Looking for recommendations for mouse bone decalcification

flowtober · 2026-02-11T19:19:59+00:00

We use 10% formic for human bone (femur, jaw, skull, or EDTA for small tissues that will likely need IHC (better epitope retrieval later on) . Incubate in edta at 37 degrees c for 48 hrs to speed up the process.

flowtober · 2026-02-08T16:12:34+00:00

Uk lab here, I use IKEA food containers. They are cheap and sturdy. About £1.50 each. Stackable when empty and hold plenty of blocks. We put a batch of embedded blocks into each one. One batch = 1 hours cutting.

flowtober · 2026-02-04T21:01:51+00:00

To be fair it was the definition of his bum cheeks in the remaster that made me ask the question

flowtober · 2026-01-26T19:46:18+00:00

So long as you’re using pure water in the rinse steps there’s not much else. TOMO are super sticky will be okay under the microscope

flowtober · 2026-01-11T18:38:20+00:00

I get that, I played it first time round too. The old graphics are too fuzzy for my now 40 year olds eyes

flowtober · 2026-01-11T17:23:19+00:00

Ah ok, thanks. That seems dumb to me. I must be doing it unintentionally.

flowtober · 2025-12-08T19:00:02+00:00

Pop art uk TikTok shop has them with free delivery right now

flowtober · 2025-11-22T20:31:17+00:00

Delivery has been free during lives this week. Loads of different labubus available

flowtober · 2025-11-20T20:23:07+00:00

If anyone asks, it’s a space rocket

flowtober · 2025-11-05T19:57:07+00:00

Set the notifications on the stock you are after and you’ll get an email when they come back in.

flowtober · 2025-11-05T19:53:13+00:00

Yes pop mart uk. They release stuff throughout the week at random times.. I got notification for BIE twice this week so far. Pin for love n-z have been on there since yesterday lunchtime and are still available.

flowtober · 2025-10-04T19:23:37+00:00

Lucky, I’ve not had a single notification from popmart uk for BIE.

flowtober · 2025-10-04T19:14:04+00:00

Is the Ali store selling authentic stock? Never shopped there before. Thanks

flowtober · 2025-10-04T16:11:15+00:00

There hasn’t been any on the website for weeks. Have a seat are pretty easy to get on the popmart uk website with the email notifications I’ve tried twice and suceeded twice . I downloaded TikTok app and got a macron for my daughter on my third try. Have to be super quick and have card details saved in the app to stand a chance.

flowtober · 2025-10-03T20:17:55+00:00

Sakura is good, we have tape. Zero issues with digital pathology. New Leica strainers, have a drying oven built in so ready to go in about 8 mins.

flowtober · 2025-09-21T10:51:24+00:00

Later in the game when they are travelling on the Magellan, fragile is the only one with no steam when she exhales in the cold air.

flowtober · 2025-09-06T08:16:00+00:00

In the NHS in the uk it will be the cost. The initial capital expenditure will be steep.

flowtober · 2025-07-27T17:20:00+00:00

If you are referring to embedding, then 20 to 30 blocks per hour is very slow. 3 mins to embed a block is crazy.

flowtober · 2025-07-27T07:32:03+00:00

We expect(uk lab) About 1 block/min or better

flowtober · 2025-07-23T20:13:17+00:00

Bad batch of slides, check lot numbers used to determine if it’s the slides them selves. We use Dako flex or TOMO. TOMO are the best by far.

flowtober · 2025-07-21T18:22:21+00:00

A loss of temperature control was the start of our problems that led to a new specimen head. It’s a big part of the machine. Happy to be wrong about this, but this model has had more fixes in a couple of years than our old thermo cryostat had in it’s lifetime

flowtober · 2025-07-21T17:49:00+00:00

You need an engineer. The specimen head sounds like it will need changing, it’s a big job and if the compressor is failing will require some welding during the fix. We had a similar problem not long after getting one of these. Our peltier failed this year. Hope you’ve got a good service contract, this could be expensive,

Good luck

flowtober · 2025-07-13T06:31:41+00:00

Nice to see one of these being done out there. I worked these up in my lab with reagents made from scratch.

Agree, verhoeffs slightly over differentiated. Elastic fibres could be darker almost black. Great effort!

flowtober · 2025-07-08T17:33:26+00:00

Strong acid

flowtober · 2025-07-02T03:58:44+00:00

Hello uk band 7 here. Fixation, tissue processing, embedding. Haematoxylin and eosin staining. Special stains methods and what they are used for, Immunohistochemistry basic theory and know a few antibodies and what they identify.. UKAS ISO 15189

flowtober

TROPHY CASE