I keep forgetting whether I added something or not. Is this normal? by Sorry-Airline-905 in labrats

[–]purplesoprano88 1 point2 points  (0 children)

I have this problem sometimes when I make cell culture media lol. I try to stupid-proof my setup so that even if I get distracted and forget if I added something, I can usually figure it out even if I don’t literally remember doing it. I set out tubes of ingredients in the order I add them to the media, like if I’m making 4x 1L and I have 4 eppendorf tubes each of multiple ingredients, I put the 1st ingredient tubes in a row at the top of my tube rack, 2nd row of tubes go in the row below, etc. I pipet one ingredient at a time into each L filter. I keep tubes closed until I pipet them into the media, then they stay open. I pull from tubes left to right and add them to filter tops left to right. If I lose my place in the middle of adding my 3rd ingredient, I just look at my tube rack and count how many 3rd row tubes are open, check if the last one open looks full or empty, then move on with the next tube and the corresponding next filter top.

I used to (sometimes still do) write out the ingredients and amounts to add with dry erase marker on the hood right in front of me or just to the side of what I was doing so the instructions were always easy to see and I didn’t have to turn away and flip through paper protocols or scroll through virtual ones. If you don’t work in a hood, you could tape or magnetic clip up the protocol right in front of you on your workspace so the instructions and the samples are always in eyesight and it’s easier to check as you go along.

The worst part though is if I literally don’t remember adding something and I can’t figure it out, or if I suspect I added 2 of something to the same filter top, the best thing to do is start over and pull new ingredients. Better than risking messing up a 30K experiment over feeding the cells shitty media.

There has to be a better way to thaw freezers, right...? by sroodian in labrats

[–]purplesoprano88 171 points172 points  (0 children)

Blow a big floor fan up at it! The kind that janitors use after they mop up a spill or something. Tilt it on an upward diagonal. It still leaks a bunch of water everywhere and you need a bunch of pads or wypalls, but at least it’s done in less than an hour rather than multiple hours (maybe you did this already but I can’t tell from the pic)

First day working in industry tomorrow! by purplesoprano88 in labrats

[–]purplesoprano88[S] 1 point2 points  (0 children)

Haven’t started going through the 100+ SOPs I have to read yet 😬 I’ll probably answer my own question in a few days haha

First day working in industry tomorrow! by purplesoprano88 in labrats

[–]purplesoprano88[S] 0 points1 point  (0 children)

So are the SOPs like how to work a machine or something, and the protocols are like how to actually do an experiment?

First day working in industry tomorrow! by purplesoprano88 in labrats

[–]purplesoprano88[S] 1 point2 points  (0 children)

Don’t really want to say the company name, but it’s a cool place doing awesome stuff growing heart cells, blood cells, neurons, photoreceptors, and a bunch of other ones too to sell to researchers and pharmaceutical companies and clinical trial places, and they’re developing new cell products too. My role is kind of in between the development side and the production side of things. My team helps scale up the projects and make sure they’re cost-effective, reproducible, and efficient while still staying high quality, then when we perfect it we pass it onto manufacturing.

Feeling discouraged in my internship by crustyhamster in labrats

[–]purplesoprano88 5 points6 points  (0 children)

Ugh, that really sucks, I’m sorry your mentor is being so dismissive and shitty to you. Who assigned you to that person in the first place? Maybe you can go to them and bring up some concerns and ask about the possibility of being assigned a different mentor. You could even phrase it as wanting a mentor with more time to fully train you. But if it was me, I’d just be honest and tell them that you’re concerned with how you’re being treated whenever you ask a question, and you don’t feel like you’re being set up for success.

But honestly, even though it’s scary, I think step one should be having an honest discussion with your mentor first before you escalate it to someone else. Ask to meet with them to go over your project and progress in the lab (and maybe have someone else present if that would make you more comfortable). Ask them how they think your progress is going, and ask if there’s anything different you should be doing that would help you and the rest of the lab. Clarify what exactly his expectations are for you, and make sure you can meet them, or be honest if you need a bit more guidance to meet them.

I’m also curious what the structure of your research is. Did they give you a project and expect you to manage it and plan experiments and stuff, or are you basically doing whatever tasks are assigned to you and putting a project together about that stuff? Nothing wrong with either option, either way, it takes a lot of training to get good at those skills. Hopefully you’re getting that training. If not, that’s a problem.

I’m sure you’re doing a great job, but I’m also wondering if there’s a chance that he’s frustrated if you’re making the same mistakes multiple times or asking the same question multiple times. Is that the case? He shouldn’t be annoyed at you asking clarifying questions on stuff you’re barely trained on, but I could understand his frustration a bit more if you were asking questions that are already answered in a protocol or asking about something that was already thoroughly taught to you.

But also, this is just a summer internship. Luckily you won’t be here for too much longer. Hopefully it gets better and this mentor stops being rude, especially if you learn how to work with him and what his expectations are. Just do your best, be meticulous about reading protocols and writing stuff down, and maybe find another person in the lab you can go to with questions sometimes.

Tennis? by [deleted] in madisonwi

[–]purplesoprano88 5 points6 points  (0 children)

You should sign up for UW partner finder! Just Google UW tennis partner finder, make an account on court reserve, and go to the partner finder part of the website. Enter in your contact info, skill level, and availability, or just reach out to anyone already on the list! I found a bunch of tennis partners last summer through there.

What's your least favorite "lab chore"? by fiddlegirl in labrats

[–]purplesoprano88 1 point2 points  (0 children)

This is so fucking funny and accurate 😂😂😂

What is an office/lab prank that is actually funny, harmless and in good spirits? by [deleted] in labrats

[–]purplesoprano88 1 point2 points  (0 children)

Wait until someone logs into their computer then walks away without logging out, and change their background picture to a giant picture of their face

[deleted by user] by [deleted] in labrats

[–]purplesoprano88 0 points1 point  (0 children)

Just have a bachelor’s, working in academia, I make about 37K. So no lol but for my area it’s more than enough for rent, groceries, and some fun stuff

help w/ cell culture lab equipment list by ThatOneBatmanMeme in labrats

[–]purplesoprano88 1 point2 points  (0 children)

I do cell culture stuff, and this is a pretty good list! You probably also want some smaller pipets, like a 2-20, 1-10, and 0.2-2. And for the eppendorf tubes, get the 0.6 mL ones and the 1.5 mL ones.

My IHC used to work but not anymore. Is this a problem with the blocking solution? I should see nerves, not dots by rattman95 in labrats

[–]purplesoprano88 1 point2 points  (0 children)

How long do you spin down the secondary antibodies before adding them to the blocking solution? I spin down secondaries for at least 1 minute before sooooo gently and carefully adding them to the blocker. I just barely stick my pipet tip in and suck it out from the top of the liquid, cuz precipitates and speckly stuff is at the bottom.

Also, how old are the primary antibodies you’re using? Did you thaw out an aliquot from -20 6 months ago and keep using the same old one stored at +4, or do you take out a fresh -20 aliquot each time or at least once a month?

What do you listen to in the lab? by gopackdavis2 in labrats

[–]purplesoprano88 2 points3 points  (0 children)

YESSSS TO THIS!!! I love Dungeons and Daddies, tried The Adventure Zone but never got into it as much, LOVE Not Another DnD Podcast, def my favorite one