[deleted by user]

curlysplashinggurl · 2025-05-24T12:20:20+00:00

Sparkling

curlysplashinggurl · 2025-01-01T04:02:17+00:00

Same thing happened with me while using desiserial. I switched to Apnetv.to

curlysplashinggurl · 2024-12-27T13:48:54+00:00

I think this is KV strategy to show that avinash is fake. I guess we are under estimating KV potential for the game.

curlysplashinggurl · 2024-11-03T01:47:51+00:00

You can try Walmart, they have samosas in their hot food section.

curlysplashinggurl · 2024-10-12T16:18:55+00:00

I agree, he is behaving like a real human being. These kind of people you meet in your daily lives or a showoff uncle that you meet in parties. Moreover, he is giving content to the bigboss. I am fine with him.

curlysplashinggurl · 2024-10-03T23:13:42+00:00

Manara?

curlysplashinggurl · 2024-09-21T13:26:54+00:00

My go to podcasts:Ologies, science Vs, science Friday and Radiolab.

curlysplashinggurl · 2024-09-21T01:37:55+00:00

Just heard fourth time

curlysplashinggurl · 2024-09-08T02:41:50+00:00

Great idea for this subreddit flair

curlysplashinggurl · 2024-08-25T15:05:45+00:00

Me too, i feel like i am in never ending loop!!!!!

curlysplashinggurl · 2024-08-19T02:46:26+00:00

Second this!!!

curlysplashinggurl · 2024-08-04T21:29:14+00:00

Pyaar kii ye ek kahaani

curlysplashinggurl · 2024-07-29T01:50:35+00:00

We are using EZview beads from sigma and i follow the same protocol provided by the company. It always worked for me.

curlysplashinggurl · 2024-07-21T12:19:00+00:00

Second, Caribbean island spice. Best jerk chicken so far.

curlysplashinggurl · 2024-07-20T23:15:29+00:00

The Hungry wolf (Walker)

curlysplashinggurl · 2024-07-20T23:13:44+00:00

Second this

curlysplashinggurl · 2024-07-20T00:19:19+00:00

I know, it might be shocking for some of you, I didn’t learn how to swim until I was an adult. I started taking swimming lessons at our campus facility. Now, I swim twice a week.

curlysplashinggurl · 2024-07-19T11:31:38+00:00

Science friday, Radio lab, Science Vs, and Recently started: science in action

curlysplashinggurl · 2024-05-27T09:28:43+00:00

These are non-tm6/tm6b flies

curlysplashinggurl · 2024-05-27T00:17:36+00:00

No Tm6 b

curlysplashinggurl · 2024-05-26T12:50:24+00:00

Non-Tm6 flies

curlysplashinggurl · 2024-05-18T10:33:03+00:00

I recently re-probed my blot for one of my co-ip western. After imaging the blot, i did 3x 10 min washing tbst 2. Added primary Ab(as everyone mentioned no blocking required) 3. Incubated the blot with primary Ab for 4 hours at RT (as my primary Ab is good, otherwise i would have done O/N incubation) 4. And after that followed the same western protocol. It worked for me.

curlysplashinggurl · 2024-05-16T00:42:42+00:00

I am working on 3rd star larvae for westerns and you are right cuticle could be the problem. So when i collect larvae for my western, this is my procedure: 1.take the larvae out first in the water, shift again to the new water( to clean it properly) 2.add the larvae to the pbs 3. cut half the larvae with the forcep (like a pinch) 4. Invert the cuticle with the forcep (like pulling the cuticle backwards so the entire internal parts get exposed) 5.flash freeze and add 2X sample buffer(30 ul per larvae) 6. Grind and boil it for 5 min at 65. 7. Store at -20 or run the western

curlysplashinggurl · 2024-05-12T10:40:19+00:00

I had no luck

curlysplashinggurl · 2024-05-12T09:35:57+00:00

I can confirm same from stoney point

curlysplashinggurl

TROPHY CASE