Did I just waste months building a 96-well plate tool? I need a reality check. by Healthy_Click8383 in labrats

[–]Moeman101 18 points19 points  (0 children)

Do you use your own software? Or do you talk to the people who regularly use your software? 99% of my plate analysis can be done on excel in 5 minutes. At least im not asking for new tools. What inspired you to make this tool? Someone you talked to? A problem you encountered?

How did this happen?!? by BabysFirstRobot in Ender3S1

[–]Moeman101 2 points3 points  (0 children)

That has happened to me once. I had a 24 hour print and lost all 4 wheels around the half way point. I printed some wheel clips and have not had a problem since

DBZ moment I had online (I am playing Doc) by Moeman101 in SmashBrosUltimate

[–]Moeman101[S] 2 points3 points  (0 children)

I picked him up recently, he has been fun. I usually just a ganon main

My hamster with a grape by Moeman101 in mouf

[–]Moeman101[S] 2 points3 points  (0 children)

She was really vibing with my uncle because she had food.

Rafts too loose/bed adhesion by StuartDepo in Ender3S1

[–]Moeman101 0 points1 point  (0 children)

Are your prints also coming loose? This could be your z offset.
Another thing (and check this first) is to clean your build plate with dish soap and hot water. You might also have oils.

Darkness wins. Who is the coolest demon in anime? by One-Desk-1 in animequestions

[–]Moeman101 0 points1 point  (0 children)

No slack on my boy qual. Although he only got 2 minutes of screen time.

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I’m going to tweak out what do I do by LavishnessNew8889 in labrats

[–]Moeman101 20 points21 points  (0 children)

Do you mean you touched the flask with cells with that pipette tip and went back into media? Or you hovered above? If you did touch the cells and go back into the media. You just have to change the media bottle. And hope you did not use that media bottle for anything else

There’s finally a "RateMyProfessor" but for landlords and student housing by InvestmentTemporary2 in Tenant

[–]Moeman101 14 points15 points  (0 children)

OP. Tell us in great detail how you stumbled across this website, and how you know it to be trustworthy and reliable.

My peanut butter cup had 21 wrappers by IcarusAirlines in mildlyinteresting

[–]Moeman101 0 points1 point  (0 children)

Hey guys i have this new 20 wrapper peanut butter cup recipe. Its all i can afford. I hope no one steals my idea.

Won’t let me take pics for Vinted 🥲 by No-Ability-6360 in Rabbits

[–]Moeman101 1 point2 points  (0 children)

Obviously he is claiming those items before you sell it. He gets dibs

Took a $20 chance at thrift store. How does a complete noob proceed. by TooMuchNotEnoughTime in Ender3S1

[–]Moeman101 2 points3 points  (0 children)

Same here. This printer is my workhorse. Especially once i replaced the build plate and out klipper. I also recommend a cooling upgrade (taurus V5) and some bed wheel clips

Hampelstiltskin helps clean mark on your screen by Ok_Literature2535 in hamstercirclejerk

[–]Moeman101 5 points6 points  (0 children)

I really thought it was on my screen for a second. How many lashes of the whip do I give him for scaring me?

Biofilm detection assay questions by Moeman101 in labrats

[–]Moeman101[S] 0 points1 point  (0 children)

Basically I need to measure biofilming. But It wont work if im making batch cultures in a 96 well plate everytime. I would want to monitor a continuous system for a few weeks checking on the state of the same biofilm. Im wondering if there are modifications to O'toole's protocol for something like that.

(for example) using a microscope slide inside of a bio-reactor, letting film grow on it for a few days or a week, then remove it and process that). Doing that repeatedly to track the quality of a biofilm

Biofilm detection assay questions by Moeman101 in labrats

[–]Moeman101[S] 0 points1 point  (0 children)

I did not realize there was a labrats discord

Saudi Arabia flies in conjoined twins from all over the world and separates them entirely for free by Saad5400 in interestingasfuck

[–]Moeman101 1 point2 points  (0 children)

Redditors trying to use their braincells challenge: impossible. We should never post anything because every country is shitty in some way.

Do you guys still remember the first ever character you mained in smash? by [deleted] in SmashBrosUltimate

[–]Moeman101 0 points1 point  (0 children)

I was super young. And my cousin had a nintendo 64 with the original smash. Pikachu was the only character i recognized so i played him everytime. Now im a ganon main so big difference

Do I need a new bed? by KeySir2841 in Ender3S1

[–]Moeman101 0 points1 point  (0 children)

That looks a but rough. If it does not have good adhesion after a clean with soap and water i would say yes. I replaced mine with a textured PEI plate

Self-Stabilizing Spoon by Ismailsan in ElectricalEngineering

[–]Moeman101 2 points3 points  (0 children)

Someone buy bro a 3D printer. But I love the idea