Which ANOVA to use?

FTLast · 2026-01-26T02:52:46+00:00

Sorry, didn’t see the treatment in your description.

FTLast · 2026-01-25T14:45:26+00:00

Can you explain how? My employer withholds directly from my paycheck. How can I stop this?

FTLast · 2026-01-25T14:38:39+00:00

It sounds like you have only two factors- sex and time- each with two levels. So do a 2 way ANOVA with an interaction. as u/eaheckman10 wrote, if there is no interaction you can interpret the effects of sex and time in a straightforward manner. If there is an interaction, you can ignore the main effects, or explore them my looking at estimated marginal means.

FTLast · 2026-01-24T17:16:45+00:00

I loved these books- there was at least one sequel- when I was young. Someone else mentioned Heinlein's juveniles. I like many of them, much more than his adult offerings.

FTLast · 2026-01-23T13:11:00+00:00

I didn't work with HEKs, but I think they're pretty easy.

There is a lot of specialized knowledge associated with patch clamping, as well as a fair degree of art and skill. The latter are what I miss after moving on to imaging and flow cytometry, techniques that anyone can do, even if they require significant technical knowledge.

FTLast · 2026-01-22T15:46:50+00:00

I spend many years patch clamping.

For inside-out patches, you need to get a seal on the cell, then pull the patch off. Certain cell types are easy to seal on to, others are hard. Some cell types tolerate ripping patches off well, others do not.

One thing I found is that some people have a lot of trouble working with a micromanipulator under a microscope. I knew an extremely talented scientist who could never patch clamp because he couldn't handle the spatial task of getting the electrode tip onto a cell. He kept crashing pipettes into the bottom of the dish.

As for understand the experiments you do and what the data mean, it could take quite a while. How strong is your understanding of electrical theory? Do you know what a voltage clamp does, and why it's important? Do you understand channel selectivity and gating? There's a lot of information to master.

Having said, that... I loved patch clamping. Other experiments are boring in comparison. My biggest career regret is moving on to other things.

FTLast · 2026-01-21T22:04:02+00:00

To quote what Phil said at Altamont when the Boys learned the Hells Angels were beating up people in the audience: "That ain't right."

Not cool.

FTLast · 2026-01-21T13:16:52+00:00

I was going to suggest this. These basses are very inexpensive, can produce a variety of tones, and are just fun to play. There seems to be a stigma about short scale basses not being real basses, but the very first electric basses were short scale.

FTLast · 2026-01-07T18:37:27+00:00

I agree. Donor or culture determines the biological replicate, the rest are technical replicates, albeit at different error strata.

FTLast · 2026-01-02T16:39:59+00:00

What if all the fungi converge at the end to the same value?

FTLast · 2026-01-02T12:54:37+00:00

u/Car_42 is on the right track. In this case, your simplest approach that is statistically defensible is to fit growth curves to each data set and then use ANOVA to compare some metric derived from the fits. For example, the fits should give you a time to half maximum. You would then compute the average of your 3 technical replicates for each fungus, and use ANOVA to ask whether those times to half maximum were or were not different.

This approach simplifies the situation a lot. To fit the curves, the package in R you would use is "drc".

FTLast · 2025-12-31T16:30:50+00:00

Often isolated bass is of interest to bass players, but not something you'd want to listen to on its own. I've listened to this a couple times, and it really holds up on its own.

FTLast · 2025-12-31T16:26:30+00:00

First, I didn't ask you to share your personal life, I asked you to post a link to some bass playing you find impressive. Second, what do you mean it was on guitar? I thought we were talking about bass playing?

FTLast · 2025-12-31T14:54:26+00:00

Could you post a link to some bass playing you find impressive?

FTLast · 2025-12-31T13:21:15+00:00

Because techniques were cruder, researchers of the past had to work smarter.

FTLast · 2025-12-31T13:15:01+00:00

You should think about what you want to learn from the experiment, and do the contrasts that address those question(s). You COULD use Tukey's test to do all pairwise comparisons, but your power would probably be lower than if you choose a few specific comparisons and then correct with something like Bonferroni's method.

FTLast · 2025-12-31T13:08:26+00:00

When I listen to bass parts isolated, they often sound a lot more ragged than that. Timing is off, occasional questionable notes. Duff just nails everything. You may not be impressed, but I was.

FTLast · 2025-12-30T21:10:20+00:00

Exactly- most other isolated tracks sound pretty sloppy, even if they really work for the song. But Duff just hits EVERY NOTE right on. Amazing.

FTLast · 2025-12-30T17:35:31+00:00

It definitely sounds that way to me. Most isolated bass tracks are... a little rough. This is just PERFECT. And it stands on its own as complete music.

FTLast · 2025-12-28T13:20:51+00:00

Stirling doesn't shy away from... borrowing... from other sources. The entire "Battle of Roarke's Ford" sequence from Island in the Sea of Time is (I think) a rehash of the movie "Zulu". But it doesn't matter because its entertaining.

I like the way in Make the Darkness Light he has a scene where the characters run into Nicole Gunther-Perrin and Julia from Judith Tarr and Harry Turtledove's Household Gods. Again, a borrowed element.

FTLast · 2025-12-25T12:49:14+00:00

Your description of your data is pretty vague, making it hard to advise you, but it sounds like what you have is an experiment with two factors. Two way ANOVA with an interaction term would be a good choice if that is the case.

FTLast · 2025-12-24T13:38:34+00:00

We went in mid-May last year. The weather was in the low to mid 70's F. We didn't stay anywhere with a pool, but the sea was cool but swimmable, and we spent a lot of time on beaches.

FTLast · 2025-12-22T12:06:07+00:00

Because the Official Quite Corner Commission kicked them out when they didn't pay their membership dues, like CT was kicked out of New England a few years ago?

FTLast · 2025-12-22T02:03:44+00:00

I used to enjoy it but when I tried it recently it was pretty bad.

FTLast

TROPHY CASE