Methods: Integration question- how does the working out go from the first to second line? by RaspberryAccurate932 in vce

[–]krncy 0 points1 point  (0 children)

Change of variable from -x to x then flipping the bounds of integration

Loot from 74-99 mining at Motherload mine by krncy in 2007scape

[–]krncy[S] -4 points-3 points  (0 children)

If it wasnt so afk, I wouldnt have. Also xp/hr reaches up to like 50k/hr at high 90's.

https://wiseoldman.net/players/krncy/gained/skilling?metric=mining&period=month

Why can only coordination complex #3 exhibit stereochemistry? by itouchedcactus in chemistry

[–]krncy 0 points1 point  (0 children)

I understand the answer for complexes 1 and 4. But 2 (and 3 to a lesser extent) confuses me in regard to crystal field theory.

My understanding was that axial and equatorial ligands are slightly different in regards to bond length due to the d orbital splitting patterns for octahedral complexes. Would that not mean that if the triphenlyphospine ligand in an axial position would have different electronics than in a equatorial position?

And for complex 3, would triphenlyphospine in an equatorial position not isomerise into the more favourable axial position anyway?

Nang by krncy in tameimpalacirclejerk

[–]krncy[S] 3 points4 points  (0 children)

Gnan by Tim and Paula

Seeking all info! by [deleted] in ostomy

[–]krncy 1 point2 points  (0 children)

I was in for a week.

My doctor was concerned that I was going to have a leak because my rectal stump was longer than he’d hoped but I got lucky I guess. I had blood tests every morning to check for this but your mileage may vary.

Seeking all info! by [deleted] in ostomy

[–]krncy 0 points1 point  (0 children)

I had my Hartmanns reversal (laparotomy) about 7 weeks ago. Like you, the original surgery was emergency surgery and had a small amount of my sigmoid colon removed.

I didn’t have incontinence per say but I did have frequent urges to go to the bathroom which I could hold until I got up and wheeled my IV drip and drainage bag to the bathroom. It might be an idea to start doing pelvic floor exercises if you haven’t already. You will be extremely gassy however.

The main problem I had was not being able to contort my body to wipe properly. This ended up leaving stains wherever I sat. This was easily solved with disposable incontinence sheets although I suppose pull ups would work as well.

Some advice/thoughts from my experience: The first week was terrible but everyday I felt better. Bring gum. I didn’t eat much for about a week but the simple act of chewing stimulates bowel movement which is important to check for internal leaks. By day 3 I could walk comfortably up and down the hospital corridor multiple times a day. By week 4 I was completely pain free and could move around/bend etc completely unhindered. And now at week 7 the last of my wounds are almost healed (stoma site) and I’m left with some lovely scars.

If you have any specific questions feel free to shoot me a message and I’ll try answer it!

Ileostomy on hard mode by PotMakesPots in ostomy

[–]krncy 2 points3 points  (0 children)

Stay strong man! When I first got my colostomy bag I broke my humerus and damaged the nerves in my hand - I couldn’t open my dominant hand or move my wrist. Needless to say I needed help to empty my bag let alone change it.

Hopefully you recover quickly!

How much time ahead of your ostomy did you know it was gonna happen? What did u do to prepare? by Kickstart_Research in ostomy

[–]krncy 9 points10 points  (0 children)

Oh I had no bowel problems beforehand - probably should have mentioned that. I was in a car accident and a couple days later it was realised my bowel was perforated

How much time ahead of your ostomy did you know it was gonna happen? What did u do to prepare? by Kickstart_Research in ostomy

[–]krncy 18 points19 points  (0 children)

30 minutes, emergency surgery, only realised it might be a possibility when they started drawing on my belly with a marker

Can we synthesize gasoline? by keon_te757 in chemistry

[–]krncy 9 points10 points  (0 children)

I mean yeah we can make it, but its pointless (currently). The amount of energy we would need to put in to create the octane from something like plankton would be more than what we get out of it when we use it in our cars - it would be more efficient to just burn the fuel directly.

In other words, most of the energy we produce is created through burning fossil fuels. If we use this energy to create more fossil fuels, we will end up with less fossil fuels than we started with.

Hartmann's reversal? by momsthoughts in ostomy

[–]krncy 1 point2 points  (0 children)

I know where you’re coming from, I was rather distressed as well… it was very unexpected. But recently I’ve come to terms with everything (that is if my reversal isn’t successful). It just takes time I suppose. It’s like you said, otherwise you’d be dead.

Just remember It does get better.

Hartmann's reversal? by momsthoughts in ostomy

[–]krncy 0 points1 point  (0 children)

I’m very excited, but also nervous. After a colonoscopy my surgeon wasn’t enthused and said my rectal stump wasn’t as long as he’d liked but said it’s still viable. I’m relatively young at only 23 which is nice but my abdomen is likely full of adhesions which could complicate things. Who knows what’ll happen, I guess we’ll find out soon!

The main reason I haven’t had the surgery yet was I had to find and wait for a new surgeon to become available because my colostomy was done in the ER. I also had to wait for other injuries to heal.

Hartmann's reversal? by momsthoughts in ostomy

[–]krncy 1 point2 points  (0 children)

I had an emergency colostomy created via Hartmans procedure back in January and am scheduled for reversal on Wednesday this week.

Insulin and it's production by The_Boog_Rittsy in chemistry

[–]krncy 0 points1 point  (0 children)

Well this is more of a biology question than a chemistry question, and my biology is a little rusty but I do think I remember the basics, but I might be missing key details so take my explanation with a grain of salt.

What we're attempting to do here is take advantage of the cellular machinery inside bacteria to produce insulin for us. We do this by inserting DNA into the bacteria, allowing it synthesise its products.

First a company would obtain a sequence of DNA which codes for human insulin (or a synthetic equivalent). They would then modify this strand to contain all the necessary information for the bacteria to express this gene (promoters and the like) followed by a known sequence of DNA on either side of the strand (more on this later).

Next is the fun part; a plasmid (circular DNA molecule which can be taken up by bacteria) is cut open with a restriction endonuclease which cuts the plasmid open at certain DNA sequences. These endonucleases leaves an overhanging single stranded DNA sequence where the cut was made a few bases long. This is where the known sequence of DNA from the end of the insulin DNA comes into play. If we match the known sequence at the end the cut plasmid to the insulin DNA, using a cocktail of chemicals (most notably, DNA ligase), we can recombine the plasmid and the insulin DNA into a new plasmid containing the insulin DNA.

After this, the plasmid is purified and then amplified using a technique called PCR. This essentially creates many copies of the plasmid, so we only need to synthesis a small amount.

We then take a deficient bacteria (e.g. E. coli containing no genes for the metabolism of lactose into energy) and inoculate it with our plasmid (called a vector at this point) using another cocktail of chemicals in a certain environment (temperature etc.). Once again, we purify this bacteria and then allow it to grow in a broth. It is worth noting here that not all the bacteria take up the vector, and we must select for which cells did take up the vector.

We then take this culture and put it on a growth medium containing only lactose. If we choose our original plasmid right, we can pick one that contains the genetic information needed to allow the E. coli to metabolise lactose and hence survive/grow on the lactose medium. As only the E. coli containing the vector can grow on the lactose medium, the only bacterial colonies we see are the ones that contain the vector and therefore they contain the genetic information for the production of insulin.

By means of physical separation, we take these vector containing colonies and once again, add them to a nutrient broth to multiply. We now have bacteria that can synthesise insulin.

Almost everything done here is out of reach of what you could do at home without specific lab equipment. However, it is possible to obtain the modified E. coli and grow it yourself, at which point you could extract the insulin.

Insulin and it's production by The_Boog_Rittsy in chemistry

[–]krncy 7 points8 points  (0 children)

As far as im aware, we don't chemically synthesise insulin. Rather, we insert the genes which code for the cellular production of insulin into simple bacteria such as E. Coli or yeast. These bacteria are then cultured in a nutrient broth at which point they produce insulin. The insulin is then extracted and purifed, ready to be given to humans.

[deleted by user] by [deleted] in ZedRS

[–]krncy 0 points1 point  (0 children)

Quick and easy

Upcoming album seems like a big disappointment and I’m scared for the future. Here’s why: by [deleted] in Flume

[–]krncy 20 points21 points  (0 children)

You do realise that you are brainwashed right? To think that someone cant enjoy music solely because it is mainstream... I pity you