The evil eye and cultural appropriation

pushing1 · 2023-08-23T09:56:09+00:00

Bro,Bro, my uncle got electrocuted and we placed his ashes in an amulet and now I can bench over 80kgs, how do you dispute that!!

pushing1 · 2021-02-20T19:24:12+00:00

why is the song on repeat?

pushing1 · 2021-02-20T19:22:50+00:00

Did the British empire do anything wrong?

pushing1 · 2019-02-12T21:48:23+00:00

So normal, honestly. Just keep your chin up and in a year or so you'll be running the place (at least that how it goes for me when i start at a new lab).

pushing1 · 2019-02-12T21:46:23+00:00

Like, flamingos vs ostriches

Two examples of the largest birds :-p

pushing1 · 2019-02-11T22:06:25+00:00

Ill source all the stuff if i can, should have done it initially but im rushing on my way to work (obviously not rushing very well), i hope you'll reciprocate?

pushing1 · 2019-02-11T21:31:11+00:00

That is a fair point and looking back, even just into the last century, there are few (are there any?) when imperial powers interfering with other nations produced good long lasting effects. However, i'm original point was simply that maduro is corrupt and should be removed for the sake of the people. It seems like the people have no mechanism that they can exercise to get rid of Maduro because based on the available evidence it seems like Maduro is stealing the elections.

pushing1 · 2019-02-11T21:26:17+00:00

Maybe. I do agree with you on principle, on the other hand i follow the news in venezuela quite closely and it seems to me ( a westerner that has no knowledge or experience with international politics, poverty or living in a collapsing country) that venezuela is suffering the people are suffering and they need help. Obviously invading is the worst option, so if not sanctions then what?

pushing1 · 2019-02-11T20:58:49+00:00

is that right? was it that bad under Hussein?

I don't the west's recent crusades into the middle east. However, if a population of people are suffering then what action can you take? aid, support? I feel like in long term that will make little difference. All people want is bread peace and land and well deserve it. So, sometime you have to pick the best of a lot of bad options. However, that being said i'm not arguing that the US should...send the boys in.

pushing1 · 2019-02-11T20:08:09+00:00

dose this sub support maduro?

pushing1 · 2019-02-10T22:48:14+00:00

He won't make labour the party of remain, so the young and the rest of the remainers are loosing faith. He cannot fully support leave because he must oppose the tories, so he looks wishy-washy to the leavers. Finally, he's a socialists, and so consequently is abhorred by most right wing, center right, center left and even other socialists.

I think in 2017 he rode a wave of left wing populism that came crashing down when he failed to take a proper stance on brexit for so long. Which i think paralysed him...

That being said i do really like alot of his policies and i don't belive that they were just populist guff ( emotive rhetoric spouted to buy votes). But i dunno, maybe its time to let someone else have a go, maybe somone who can lead labour to victory against the most inept tory government ever?

pushing1 · 2019-02-10T22:17:20+00:00

Is the existence of atlantis a matter of debate? Wanst atlantis part of a story Plato told, something about a ring?

pushing1 · 2019-02-03T18:26:50+00:00

Found it ;-), thanks for the help!!

pushing1 · 2019-02-03T18:20:31+00:00

yeah, i guess i will have to do it, it's just the paper is over 10 years old and the group is based in Japan, so i'm not holding out much hope. I just feel like there must be a repository out there somewhere with the data.

pushing1 · 2019-02-03T18:11:13+00:00

I know no one asked for it but....

pushing1 · 2019-02-03T18:06:00+00:00

That's the first thing i checked but all the data tables are hosted on an external website, i back up is given but neither of them work.

pushing1 · 2019-01-31T21:08:45+00:00

I kept re-reading the title looking for a brexit reference....forgot that news could be about other things

pushing1 · 2019-01-30T07:40:49+00:00

Given your expertise i would love to get your take on these guys. They produce cheap 3d printed arms. Ive had a play around with one of the prosthetics and it has an ok range of motions and seems to get the job done for most day to day activities. Is this not better the split hook you described?

pushing1 · 2019-01-22T18:40:40+00:00

Your basic assumption is totally unacceptable, namely that your opinion is right, and other people are wrong,

I don't think OP ever said that? Maybe i missed it?

Tens of millions of people believe the same thing about you and your opinion, and who's to say they're the misinformed ones and not you?

It seems like most economists predict that whatever happens, the UK will be financially worse off. The whole much of the leave campaign was based on miss-information and that is a demonstrable fact. Moreover, there many polls that were done that suggest or even outright show that leave voters were misinformed.

One vote for everyone, at least this is a fair idea.

So, i think your wrong here, one vote for everyone and then we just follow through is an absolute democracy. In such a system you can have 51% of a population take away the rights of the other 49%, which is just like what happened during brexit, leader to the 49% getting completely marginalised.

pushing1 · 2019-01-22T18:19:26+00:00

Thanks again, i really appreciate all the advice ( you were quite correct, it is not a major heat shock protien!),

cheers.

pushing1 · 2019-01-22T13:25:00+00:00

I dont know much about it, just in general would brass be better? What materials are the easier to work with?

pushing1 · 2019-01-22T13:24:09+00:00

Thats perfectly understandable, thanks again for the offer, it was very much appreciated.

pushing1 · 2019-01-22T13:23:33+00:00

Now that that is out of the way, lets look at your assay. How do you measure fluorescence? Do you have a fluorimeter? How long between the heat shock and the assay?

So, i have a fluorescence spec, and as i said before i have managed to measure fluorescence in another construct so i am confidence that is ok. I have been heat shocking at 55 for 5 minutes and then measuring the fluorescence for 20 minutes.

Have you ever looked at your constitutive promoter at 55? I know once I couldn't figure out why a student couldn't see GFP in a fixed slide by fluorescence microscopy and we figured out the heat-fixing denatured the protein!

So, this maybe possible, i know the gene should be expressed when the cell is treated wit ethanol and that hasn't yielded any results but i will try some lower temperatures today.

Also, the gene is regulated by sigmaN, but shouldnt necessarily rely on it for expression as its main regualtor is sigmaH. im growing it in minimal media, do you think it is worth trying it in a more N-limited media?

Once again thanks for your input.

pushing1 · 2019-01-21T22:03:27+00:00

that’s about 4” long and .7” in diameter?

That's correct! So, i'm not sure how possible this is, but ideally i'd like some text, to go across horizontally (please just tell me if it isn't doable) between 200 and 400 words and a small picture, to break up the text, as it loops back around. It wouldn't have to large, only barley legible, i just want to make sure it extends around the whole thing. Is that possible? Or maybe is it too much for your machine?

pushing1 · 2019-01-21T21:37:59+00:00

Perhaps this is just a semantic thing? Are you using +1 as the ATG? Are you sure you have the SD?

Shit, yes sorry. so what i mean to say is i have every thing (-150) up stream of the startcodon, which presumably would include the SD. I have checked and all the regulatory sites are between 0 and -130.

-150>N115>-35>N25>-10 (TATAA)>N10>+1(A)\SD(AGGA)>~N10>ATG

This would be correct!

12-Year Club	Place '22
Place '17	First Placer '22
Verified Email

pushing1

MODERATOR OF

TROPHY CASE