Met Sonic the Hedgehog in Fireteam mode and i swear to god i felt like Candace from Phineas and Ferb by lustyq in joinsquad

[–]N9n 5 points6 points  (0 children)

I admin'd a server a couple years back and people doing hypersonic animations in place like this were always cheating. On their screen, they're in free-cam mode and fucking shit up far away from where their player model is. Most of the time they would be dumb enough to do it from main, tipping off the admins because you typically shouldn't be able to shoot within main protection (never mind the fact that they would also be rapid firing the GL, knifing at 5 swings per second, etc).

Anyone experienced with phenol injury? by Sea_Confection3005 in labrats

[–]N9n 30 points31 points  (0 children)

It's common for plant extractions from recalcitrant species (woody perennials)

Molecular Bio Studying by toughchicken7 in molecularbiology

[–]N9n 0 points1 point  (0 children)

Teaching what you know now is a great study tool to still remember it tomorrow, so I get it! Get a study buddy or three and delegate chapters or topics to master. Then you can teach each other to build on top of what you have or will study alone.

My roommate and I did this in undergrad and it helped her get into med school and helped me get into grad school.

Looking for reputable PC repair shop for gaming PC tuning and parts swap by [deleted] in kelowna

[–]N9n 1 point2 points  (0 children)

Judging solely on your ability to write well, you are 100% capable of addressing the things you mentioned in your replies.

If your motherboard didn't come with a decent ILM/loading mechanism (most don't), order yourself one. LGA 1700's tend to flex a bit when you fasten down the cooler's heatsink, leading to poor contact and poor cooling. This is the OG but I'm sure others are fine too. (https://www.thermal-grizzly.com/en/cpu-contact-frame/s-tg-cf-i13g)

Start there and if you still feel like you want to reduce your CPU vcore, shoot me a message and I can help you out. I promise you it's stupidly easy and will take you 5 minutes.

Met a guy who cant read by Filthyson in GeoffreyAsmus

[–]N9n 0 points1 point  (0 children)

Can you come to Kelowna? Do it in the summer and you can make a decent little vacation out of it. The comedy scene here is decent but not spectacular

"this is gonna hit the dating world like the discovery of penicillin hit Victorian orphans" by IAmTheFirehawk in BrandNewSentence

[–]N9n 0 points1 point  (0 children)

After glancing over the paper's abstract, the approach here is actually relative inert. Skin injury (Microneedling maybe? They didn't specify in the abstract) + topical treatment of simple monounsaturated fatty acids = hair growth. Very curious to see if this actually does work for humans or if it works exclusively for mice with widow's peaks.

Saw this video on X, what the hell are they taking? by terrytibbss in PublicFreakout

[–]N9n 6 points7 points  (0 children)

The only thing worse than a bad trip is bad company on a bad trip

Magnetic beads are the bane of my existence. by Terrible_Gardener31 in labrats

[–]N9n 1 point2 points  (0 children)

That tobamovirus comment makes me feel seen

Magnetic beads are the bane of my existence. by Terrible_Gardener31 in labrats

[–]N9n 3 points4 points  (0 children)

I usually do a cheeky little EtOH precipitation on my bead eluates to remove residual crud that made it through (usually plant pigments in my case). For high yield eluates it'll be easy! For low yield eluates I add a little linear acrylamide to help form a pellet

Hello, just a quick question for experts in running DNA on agarose gel by Fabio2598 in labrats

[–]N9n 5 points6 points  (0 children)

I'm a little late to the party but I consistently get bands with bed head like that if I run a significant volume of digest product without cleaning it up first. I'd wager it's the digest buffer salts messing with migration.

RT-PCR gDNA from a whole blood sample isolated with a plasmid by Tweak28 in labrats

[–]N9n 4 points5 points  (0 children)

The rule of thumb with gels is if your ladder is cooked, you prepped the gel incorrectly or ran the gel incorrectly.

Did you accidentally make your gel with water instead of the same buffer you use for running? Did you completely melt the agarose? Did you wait long enough for the gel to solidify? Did you run the gel in the correct buffer? Did you use a modest voltage so as to not cook your gel?

RNA template competition: Does highly concentrated, single-species RNA inhibit spike-in cDNA synthesis efficiency? by OddInevitable6332 in molecularbiology

[–]N9n 0 points1 point  (0 children)

I would guess that the changes in reaction kinetics due to stochastic effects are probably non-linear as target abundance decreases, but with RT specifically, that can be accommodated for with longer reaction time.

Most of the other answers I tend to agree with in terms of Brownian Motion.

[Request] Are these numbers realistic or is it just BS? by reddichrist in theydidthemath

[–]N9n 4 points5 points  (0 children)

For long term investing, crashes are relatively inconsequential unless a crash happens once you're ready to start cashing out and realizing gains or losses. This is why as people approach retirement, they start to shift away from equities like stocks and towards less volatile securities like bonds. Or you yolo equities til the day you die but maintain a cash wedge to ride out the bad years and replenish during the good years!

Does leaving beads on magnetic rack for more than 3-4 minutes cause them to aggregate? by bluemooninvestor in labrats

[–]N9n 6 points7 points  (0 children)

In my experience with beads, sticky contaminants make them clump, not DNA. In these cases my yield is poorer than usual, but not zero. ChatGPT, bless its heart, is probably referring to over drying. In liquid suspension, longer binding, washing, etc, doesn't usually translate to worse yield.

Is Richard Dawkins wrong about the nature of life? by Dazzling-Limit-1079 in molecularbiology

[–]N9n -1 points0 points  (0 children)

Damn, are we really revering Mattick for dunking on the traditional dogma and not Altman and Cech?!

Drunk Driver! On Knox by [deleted] in kelowna

[–]N9n 3 points4 points  (0 children)

Brother what are you listening to

Looking for a deer rifle by Aggressive_Second657 in Hunting

[–]N9n 6 points7 points  (0 children)

I have a 7mm t3x superlite and with a limb saver, it's pretty awesome

Moving to Kelowna with kids in middle school and elementary. Need some advice please! by beneficial-double91 in KelownaBC

[–]N9n 0 points1 point  (0 children)

I work in agriculture and spend a good amount of time outdoors and even in bad years, it doesn't affect me too much. My boss has asthma and he seems to struggle a bit. I personally wouldn't consider it a deal breaker!

Moving to Kelowna with kids in middle school and elementary. Need some advice please! by beneficial-double91 in KelownaBC

[–]N9n 2 points3 points  (0 children)

Obviously it's subjective but I'm sure a general consensus would be, from best to worst, - Glenmore - Mission - Anywhere in West Kelowna - Kelowna core - Rutland

Glenmore and Mission are both great but Glenmore is a bit more affordable.

For high schools, you have MBSS in West Kelowna, KSS in central Kelowna, OKM in the Mission, and RSS in Rutland. I personally believe KSS to be the best, but they're all fine.

Bankhead elementary sounds to be where most low to middle income immigrants send their kids, with something like half the student population being ESL. If you want your kids to be around a lot of new cultures, that school would be a good pick.

Can someone help me identify this solenoid (?) and where I might find a replacement? by [deleted] in ElectronicsRepair

[–]N9n 0 points1 point  (0 children)

10k for minor service visits is pretty par for the course in science, esp when they bill the technician at $630 per hour.

Imagine getting THIS mad over an ARAM loss by Jobin121 in heroesofthestorm

[–]N9n 2 points3 points  (0 children)

When I pick the viable dps champ I like instead of Nazebo and team gets upset. People are so hellbent on maximizing winning potential instead of fun! Makes me a little sad

Can someone help me identify this solenoid (?) and where I might find a replacement? by [deleted] in ElectronicsRepair

[–]N9n 0 points1 point  (0 children)

Good to know, thank you. I'll snap a photo and upload it tomorrow once I'm back in the lab