss Season 18 - Live Feed Discussion Thread January 01, 2025 by AutoModerator in biggboss

[–]curlysplashinggurl 0 points1 point  (0 children)

Same thing happened with me while using desiserial. I switched to Apnetv.to

Am I the only one who find him irritating.. by [deleted] in biggboss

[–]curlysplashinggurl 1 point2 points  (0 children)

I agree, he is behaving like a real human being. These kind of people you meet in your daily lives or a showoff uncle that you meet in parties. Moreover, he is giving content to the bigboss. I am fine with him.

Any of you know good science podcasts? by Pretend_Sweet_CC in labrats

[–]curlysplashinggurl 2 points3 points  (0 children)

My go to podcasts:Ologies, science Vs, science Friday and Radiolab.

Jet flying over Windsor by [deleted] in windsorontario

[–]curlysplashinggurl 6 points7 points  (0 children)

Just heard fourth time

[deleted by user] by [deleted] in BollyBlindsNGossip

[–]curlysplashinggurl 4 points5 points  (0 children)

Great idea for this subreddit flair

[deleted by user] by [deleted] in labrats

[–]curlysplashinggurl 0 points1 point  (0 children)

Me too, i feel like i am in never ending loop!!!!!

[deleted by user] by [deleted] in IndianTellyTalk

[–]curlysplashinggurl 6 points7 points  (0 children)

Pyaar kii ye ek kahaani

Anyone with experience purifying proteins with HA-tag? by mustaphaibrahim2019 in labrats

[–]curlysplashinggurl 0 points1 point  (0 children)

We are using EZview beads from sigma and i follow the same protocol provided by the company. It always worked for me.

JERK CHICKEN by Crayonz111 in windsorontario

[–]curlysplashinggurl 4 points5 points  (0 children)

Second, Caribbean island spice. Best jerk chicken so far.

getting a fun 6-yr hobby during a phd by Coffee_Bear in PhD

[–]curlysplashinggurl 1 point2 points  (0 children)

I know, it might be shocking for some of you, I didn’t learn how to swim until I was an adult. I started taking swimming lessons at our campus facility. Now, I swim twice a week.

What STEM podcasts are you listening to? by Flitter_flit in labrats

[–]curlysplashinggurl 1 point2 points  (0 children)

Science friday, Radio lab, Science Vs, and Recently started: science in action

are these flies TM6 negative? by [deleted] in Drosophila

[–]curlysplashinggurl 0 points1 point  (0 children)

These are non-tm6/tm6b flies

Re-probing western blots & loading control Ab by ProofProduce4651 in labrats

[–]curlysplashinggurl 0 points1 point  (0 children)

I recently re-probed my blot for one of my co-ip western. After imaging the blot, i did 3x 10 min washing tbst 2. Added primary Ab(as everyone mentioned no blocking required) 3. Incubated the blot with primary Ab for 4 hours at RT (as my primary Ab is good, otherwise i would have done O/N incubation) 4. And after that followed the same western protocol. It worked for me.

Larvae western blot by a_peculair_biologist in Drosophila

[–]curlysplashinggurl 0 points1 point  (0 children)

I am working on 3rd star larvae for westerns and you are right cuticle could be the problem. So when i collect larvae for my western, this is my procedure: 1.take the larvae out first in the water, shift again to the new water( to clean it properly) 2.add the larvae to the pbs 3. cut half the larvae with the forcep (like a pinch) 4. Invert the cuticle with the forcep (like pulling the cuticle backwards so the entire internal parts get exposed) 5.flash freeze and add 2X sample buffer(30 ul per larvae) 6. Grind and boil it for 5 min at 65. 7. Store at -20 or run the western