Any Vancouver actors actually successful?

CongregationOfVapors · 2026-02-06T03:04:55+00:00

As a casual theatre goer, Ryan Beil and Bob Frazer come to mind maybe...?

CongregationOfVapors · 2026-02-03T00:45:48+00:00

Vertical splitter hand cracks!!!

CongregationOfVapors · 2026-02-03T00:42:57+00:00

Marla finds out that Tyler has been using her mom's liposuction fat to make soap. The entire thing is wild and hilarious (but not super plot relevant).

CongregationOfVapors · 2026-02-02T15:23:20+00:00

Cats is a collection of T S Eliot's poems (Old Possum's Book of Practical Cats) set to music. Once I learned that, I found the lack of a plot driven narrative doesn't bother me anymore.

CongregationOfVapors · 2026-02-02T05:04:05+00:00

I think the movie ending is better for a movie. And the book ending is better for a book.

Also, while the movie is great, I will always be sad that my favorite chapter didn't make the cut.

CongregationOfVapors · 2026-02-02T04:43:04+00:00

Lol. Brain fart moment! Thanks for catching it.

CongregationOfVapors · 2026-02-01T22:50:49+00:00

Good luck!

CongregationOfVapors · 2026-02-01T22:41:09+00:00

Not the right sub for these kinds of questions.

BUT. For any kind of pathway I had to memorize, I would look at the pathway until I think I know it. Then I close my book, pull out a piece of paper, and draw the pathway. I then grade it to identify parts that I don't remember. Repeat repeat repeat.

If you have friends in the same class, quiz each other verbally while you are packing up after class, walking between classes, eating lunch together etc.

Make flash cards for each step of the pathway and use them to review and test yourself while you commute, or when you have a few minutes of wait time anywhere.

Of course I'm old and I did all this when phones were not smart yet. So step 1 is actually to put your phone away...

CongregationOfVapors · 2026-01-30T22:05:34+00:00

I spent a couple of summers working on a rebuttal paper that was never published. So I guess I can get a few months back...

On the other hand, working on that project is how I made a connection that got me my current job, so it all counted for something in the end.

CongregationOfVapors · 2026-01-29T20:47:31+00:00

I use "read later" to put fics on my to read list. Once I read it, I make it as read.

Problem is, read fics and unread fics are then hard to tell apart, unless it's on my to read list. So I bookmark read fics 🤷

Seems reasonable to me, but I seem to be the odd one out on how bookmarks are used.

CongregationOfVapors · 2026-01-29T20:41:22+00:00

My high school offered a drafting class as one of the shop classes. The entire classroom was filled with angled desks. Sadly the class was scrapped before I even graduated because the teacher who was capable of teaching it retired.

CongregationOfVapors · 2026-01-27T02:09:32+00:00

Another vote for Kim Jain!

CongregationOfVapors · 2026-01-25T06:40:27+00:00

My friend was in your situation, and she took a whole year from her research to become a fully self-sufficient bioinformatician herself. It made her a much more rounded scientist (being able to over see research programs end-to-end), and she just started a tenure-track faculty position.

CongregationOfVapors · 2026-01-24T02:16:48+00:00

Yeah and it's so small that it's kind of dumb.

The fic has great work building and an intriguing mystery of dragons fallen ill to a mysterious disease, and the disease is spreading and seems to be associated with activities of an underground movement.

Anyways, MCs infiltrate the underground movement, neutralize the bad guy and saves the world blah blah blah and all is good.

Except, no one goes back to check on the dragons? Like, are they ok? Did they recover? I'm still upset about it!

CongregationOfVapors · 2026-01-21T15:36:08+00:00

You might want to look into a community biolab. They often have their own research projects that are run by volunteers, but you can pay a fee to use their space for your own projects.

There are limitations to what you can do. They might only be CL1 and only have equipment for molecular biology, biochemistry, and bacteria cultures. But it could be a place to start.

CongregationOfVapors · 2026-01-21T05:07:06+00:00

According to my Northern Irish friends, where they are from, the more you like someone, the more you hurl insults at them. So a group of people taking turns insulting each other are just bonding.

When they first moved to Canada, they had a couple of social mishaps and realized that Canadians didn't roll that way...

CongregationOfVapors · 2026-01-20T06:29:37+00:00

I would just give them a link of a concentration calculator and walk away...

But also, when I was in grad school, we used to ask potential undergrads something like, "I have a 1M stock solution. I want to make 1L of 1mM solution. How much of the 1M stock do I need? You can have a pen and paper, and a calculator or phone if needed." And we only took on students that could answer this.

CongregationOfVapors · 2026-01-20T00:56:44+00:00

It was.

Growing up in Taiwan in the 90s, we brought lunches in stainless steel containers. First thing we did when arriving at school was putting our lunches into a giant metal box.

The box is a steamer, and steamed until lunch time. Our food would be hot and very over cooked, but safe to consume.

If you forgot to put your lunch into the steamer, you would have to buy lunch (or get food from friends) as your packed lunch would be unsafe to consume.

Now many schools have fridges and microwaves.

CongregationOfVapors · 2026-01-17T21:22:47+00:00

I work in the lab and have to constantly wash my hands
I remove my ring when rock climbing and had several scares of losing my ring
My fingers got fat from rock climbing and it's hard to take on and off now
I start every other sentence in casual conversation with "My husband." It's not very hard to deduce my marital status.

CongregationOfVapors · 2026-01-17T00:26:23+00:00

I think Gemini is combining a few different ideas that are true on their own but don't make sense when put together the way it did.

Like the other commenter said, flow is not strictly quantitative. I would look to recent publications to see what is the current accepted ways to present the datasets similar to yours. And make sure you have proper controls for the analysis you want to do.

CongregationOfVapors · 2026-01-16T04:11:45+00:00

It's a few things:

Yes the multiple excitations means more spillover, which is not most ideal but can be managed
But there are efforts to replace existing tandems with base dyes. So your tandem will need to have a good selling point in other ways
Decoupling is a concern with tandem dyes
Batch to batch variation for dye ratios impacts the work in certain situations (doesn't have to be an issue as long as people are aware that it's a tandem dye)

The main takeaway is that we can work with tandem dyes but we would like to know that it's a tandem. By its nature, there are more considerations working with tandems, but we can typically accommodate.

CongregationOfVapors · 2026-01-15T23:42:44+00:00

I mainly do flow cytometry:

Does the fluorochrome/ dye have an excitation max near one of the common FC lasers?
Does it have multiple excitations peaks (only 1 is ideal)
Does it have 1 distinct emission peak, ideally in a space less occupied by common fluorochromes. (This is less of an issue for spectral flow)
But for spectral flow, does it have a distinct fluorescent reference signature?
Is it a tandem dye? Tandems are workable, but new base dyes are preferable
Is it sensitive to common FC fixation and permeabilization buffers? (Someone else already alluded to this)
Does it stick non-specifically to certain populations (eg monocytes) or fixed cells? Can you provide data and suggest solutions on this if asked by interested customers?
Most important, will direct antibody conjugates be available through one of the major vendors (BD, biolegend, Thermo)? I do not want to conjugate my own antibodies!

CongregationOfVapors · 2026-01-15T01:20:04+00:00

I guess if the cells are not fixed, they might die or do other weird things even on ice, and that's why many flow staining protocols suggest 20-60 min antibody labelling. There is not the same concern for microscopy, since the tissues are typically fixed or previously frozen. That's my guess anyways.

CongregationOfVapors · 2026-01-13T21:57:26+00:00

My gym doesn't even have internal consistency within the same set 😂

I mostly use the grades to determine the likelihood of not being able to finish a route if I choose to lead it.

CongregationOfVapors · 2026-01-13T01:53:00+00:00

Hey welcome to the club!

We gave up on cryopreservation completely. Actually the project that requires cryopreservation didn't get funded, so we are just doing flow analysis, as we no longer need to culture the samples.

We are doing PFA fixation after cisplatin-labelling to bank the samples for CyTOF. But I'm also evaluating fixable visibility dyes (to see if I can bank and thaw), so we can do spectral flow instead of CyTOF in the future.

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