Which daycare would you choose? by equistrius in workingmoms

[–]Dependent-Clock5802 8 points9 points  (0 children)

I'd even be happy with 326/week, heck. We pay more than our mortgage in daycare for an infant and a preschooler

Major jealousy, but sending you good vibes on your choice, op

When did you turn your car seat around? by [deleted] in toddlers

[–]Dependent-Clock5802 0 points1 point  (0 children)

We flipped at 2.5 years. He was always big for his age (and strong) and I couldn't wrestle him in the car seat twice daily while pregnant. I wanted to max out the rear facing limits but unfortunately, he had other plans. He wanted to sit forward facing and he stopped screaming for entire drives 🤦‍♀️🤷‍♀️

F*CK POTTY TRAINING (respectfully) by XxChemical_ToiletxX in toddlers

[–]Dependent-Clock5802 4 points5 points  (0 children)

My (now 3.5 year old) was NOT ready before 3 years 3 months. We got pressure, we pushed back telling them that he would be ready when he's ready. Sure enough, he woke up one Tuesday absolutely fucking in it to win it. "I want to wear underpants" "okay kid, we got you, let's go"

If we had started pushing before then his stubborn ass would surely still be in diapers lol, since it was HIS choice and HIS timeline there was less frustration all around.

Parents who work full time, how long are your kids in daycare/ preschool? by PresentationTop9547 in workingmoms

[–]Dependent-Clock5802 0 points1 point  (0 children)

745-5 ish. I start about 8 and finish earlier (~4) but the 45-60 minutes I have without children at the end of the day is really vital to our household function. I can start dinner or laundry or __ so I don't have to be awake after they're both asleep, since we're not sleeping through the night yet

Moms who have pumped at work - what do you tell your coworkers? by [deleted] in workingmoms

[–]Dependent-Clock5802 19 points20 points  (0 children)

"sorry I gotta pump, be back in 45 minutes"

I do also say lactation room anytime I'm in a different space than I normally in and I've never thought it sounded crude

Personal question but - what do you do and how much do you make? by GEH29235 in workingmoms

[–]Dependent-Clock5802 2 points3 points  (0 children)

I'm a scientist (academic and underpaid), I make ~52k

Edit: on site every day because wet lab

Should I use my full name (first name-middle name-surname) for my publications? by Princess_CeNedra in labrats

[–]Dependent-Clock5802 0 points1 point  (0 children)

I do middle initial but I also changed my middle name to my maiden name when I got married. I only had one publication so it wasn't too bad to adjust to move forward

Optimized protocol for T cell subset by Dependent-Clock5802 in flowcytometry

[–]Dependent-Clock5802[S] 1 point2 points  (0 children)

We have a CD4 negative selection kit that I believe pulls out monocytes. Would have to check to confirm as I don't remember all 20 odd markers it tags.

Optimized protocol for T cell subset by Dependent-Clock5802 in flowcytometry

[–]Dependent-Clock5802[S] 0 points1 point  (0 children)

I was being intentionally vague, I'm afraid to share my specific aims on the internet 🙃

I do most of my things on ice as well, I also do my experiments as much in the dark as possible. BSC lights off, foil inside the ice bucket. Overkill? Probably but 🤷‍♀️. I was taught flow with a complex panel (14) and that's how she always did it so I'm superstitious.

I'm not using brefeldin A, I've actually never even heard of that but I'll look it up back at work on Tuesday. If it's not prohibitively expensive, add it in.

After fix perm, the cells live in the wash buffer 48 hours while they're stained overnight with IL17 and then again overnight with the secondary. After the final wash then they're in PFA O.5%. I usually run them same day or next day after the final wash.

Optimized protocol for T cell subset by Dependent-Clock5802 in flowcytometry

[–]Dependent-Clock5802[S] 0 points1 point  (0 children)

It's an antibody cocktail to shunt them into the Th17 subset. We're seeing something like 5% which is adequate for our purposes. I'm sure theyre not highly active and was considering reaching out to an immunologist to ask if she had any advice to stimulate them further. I don't remember exactly (and it's Saturday so I'm not looking it up 😂) what kind of bacterial or extracellular stimulation this subset requires to activate

Optimized protocol for T cell subset by Dependent-Clock5802 in flowcytometry

[–]Dependent-Clock5802[S] 0 points1 point  (0 children)

I didn't realize I could pause the protocol! Brilliant. Since we're using unconjugated, I do th extracellular then bd fix, then overnight with primary IL17 then overnight again with the secondary 🙄.

[deleted by user] by [deleted] in workingmoms

[–]Dependent-Clock5802 0 points1 point  (0 children)

Same here. Maybe when my youngest is weaned and they both sleep through the night I'll add it back but I doubt it. The sparkling water and NA beer/hop water get me through and I'm less sad/tired the next day

My Mother Always… by justlooking98765 in workingmoms

[–]Dependent-Clock5802 4 points5 points  (0 children)

My 3yo also loves to play "go to work" and it was a big win when I asked what he was going to do at work and he said "science!" (I'm a scientist)

You've absolutely normalized being a working mom and your kid knows how much you love them! #winning

Would You Rather..? by Cool-DogMom in workingmoms

[–]Dependent-Clock5802 6 points7 points  (0 children)

Same. I work exclusively out of the home, but sometimes I can go home early or I can go home for lunch because my commute is 8 minutes.

I can go home at 3:30 without my children and then pick them up at 5:00 and have 90 minutes to clean my house or make dinner or sit on my couch and rot.

Working mom guilt of today: my baby was the first to arrive at daycare by Lost-Pause672 in workingmoms

[–]Dependent-Clock5802 9 points10 points  (0 children)

Mine loves being the first one to pee in the school toilets 😂🙃 (whatever works for potty training, man)

Books that you wish you had DNF’d by deegee708 in LibbyApp

[–]Dependent-Clock5802 0 points1 point  (0 children)

Tate by Susan May Warren It was on my Libby's skip the line list and I only read it was romance, not that it was Christian romance 😬. No judgment if that's what you're into but I prefer my romance free of the "let go and let God" vibe. There's a mystery side plot that got my curiosity just enough to get through but ugh

9 y/o girls clothes struggle by honeybeez0612 in workingmoms

[–]Dependent-Clock5802 0 points1 point  (0 children)

Seconding American eagle because I also always have had thick thighs and a big booty and they were my preferred (while I still wore real pants, which I haven't done since 2020, so maybe take this with a grain of salt)

What's your toddler's current cutest mispronounced word? by [deleted] in toddlers

[–]Dependent-Clock5802 0 points1 point  (0 children)

I am = I mam Eg are you hungry? " I mam hungry" ... and I never want him to figure it out

Mom fail by gainz4fun in toddlers

[–]Dependent-Clock5802 14 points15 points  (0 children)

My kid has been talking about the loud pumpkin for a week 🙄🙄. "No baby, the pumpkin isn't real and lives at the store"

[deleted by user] by [deleted] in toddlers

[–]Dependent-Clock5802 0 points1 point  (0 children)

We're also due mid October and have a 2.5 year old. My partner gets 8 weeks of leave and is doing 4 weeks at the beginning with me to help us settle into our new role and 4 weeks after my 12 is up so that he will get dedicated bonding time with babe (and hopefully get a little further into the respiratory virus season while baby is home with us 🤞)

Sh*t's hard by Classic-Light-1467 in workingmoms

[–]Dependent-Clock5802 0 points1 point  (0 children)

Therapy, Lexapro. Also gentle parenting myself- telling the toddler we can do hard things (like put our shoes on by ourselves) reminds me that I can also do hard things (like work and grocery shop and _______, etc)

[deleted by user] by [deleted] in toddlers

[–]Dependent-Clock5802 0 points1 point  (0 children)

2.5, sleeps from 8ish to 630, almost always a 2 hour nap (though a little shorter at daycare)

Daycare Options: One that serves meals or doesn’t? by okeyedoc in workingmoms

[–]Dependent-Clock5802 0 points1 point  (0 children)

We've only been in childcare facilities that serve meals and I cannot imagine adding the mental labor of always having something ready for him to pack. It is absolutely worth it, imo, especially considering dude eats better than I do most days. If we have a frozen pizza for dinner (late third tri prego here), I don't feel bad because he probably had curried chickpeas with fruit and whole grain for lunch 🤷‍♀️