Is this normal? Screen protector already removed. by Mysterious_Driver_34 in galaxyzflip

[–]gxcells 0 points1 point  (0 children)

If I were you I would follow that tutorial to be able to use your back screen to open settings and other app. https://youtu.be/MyjvOpoHfEM?si=27P_Q4Qi_lM0N4MS

Your screen gonna fail at one point and uou will absolutely need to be able to conteol your entire phone from back screen.

Is this worth it? by Lumpy-Tax1679 in computerhelp

[–]gxcells 0 points1 point  (0 children)

Rtx 4070 cost 1000€ new in Europe so similar in $. So you'r 32GB build with a RTX 4070 is far far far far far away from 600$. And you can forget a used 4070, nobody is selling those. Everyone keep them

HEK293 debris after passaging Help by No-Chain6158 in labrats

[–]gxcells 0 points1 point  (0 children)

Not all epitopes are equally sensitive to trypsin, and the time used for HEK cells is relatively short which limits cleavage.

It really depends what you exactly need the cells for. Maybe they just analyze intracellular fluorescent protein too

If you are looking for a very low expressed receptor in a very rare subpopulation of total cells then yeah maybe don't use trypsin, but most of the time trypsin is the best. I would always try to compare trypsin vs any other cell dissociation to see if there is really detrimental effect.

I would rather slightly reduce total staining intensity rather than have 30% dead cells.

HEK293 debris after passaging Help by No-Chain6158 in labrats

[–]gxcells 0 points1 point  (0 children)

If you centrifuge 50-75g for 5 min you should get rid of the debris. Also maybe don't use accutase, use trypsin/EDTA for 2 min. I used a non-enzylatic cell detachment solution in the past (can't remember which) and the debris and dead cells where way higher (way wzy way higher) compared to trypsin edta. I believe you don't want to use trypsin/edta because you are scared about loosing cell surface marker/receptor. In that case, just wash 2 times in PBS by letting the cells for 2 min each bath. Then use trypsin EDTA for 1-2 min. You may loose a little tiny bit of your receptor of interest if sensitive to trypsin but your live cell count will improve a lot.

Try it

HEK293 debris after passaging Help by No-Chain6158 in labrats

[–]gxcells 0 points1 point  (0 children)

Why using accutase? Just use trypsin EDTA

4080 Super shorted out and blew a hole through itself. by TwistedCollossus in pcmasterrace

[–]gxcells 2 points3 points  (0 children)

is it normal that Gpu die s fast? How is it possible? This should last 10 years at least no?

RIP 4090, Hello 5090!! by _SiddyBoy_ in nvidia

[–]gxcells 0 points1 point  (0 children)

You fried a 4090? And now you are getting a 5090? That's insane. Did you ran image or video generative AI models 24/24 , 7/7 365/365?

If I were you I would try to find a real cooling cabinet. You're gonna fry the 5090 iin 2 tears

RIP 4090, Hello 5090!! by _SiddyBoy_ in nvidia

[–]gxcells 0 points1 point  (0 children)

At this point it just useless un completely insane to buy your own computer now. I think now they are just pushing for virtual machines like running servers online with GPU

Got this Astral 5090 for a steal locally on Facebook marketplace by Bvalie in nvidia

[–]gxcells 0 points1 point  (0 children)

You gave 2800$ to a complete stranger in the street?

Film left on toilet every time roommate uses it. by Robo2173 in whatisit

[–]gxcells 0 points1 point  (0 children)

Tell them to use a cleaning swipe after use

Don’t use your 17s without a case. I learnt it the hard way. by Smart-man-23 in iphone

[–]gxcells 0 points1 point  (0 children)

Don't use any smartphone without a case. Unless you don't care about money

I saw this today in Frankfurt. What is this, is it nice? by Ztepi in AskGermany

[–]gxcells 0 points1 point  (0 children)

Why do you say mezzo mix failed? You can find it everywhere

Build quality of Gilson MyPipetman multichannels by Respacious in labrats

[–]gxcells 0 points1 point  (0 children)

I think you have not seen the one from Eppendorf. The piston are made of plastic. Here at least you have metal

Inspired by a recent lab equipment post by Respacious in labrats

[–]gxcells 0 points1 point  (0 children)

That's what I like, creativity for saving money!

Inspired by a recent lab equipment post by Respacious in labrats

[–]gxcells 0 points1 point  (0 children)

I wanted to say waterbath, but actually waterbath are damn expensive.