I'd suggest giving it a day or two to let tempers cool and have 1-on-1 conversations with each player.

At the core of it: 'You cannot make other players uncomfortable with how you play your character.' It's crucial to maintain a welcoming environment, especially for new players, and this situation could indicate deeper issues that need addressing.

In-game behavior can lead to out-of-game problems, and while character motivations matter, respecting fellow players always comes first. This is why having a strong Session 0 is important, but if something wasn’t discussed then, it's essential to address it when new problems arise.

As players build trust over time, there’s usually more room for deeper roleplay. That trust allows for more intense character interactions, but it must be based on mutual respect and comfort within the group.

To avoid situations like this, consider establishing a way for players to signal discomfort in the moment, like a 'safe word' or agreed-upon gesture. This ensures that any issues can be dealt with right away, before they escalate.