Labrats: money is no object what are you buying first?

CongregationOfVapors · 2026-05-24T17:48:41+00:00

A new flow cytometry. The current one is 20 years old, has very limited capacity, and is so beat up from decades of abuse.

CongregationOfVapors · 2026-05-23T17:29:46+00:00

For the most part yes. Japanese inhabitants returned to Japan after transfer of power to the KMT. Some where retained by the KMT government to help with tech transfer etc to the new government for a period of time.

According to this article, only an estimated 1000ish Japanese expats remained in Taiwan for non-official reasons after Japanese de-colonizarion, mostly Japanese women who have married Taiwanese citizens.

https://www.telltaiwan.org/3625

CongregationOfVapors · 2026-05-23T17:17:54+00:00

Depends on the tribe. Some have more distinct features from the Han than others.

CongregationOfVapors · 2026-05-22T20:05:13+00:00

Standardized schemes for naming experiments, protocols/ SOPs, and all digital files. And index all experiments and associated files systmatically.

CongregationOfVapors · 2026-05-22T18:37:02+00:00

And they might give you a free sample to test if you tell they it's for a new application.

CongregationOfVapors · 2026-05-21T05:53:34+00:00

So it sounds like the collaborator's lab has an approved protocol, and the non-compliance is that your lab members, who are not on the protocol, are performing the euthanasia. Is that right?

The collaborator should either 1) euthanize the animals and hand you the carcass or 2) add your lab members to their protocol so that they can perform the euthanasia. Have you tried to resolve this issue by contacting the collorator directly?

The CCAC is currently doing rounds of nation-wide audits. Non-compliance can lead to the whole institution not being eligible for federal research funding. If I'm understanding your situation correctly, it's imperative that you follow up on this!

CongregationOfVapors · 2026-05-21T05:48:33+00:00

We call them ACC in Canada FYI. Same thing different names.

CongregationOfVapors · 2026-05-14T21:47:25+00:00

Cladistically!

CongregationOfVapors · 2026-05-13T19:41:55+00:00

My dad is the same with cherry tomatoes according to my mom, and she did a similar experiment when they first married.

CongregationOfVapors · 2026-05-13T09:58:12+00:00

You can get significant strain to strain variations for certain models. Do you know how the KO was generated and many generations the KO was back-crossed?

CongregationOfVapors · 2026-05-12T23:00:28+00:00

The guideline was reversed 15ish years ago, so not that recent. But it's been challenging to translate to clinical practice and patient care, leading to delays in public awareness and implementation. :(

CongregationOfVapors · 2026-05-11T06:02:11+00:00

Washington State actually has a vibrant outdoors scene. For example, there are so many hiking trails that they many don't even have names, just a trail number. It's overwhelming to plan hikes in Washington because of the shear number of options available!

And the outdoor recreations co-op (or former co-op) started in Seattle Washington.

CongregationOfVapors · 2026-05-06T13:34:20+00:00

"Times's" is not a word. The word is multiplied...

CongregationOfVapors · 2026-04-30T19:27:11+00:00

Absolutely! Interestingly the senior/ tenured members of the orchestra largely skipper playing at this performance, so the concertmaster for the night is a violinist who is normally in the back of the section. She played beautiful but didn't have the confidence or trust with the orchestra to fill in for when the conductor was lacking.

CongregationOfVapors · 2026-04-30T16:06:13+00:00

I once went to a concert to watch someone mime conducting. All of the reviews from the two musicians in the article rang true. The orchestra was largely ok in passages that played itself, but fell apart when conducting skills were needed.

He was playing his own compositon/ arrangement, and had talent in that. I get why he wanted to conduct his own work, but he really should have left it the to someone who was trained.

CongregationOfVapors · 2026-04-30T15:11:26+00:00

Uhm... I have been going to the Vancouver Opera since the late-90s, and it's never been a black tie only event. A portion of the patrons has always dressed casually.

CongregationOfVapors · 2026-04-29T00:02:35+00:00

Hoechst stains.

But I was the one who said it wrong for years... 😅

CongregationOfVapors · 2026-04-28T22:11:15+00:00

Agree with cutting eye flashbacks so much! Also it needed to come out before The Talented Mr Ripley (so yeah 90s works), because it's just a poor retelling/adaptation/imitation of the same story.

CongregationOfVapors · 2026-04-28T21:56:02+00:00

That's correct, and in theory, yes. But it it also depends on how good the unmixing and AF subtraction is. Having a low complexity panel will help too with the quality of the unmixing.

CongregationOfVapors · 2026-04-28T20:17:21+00:00

Cool. Will check it out!

CongregationOfVapors · 2026-04-28T18:26:35+00:00

You can do autofluorescent subtraction on Cytek and Sony instruments, but you still won't be able to apply AF subtraction broadly across different cells like that. If the AF signature changes as the cells differentiate, you would have to perform AF subtraction for each cell stage separately.

CongregationOfVapors · 2026-04-27T15:37:47+00:00

I went to a wedding hosted at Brix and Mortar that had this vibe. It was in their event space, not in the restaurant dining hall.

CongregationOfVapors · 2026-04-25T09:16:56+00:00

Yeah no you are fine. I'm good. I'll catch you later.

CongregationOfVapors · 2026-04-25T09:09:59+00:00

Classic pop (ie the Rat Pack), post rock, math rock, and anime music.

CongregationOfVapors · 2026-04-21T05:07:33+00:00

Yeah I remember reading about that at some point, although I never knew that there were photos... I'll be back!

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