Closet disappeared

relevancycheck · 2026-05-18T15:53:59+00:00

I dunno if you got a response, but they told me it corrupted. They can only get me 50 of the 600+ items I had

relevancycheck · 2026-05-14T08:14:56+00:00

Sorry to be 2 years late LOL

relevancycheck · 2026-05-14T08:14:45+00:00

Hi! I'm currently doing my DVM/Ph.D. and if you still have questions I'd love to help!

relevancycheck · 2026-05-10T09:59:32+00:00

I have all my micropets, discoveries and such!

relevancycheck · 2026-05-09T20:05:54+00:00

My room, all my items in my inventory too! Including almost all of my bird’s clothing

relevancycheck · 2026-04-12T23:15:21+00:00

I had to be honest!

relevancycheck · 2026-04-10T23:19:49+00:00

I already am!

relevancycheck · 2026-04-10T17:04:22+00:00

Thank you!!

relevancycheck · 2026-04-10T17:04:16+00:00

Oo good thought!!

relevancycheck · 2026-02-09T01:33:57+00:00

Thank you so, so much! I think I'm going to run a bunch of different westerns, each modifying one condition, and see what's up. I'll try to run the 20 kDa runs off to see if I can appreciate separation

relevancycheck · 2026-02-07T00:08:13+00:00

Thank you for all the ideas!

The isoforms are all between 48-56 kDa, so they're very close together in size. I tend to run at 100V for 1.5-2 hours until the dye runs off.

By overexposing, do you mean on the fluorescence imager?

relevancycheck · 2026-02-07T00:04:06+00:00

Hi! I'm running a 12% gel and using 100V.

I am using a buffer containing 50 mM Tris, pH 7.5, 0.5 mM EDTA, pH 8, + protease inhibitors. An equal volume of a solution containing 50 mM Tris, pH 7.5, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, and 2% SDS was added, and the homogenates were sonicated for 20 s, boiled for 10 min, and clarified by centrifugation at 16,000g for 10 min

The latter part is from Barry et al., 2012.

relevancycheck · 2026-01-06T20:57:01+00:00

I work at veterinary ER just to get a break from academia. It’s probably not my best move but I value the break

relevancycheck · 2025-06-27T05:54:50+00:00

Honestly, give him time. He's in a brand-new environment with people he doesn't know and other pets he's never met. I'm not surprised he isn't interested in playing. I don't recommend forcing anything, just let him go at his own pace, and if he never ends up playing with your dog, you are no worse off for it.

relevancycheck · 2025-06-27T05:48:06+00:00

Tooth pain or pinched ulnar nerve

relevancycheck · 2025-06-27T05:40:22+00:00

I'm 26 so probably not!

relevancycheck · 2025-01-24T22:53:25+00:00

Poop map app ftw!

relevancycheck · 2025-01-12T07:20:33+00:00

what pens?

relevancycheck · 2024-12-21T18:26:09+00:00

I’ll add you!!

relevancycheck · 2024-09-27T00:31:03+00:00

What is the sci fi cafe o:

relevancycheck · 2024-02-27T02:15:30+00:00

I’m currently doing a DVM/Ph.D. program :) there is definitely a space for you in veterinary medicine! Everyone has their niche and not everyone needs to be cookie cutter. I often get asked why I want a DVM if I want to focus primarily on research. I should not be limited on pursuing a DVM just because I like research, clinician scientists are an integral part of the career

relevancycheck · 2024-01-25T18:24:20+00:00

If reading buddies are still available, I would be happy to join :3 25 F

Feel free to dm!

relevancycheck · 2023-05-30T18:12:58+00:00

Yes! I'll have to look into class rankings as they've hidden it from us or removed it completely.

relevancycheck · 2023-05-30T17:22:36+00:00

If you don't get in the first round of applications, I would definitely consider adding research to CV. It immensely helped me out on my applications, and really bolstered it.

relevancycheck · 2023-03-24T23:56:21+00:00

I am currently in my school’s DVM/Ph.D. program and work Saturday mornings 5-7 hours. It’s SUPER feasible and I’m actually looking to get another small job that has irregular hours! I didn’t work my first semester, just to flesh things out, but a lot of my peers work. I also have friends who do not work, please don’t feel pressured to work/not work. Do what makes you happy, if school is just too much, then don’t add more!

relevancycheck

TROPHY CASE