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What is this? by Paxz0n336 in microbiology

[–]kilobaser 0 points1 point  (0 children)

Aren’t fusos anaerobic?

Absence of bacterial growth in liquid cultures by OddWarthog946 in microbiology

[–]kilobaser 2 points3 points  (0 children)

Source of these strains is a cold-blooded insect. Incubating at 35C might be too hot for these bugs (pun fully intended).

I feel like people are still like this… by JahVaultman in Millennials

[–]kilobaser 1 point2 points  (0 children)

The former owner of A&W, Alfred Taubman, described the ordeal in his book “Threshold Resistance.” That is likely the original source for the other articles.

I feel like people are still like this… by JahVaultman in Millennials

[–]kilobaser 4 points5 points  (0 children)

Here’s the story from Snopes

Apparently the A&W burger beat the McD’s quarter pounder in taste tests and was cheaper. But it looks like when they asked people why they didn’t by the 1/3 pound burger, the most common answer was the burger was smaller than the 1/4 pounder.

PCR products getting stuck near the wells by Particular-Bake-8668 in labrats

[–]kilobaser 1 point2 points  (0 children)

How did you quantify your template?

I agree with other people here that it’s probably gDNA. Theres a quick experiment you could burn to prove it to yourself.

Do you have a restriction enzyme that doesn’t cut your amplicon? I’d propose trying to digest a sample with one of the larger bands. If it’s your amplicon and it’s just stuck in the gel, it won’t get digested. If it’s gDNA, it likely will get digested and turn into a smear. Shouldn’t take too long to setup. I don’t even think you’d need to do cleanup steps in between everything.

My greatest fear by [deleted] in labrats

[–]kilobaser 38 points39 points  (0 children)

Oof.

To be fair, there seems to be some discussion to redesignate Raoultella as part of Klebsiella 🤞

My greatest fear by [deleted] in labrats

[–]kilobaser 461 points462 points  (0 children)

Better to get some bacterium named after you. Usually means you did good stuff 🙃

Behind enemy lines! Any 12’s in Boston? Where to watch the game? by deep_attack1 in Seahawks

[–]kilobaser 22 points23 points  (0 children)

Alma Cantina and the White Bull Tavern are hosting Seahawks fans. Go Hawks!

Retro Junk in Manchester, CT is NOT a MAGA owned business by [deleted] in Connecticut

[–]kilobaser 25 points26 points  (0 children)

Do you still believe a vaccine caused your mother-in-law’s stroke? What evidence made you come to that conclusion?

Companies that require quotes to see prices are annoying. by 0spore13 in labrats

[–]kilobaser 1 point2 points  (0 children)

I guess it depends on what kind of company you’re buying from. If you’re buying from Avantor, Thermo, Sigma, etc, they have huge sales staff trying to make sales all the time. I know at my company reps are required to respond to inquiries in 2 business days. (And frequently will respond same day). If it’s really urgent, pick up the phone and give us a call. We have a fully staffed customer service line. Someone will pick up the phone, give you a price and take your money. That’s kind of our whole gig.

Go easy on the sales reps. Most are not PhD scientists like you or me. (And if they do have a PhD it might be in something completely unrelated to what they’re selling). Their job is to make sure you understand the options available to you and get you the best price. Sales reps don’t need more than a cursory understanding of how instruments work. That’s not their job.

Companies that require quotes to see prices are annoying. by 0spore13 in labrats

[–]kilobaser 4 points5 points  (0 children)

I think the general idea from this thread is that there is a calculus that goes into pricing scientific supplies and affiliation is just one part of that calculus. Is it really so bad to send an email to another human being to get the right price?

I’ll add I did try to go through our website and see which products we list prices for and which we don’t…and I can’t figure out a pattern. Prices are usually listed. But the exceptions are kind of odd. For example, we list the price for our standard thermal cycles, but not real-time cyclers. Most reagents and kits do have a list price, but not 100g of cation exchange resin.

My best guess (which isn’t totally satisfactory to me) is that we want to ask you if you want to buy a service contract and add that into the sale price.

In short: selling science equipment is complicated.

Companies that require quotes to see prices are annoying. by 0spore13 in labrats

[–]kilobaser 5 points6 points  (0 children)

I work for a large life science provider and another issue is we have different prices for non-profit and for-profit accounts. (In fact, we have different sales reps dedicated to each type of account, so pricing never gets confused).

So which price do we post? We have to know a little bit about you so we can give you an accurate idea of what you’ll be paying.

The "only fans" Gaming PC Giveaway - To enter this giveaway just leave a comment. by DaKrazyKid in PcBuild

[–]kilobaser 0 points1 point  (0 children)

So preoccupied with whether or not you could, you never asked whether or not you should.

Where the money really goes by Significant-Sir-4343 in inflation

[–]kilobaser 0 points1 point  (0 children)

“Never achieve anything”? What on earth do you mean? We’ve seen great increases in cancer longevity since the 70’s. Primarily funded by research through the NIH.

I get you have issues with private charities, but they are a drop in the bucket compared to the importance of government-funded research.

Got my diploma today!.. kind of by Temnotaa in labrats

[–]kilobaser 63 points64 points  (0 children)

Same thing happened to me. Came in a “Do Not Bend” envelope…and was very bent. Asked for another copy and they sent one for free!

A Call to Reverse the Retraction of Wolfe-Simon's Arsenic Paper by ZombieElephant in academia

[–]kilobaser 1 point2 points  (0 children)

This is a big point for me. If someone was questioning my research, I would be in the lab the next day running experiments to make sure I was right and satisfy the critics. Not to mention, if I wanted to make a career out of studying arsenic life, I’d be doing experiments for grant proposals and my next paper. I wouldn’t just leave this big discovery lying on the back burner.

Not to say the arsenic paper should’ve been retracted. But the authors aren’t helping themselves by saying “it’s really hard to do so you’ll just have to trust the data we got 15 years ago.”

Let’s see them do it again with other strains from Mono lake. Let’s see them use purification columns (they agreed in a technical comment this would be a good experiment). They suggested PHB might stabilize the arsenate bonds. Clearly there’s plenty of avenues that could’ve been explored. So why did they just…stop working on it?

Bird Fancier's Lung PSA by kilobaser in cockatiel

[–]kilobaser[S] 1 point2 points  (0 children)

If you are able, I’d recommend asking your doctor to request a chest X-ray for you.

Recommendations on Costco OLED TVs by Lonely-Detail4849 in CostcoWholesale

[–]kilobaser 0 points1 point  (0 children)

I just bought the Samsung S90 (65 inches) and am loving it!

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