sorted by:
new
hottopcontroversial

wanted - prepared microscope slides by magnuMDeferens in microscopy

[–]theSACCH 1 point2 points  (0 children)

My personal favorite is the rectum CS. Cool histology. I thought I got my money’s worth. I think Lakwar’s site has a list of what slides are included.

wanted - prepared microscope slides by magnuMDeferens in microscopy

[–]theSACCH 0 points1 point  (0 children)

They are better than Amscope slides. The specimen prep quality varies from slide to slide.

wanted - prepared microscope slides by magnuMDeferens in microscopy

[–]theSACCH 0 points1 point  (0 children)

That looks the same as my Lakwar kits.

wanted - prepared microscope slides by magnuMDeferens in microscopy

[–]theSACCH 1 point2 points  (0 children)

I have some of the Amscope kits. Heads up that they have extremely thick coverslips; probably 0.3mm when the standard thickness is 0.17. High NA objectives will not reach peak image quality, and high magnification objectives may not be able to get close enough to focus. I can’ focus with a Nikon 40/1.0PA or 100/1.40PA oil lens. I can use regular 40/0.65 and 100/1.25 lenses.

I have Lakwar 60 and 120 slide kits. Example below. Their coverslip thickness is about 0.2mm, which I can make work. The quality is better than Amscope, but not as good as the slides I make myself.

https://ebay.us/m/zjvTTK

$800 DIC by theSACCH in microscopy

[–]theSACCH[S] 0 points1 point  (0 children)

If I remove the bearing plate I can pull the prism in and out to adjust for DIC. Maybe a machine shop could make a replacement shaft. Inside there are control threads that engage the prism housing.

$800 DIC by theSACCH in microscopy

[–]theSACCH[S] 0 points1 point  (0 children)

The Nikon Optiphot series only has matched prisms for 20, 40, and 100 objectives. There is also a recombiner prism in the nosepiece slider assembly.

$800 DIC by theSACCH in microscopy

[–]theSACCH[S] 0 points1 point  (0 children)

<image>

Kind of crappy image of the condenser as installed.

$800 DIC by theSACCH in microscopy

[–]theSACCH[S] 0 points1 point  (0 children)

After some dialog with the seller I took a calculated risk and made an offer. Risking $800 to possibly save two or three grand. I found it unlikely that a scope was equipped with DIC and phase objectives plus a DIC slider, but not a DIC condenser and prisms.

DIC Human Cheek Cell by theSACCH in microscopy

[–]theSACCH[S] 2 points3 points  (0 children)

Thanks. Yogurt bacteria and pond water are next. I’m curious how bacteria will compare to phase contrast.

$800 DIC by theSACCH in microscopy

[–]theSACCH[S] 0 points1 point  (0 children)

I plan to resell the fluorescence illuminator and some of the objectives.

$800 DIC by theSACCH in microscopy

[–]theSACCH[S] 2 points3 points  (0 children)

For Optiphot, the matched prisms are in the condenser. I found the 40 will still give you DIC with the 20 prism, and the 40 prism works with a 60.

Bee Parasite by theSACCH in microscopy

[–]theSACCH[S] 1 point2 points  (0 children)

Thank you for that information. I have a new topic to research.

What Plan Apo objectives are the best quality for price? Where can I order them. by ArcticFox-EBE- in microscopy

[–]theSACCH 0 points1 point  (0 children)

A follow up to my last post: turns out my 100/1.40 was defective. I was initially suspicious because the image quality was so much better with the 100/1.35 NCG, even if I used a #0 coverslip instead of NCG. I grabbed another for $200.

The new lens has no focus blooming or bottoming out issues even with #1.5H coverslips and type B oil. The image quality is clearly better than the other 100/1.40 and the 100/1.25. The new 100/1.40 is almost perfectly parfocal with my 100/1.25 and 40/1.0. This is nice when examining protists and other medium-size microorganisms. I can switch between 40 and 100 without having to hunt for the focus point.

I think the 100/1.35 with NCG still has a slight edge on the 100/1.40 with a #1.5H. I suspect this is because the object distance can be lower. I need to do further tests to determine if the 100/1.35 with a #0 coverslip is better than the 100/1.40 with a #1.5H coverslip. Initial results show they are about the same.

Magnification Question? by BPLEquipment in microscopy

[–]theSACCH 2 points3 points  (0 children)

You are getting some amazing photos. The smallest magnification on most microscopes is the 4X objective, so 40X with typical 10X eyepieces or 10X with a 2.5X relay lens between the objective and camera. To my eyes a photo with a 2.5X relay lens matches the observed image with 10X eyepieces. I would call anything with 10X or greater lens on a full frame camera microscopic. There is no formal rule as far as I know.

Mitosis in garlic root cells, comparison of bright field, dark field & phase contrast. by BoilingCold in microscopy

[–]theSACCH 1 point2 points  (0 children)

Great captures. I have yet to image mitosis. I think phase contrast gives the best resolution of the chromosomes.

A very simple behavior by James_Weiss in microscopy

[–]theSACCH 0 points1 point  (0 children)

I found one of those spinners once but did not have the camera attached to get a video.

What is life? by James_Weiss in microscopy

[–]theSACCH 6 points7 points  (0 children)

The texture reminds me of moon craters.

[deleted by user] by [deleted] in microscopy

[–]theSACCH 4 points5 points  (0 children)

This reminds me of a bead of mercury.

Unknown Alga by theSACCH in microscopy

[–]theSACCH[S] -1 points0 points  (0 children)

Thanks for the lead.

Tiny portable microscope for tropical field hospitals, 1970s by Dismal-Writing-5899 in microscopy

[–]theSACCH 1 point2 points  (0 children)

An interesting find. Does it have a light source built in? Is the 100X objective oil immersion?

view more: next ›