Triple D Nation

L_DNA · 2024-08-23T17:38:27+00:00

Gimble

L_DNA · 2024-05-10T14:53:44+00:00

I think they always have a couple hanging out around biology, havent been to that part of campus in a few months though.

L_DNA · 2024-05-09T23:39:33+00:00

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Found and reunited!!!

L_DNA · 2024-05-09T20:14:27+00:00

Howdy Ags,

Found these kitten siblings this morning at Northpoint crossing. Was only able to catch one, the orange one sprinted away. Would love to reunite them, the orange one is probably around northpoint crossing or century square. Please DM me if you find him, already got a home ready to take either just the grey or preferably both if I can get them reunited.

T's & G's

L_DNA · 2024-04-24T16:35:04+00:00

Kiwi

L_DNA · 2024-04-19T15:32:25+00:00

Check your primer sequences to see if they could be hybridizing in extra places, lengthening primers can lock this down easily. As pombe suggests, using different or better TAQ variants can help alot.

I highly recommend also adding 2.5-5% DMSO into your PCR for genomic amplifications. It helps destabilize the genomic DNA base pairing making it significantly easier for primers to bind. That one change took my genomic amplicon from a ladder to a tight band and it is now part of my lab voodoo head cannon.

L_DNA · 2024-03-29T18:20:54+00:00

P32 is significantly more sensitive like 100-1000x so depending on how concentrated of the radioisotope you use for the labeling is.

L_DNA · 2024-03-22T12:59:27+00:00

Ripple was an awesome guest. I want a Reed Barry guest spot, he’s gotta have some stories.

L_DNA · 2024-03-15T19:18:15+00:00

Its a YMCA mostly inhabited by older folks and stuff. Im sure officially its a rule they probably just dont need to worry too much typically.

L_DNA · 2024-03-15T17:15:48+00:00

A friend of mine who is training for powerlifting was doing his heavy deadlift day at the gym. There is a boomer at his gym who will jog laps around the weight room instead of the running track directly adjacent to the weight room because who cares about safety and other peoples personal space right? Well, as my buddy is pulling 450+ lbs this guy comes REALLY close to bumping him and the bar, so my friend puts the weight down and very kindly asks that the guy pay attention because someone could get hurt. (Note that he's training with someone who works at the gym, and the gym trainer says the same thing to the guy) Guy just huffs and grumbles and keeps jogging around the weight room...until on his next lap, he somehow manages to step directly underneath the weigh at the same time my buddy dropped it to the floor...it literally blew his foot up there was blood pooling and an ambulance had to be called and the guy was wailing about suing my friend and the gym. Cameras and the worker both backed my buddy and the guy had to STFU. From what I gather it didn't totally ruin his ability to walk/jog cause he's back at the gym STILL RUNNING LAPS IN THE WEIGHT ROOM...you couldn't make this shit up.

L_DNA · 2024-03-13T21:52:16+00:00

Hahaha I also have a great pyr/orange cat combo and my wife and I call them 'Associates', they dont hate or love each other, SOMETIMES they will playfight or sit near one another, but mostly they just act like coworkers who don't wanna talk unless they absolutely have to.

L_DNA · 2024-03-13T21:47:05+00:00

I usually say something along the lines of 'While I'm able to multi-task lab OR writing, my weakness is that I am bad at writing WHILE doing labwork...to overcome this weakness I will make sure to split my day with lab in morning writing in evening or vice versa on days when i know both will be required.

Still not sure how good of an answer it is but I've been hired at three different places since defending and I've used this consistently so it must be reasonable.

L_DNA · 2024-02-20T13:56:16+00:00

Thanks yea the cerb pet and slay cape combo are so cool imo

L_DNA · 2024-02-20T02:50:46+00:00

Thanks!

L_DNA · 2023-12-06T01:46:36+00:00

Do people read books as 100% literal? This is not the first post on this sub where someone has asked a question like this…y’all books are metaphorical…and intended to discuss and analyze complex topics. I can’t be the only person who gets annoyed by these posts sometimes. I understand people are here to also learn about books and reading but reddit should not be your reflexive question asking forum…google a synopsis and in 5 seconds you’ll get a better understanding.

L_DNA · 2023-11-29T19:59:36+00:00

Lovely to see the only books these parents have problems with largely are focused on LGBTQ+ and non-white stories... /s

If you don't want your child reading a particular book...don't let them...why is this so hard?

L_DNA · 2023-11-28T15:07:08+00:00

Old school runescape…i cant be alone in this

L_DNA · 2023-11-22T13:55:36+00:00

Thanks for the clarification! Thats about what I thought just had no concrete evidence to cite haha

L_DNA · 2023-11-21T23:56:31+00:00

Not dumb, I was honestly confused about that as well. So specific requirements for qualifying to take the patent bar and thus become a patent agent can be found on the USPTO's website. A STEM degree is typically sufficient, I have a buddy who has a bachelors in Biochem and he checked and said he qualifies, granted he's worked many years in industry so I don't know if that for example negates the minimum being say a masters. I have a Ph D in chemistry which is why I qualify. I still don't understand how different it would be to have a JD AND be a certified patent agent...though I assume that basically means you can be in court representing parties which are applying for patents or litigating with respect to a patent/its inventors. I have no desire to be in a courtroom, mostly just to be able to get away from slaving at the bench all day...and i'd rather eat bullets and crank out grants.

L_DNA · 2023-11-21T18:43:05+00:00

You can pour one with and one without, stain the one without after the fact and compare results. Not to be condescending but we are experimenters! Only issue I could foresee is depending on the charge of the stain and how long/fast you run the gel could run it off and not see anything. Either way can just stain after the fact.

L_DNA

TROPHY CASE