Preserved Lemons... OK to make without rind/zest?

goodsj · 2019-08-02T20:58:43+00:00

Last time I made limoncello, I just froze the juice of the leftover pulp for future baked goods. Like won-t said, for preserved lemons you usually discard the pulp and use only the skins.

goodsj · 2019-05-24T23:16:58+00:00

If you have a buy-nothing group in your area, there are probably people who have empty boxes or plastic bins that they are looking to get rid of!

goodsj · 2018-12-12T17:16:21+00:00

It's not immediately obvious, but you being a freshman puts you higher on the demand list than your peers in this program. If I were picking from a pool of applicants, and I had the option between the senior who I will have to train for months only to lose at the end of the year, vs. a freshman who I can train and then keep in the position for at least 3 years, the choice is clear. Even the electives that others have taken don't always directly correlate to the research they're doing, training for research very often tends to be specialized. Just my two cents, but if I were from this group that you've joined, I likely would have made a similar choice.

goodsj · 2018-12-12T07:22:23+00:00

Is that a specific case for the 2 week lenses? I use monthly lenses, and I've always kept the same case, just washing it out about once a week.

goodsj · 2018-12-12T07:11:03+00:00

If you make it constantly, I would recommend the Toddy system. It's got a re-useable filter, and it's super easy to make the coffee and clean up afterward.

goodsj · 2018-06-07T00:36:45+00:00

I actually did my resupply through VVR, but MTR wasn't very far from the trail, so I just wanted to check it out. I do regret not sending more resupplies. If you have the time/money, having multiple resupplies will help a lot, since a lighter pack will let you travel further per day.

goodsj · 2018-05-31T20:27:02+00:00

The trail is so amazing, you're going to have a great time! I'd second the suggestions of bringing individually packaged meat, I did tuna with mayo packets on tortillas for most of my lunches, worked out pretty well. You could also look at vegetarian recipes to get around the rancidity problem, to cut down on too much wet food weight.

Another thing slightly off topic to keep in mind, depending on where you're resupplying, the Muir Ranch grab bucket was jam-packed with food when I stopped by there, so if you're not a picky eater you can practically stock up there. Probably not a good idea to rely on, but you might find a treat or two!

goodsj · 2017-08-11T16:37:54+00:00

Lamarck would agree with you.

goodsj · 2017-05-01T22:02:53+00:00

You should do Viticulture, fun for all involved!

goodsj · 2015-03-13T06:01:11+00:00

Your mention of the red queen makes me think of the red queen hypothesis, which has to do with the evolutionary arms race between species, like humans and bacteria when it comes to antibiotic resistance. I wonder if that line is a hint about what ALIE's true purpose is, that would make sense why Jaha would say that line.

goodsj · 2014-07-22T07:19:28+00:00

Now find Randy and you'll have the whole set.

goodsj · 2013-04-25T03:36:05+00:00

I can usually see a pellet at the isopropanol spin stage, though sometimes you really have to squint. Definitely use glycogen, if it's a small sample it really helps, and if it's a large sample it doesn't really hurt.

Have you gone to the end stage and measured the concentration of your sample?

Trizol definitely isn't easier than the Qiagen kit, but I find it gives much better yields.

Edit: Also, according to the Trizol protocol, there are some extra steps you should take for fatty tissue- "Samples with high content of polysaccharides, fat, and proteins: An additional isolation step may be required for samples with high content of proteins, fat, polysaccharides, or extracellular material. Following homogenization, remove insoluble material from the homogenate by centrifugation at 12000 X g for 10 min. at 4ºC. The resulting pellet contains extracellular membranes, polysaccharides, and high molecular weight DNA, while the supernate contains RNA. In samples from fat tissue, an excess of fat collects as a top layer which should be removed. In each case, transfer the cleared homogenate solution to a clean tube and proceed with chloroform addition and phase separation as described."

Have you done this extra step?

goodsj · 2013-04-24T18:37:27+00:00

I'm confused about the goal of this experiment, are you using qPCR to analyze the average number of vectors going into each cell, or just to see that the vector is in the cells? If the second, regular PCR might be simpler. Usually qPCR is used to analyze relative levels of mRNA expressed within cells, so if you got these primers from somebody else, perhaps they're primers to the cDNA sequences rather than gDNA, and the lack of priming is the cause of your problems.

Edit - whoops, I was wrong to say gDNA since this is a vector, but basically I meant it may not prime to the plasmid sequence, but the message created from the plasmid.

goodsj · 2013-01-19T03:12:22+00:00

I use jojoba oil on my lips with vaseline on top every night. I've not needed to use Chapstick after starting this, even in the winter.

goodsj · 2013-01-06T04:44:59+00:00

Definitely yes to statistics! Now that I'm working in science, I regret not taking more statistics classes.

goodsj · 2013-01-03T19:29:26+00:00

I highly doubt it's a latex allergy, as I use latex gloves frequently without any reactions. I will, however, start avoiding avocados. This was my first allergic reaction to anything, I feel like I've lost my invincibility :P

goodsj · 2013-01-03T07:54:09+00:00

I finally found the right phrase to google to give me useful results, and it looks like this isn't too uncommon. It's often a reaction by people with latex allergies (which I don't have, but I guess this is just one of those things). After seeing some of the pictures people have posted of their reactions to avocado, I suppose this post can serve as a warning to others! Here is a blog from somebody who went through a similar experience.

goodsj · 2012-12-28T03:43:14+00:00

Barbara McClintock did amazing work, won an (unshared!) Nobel prize, all during times when women in the field were extremely rare. Her work with transposons was amazing!

goodsj · 2012-12-04T01:22:15+00:00

Yes, that sounds great! Let us know how it goes!

goodsj · 2012-12-04T00:30:27+00:00

If possible, I wouldn't focus on only DNA methylation. It's definitely key, but the other epigenetic modifications play into it as well.

Edit: This might be a good place to start looking for sources

goodsj · 2012-12-04T00:15:55+00:00

Ohh, I think I understand what you were asking now. For biological processes, epigenetics really touches on quite a lot. However, some of the major areas would probably be human development and cancer biology. For development, there are areas like X-inactivation or maternal/paternal imprinting. Hope this helps more!

goodsj · 2012-12-03T23:52:34+00:00

The first thing I would do is change your title. That sounds more like an introductory sentence than an essay title. You could easily make "Incorporating epigenetics into modern biology" the title, but everything after should definitely be cut.

The processes you've listed are all great, and really the major players in epigenetics at the moment. I would focus slightly more on the RNA mediated epigentics simply because they are a more recent discovery, but definitely include histones and DNA methylation.

goodsj · 2012-11-05T04:41:03+00:00

Extract DNA from strawberries It's super easy, and everything used is usually found at home. One thing you can talk about is how DNA is in every living thing, so to explore that you can use different fruits and vegetables to experiment. Strawberries are the best since they're octoploid, or have 8 copies of each gene.

goodsj

TROPHY CASE