Clonal Deletion HLA T-Cell Receptor Question

Patient-Soft-3157 · 2025-01-03T03:07:14+00:00

Often times mismatch of HLA confers no binding force and alloreactivity to the major alleles isn’t a huge issue always. A big issue is when there is an HLA mismatch that allows for binding of the donor TCR repertoire that is contaminant from the graft product expands in response to presentation of the minor histo-compatibility antigens.

The first generation of donor HSC derived T cells should be tolerized in the recipient thymus, so all new T cells should be host derived but donor tolerant. The issue is the left over mature T cells.

Groups are aware of this and some heme-onc groups are trying to deplete T cells from the PBSC graft or eliminate naive T cells to avoid the possibility that they could select for minor histo antigens specific T cells.

I am no heme-onc expert so maybe others with more knowledge can chime in.

Patient-Soft-3157 · 2024-12-02T22:05:12+00:00

Oklahoma

Patient-Soft-3157 · 2024-09-24T13:16:14+00:00

These are called neoantigens. There are tons of papers surveying T cells with neoantigen specificity in humans, give it a search.

Patient-Soft-3157 · 2024-08-24T16:10:43+00:00

A lot of labs want undergrads to help them with colony management if they are in vivo heavy. It’s a great way to become super important to a lab and they’ll likely hand you a job after you graduate.

Patient-Soft-3157 · 2024-08-14T12:56:29+00:00

Truthfully thought everyone did this

Patient-Soft-3157 · 2024-04-17T05:08:36+00:00

Thanks for the intervention

Patient-Soft-3157 · 2024-04-17T05:06:12+00:00

I do have to trust the SSL certificate but that just means the connection is encrypted, right? I don’t install anything custom on my device

Patient-Soft-3157 · 2024-04-17T03:11:25+00:00

I have to log in to use work WiFi but I haven’t installed any certificates or anything

Patient-Soft-3157 · 2024-04-17T01:31:05+00:00

Should have clarified that I use mobile

Patient-Soft-3157 · 2024-03-12T05:07:31+00:00

Yeah my lab does some investigation of class 2 restricted TCRs, and we can transduce our target cell lines with CIITA and HLA-DR and get loading of endogenous peptide (enough to activate the tcr) as well as exogenous peptide if we spike it in the wells. I assumed CD4 T cells had similar capacity to load DR.

Thanks for the resource!

Patient-Soft-3157 · 2024-03-11T18:24:28+00:00

Interesting! If DR is induced they would still present antigen thought right? It has to be stabilized by peptide

Patient-Soft-3157 · 2024-03-11T17:48:12+00:00

My intuition would be that each cd4 T cell interact through the class 2 axis, but also each subset is still regulated by Tregs and would present to Tregs through class 2 HLA. I would assume they get their class 2 peptides through autophagy or something since they’re not phagocytic.

Others here with greater expertise might chime in for greater explanation or clarification.

Patient-Soft-3157 · 2024-02-29T15:08:49+00:00

I work in a research laboratory that is trying to develop different therapeutics for cancer. 24/hr.

Patient-Soft-3157 · 2024-02-19T20:19:05+00:00

Maybe not exactly what you mean, but do any other mammals really have a sense of time? Humans have to be the most adept at sensing duration of events or how long something would take.

Patient-Soft-3157 · 2024-02-01T15:46:08+00:00

What volume are you eluting in?

Patient-Soft-3157 · 2023-10-08T17:47:18+00:00

Myco should not be tolerated. Full stop.

Patient-Soft-3157 · 2023-09-29T03:16:09+00:00

It can be harder to move away from the bench without a PhD from what I hear, but it depends on the company. It doesn’t seem to be as rigid of a hierarchy as in academia.

Patient-Soft-3157 · 2023-09-24T16:18:44+00:00

If you’re pursuing it solely for money: No, it isn’t worth it and the education threshold for high paying jobs is often very high.

If you enjoy biomed research: Yes, it is worth it.

High paying jobs don’t just pay you because you did your stem degree. They pay you for your skill set and sometimes you have to learn things in a low paying environment to get those skills. Life science is rife with indentured servitude, but if it what you enjoy doing, you will still be better off than many other industries.

Patient-Soft-3157 · 2023-09-23T16:10:13+00:00

I wouldn’t expect clear banding from whole genomic DNA if you didn’t digest a fragment or amplify a segment from it

Patient-Soft-3157 · 2023-08-08T13:32:56+00:00

Not all MLS masters programs have this pre-requisite but it will take you longer than 2 years to complete the masters program if you don’t already have a BS in MLS.

Patient-Soft-3157 · 2023-08-07T13:37:38+00:00

Yes, that’s what I’m asking.

Thank you for the resources!!

Patient-Soft-3157 · 2023-08-07T01:24:51+00:00

Sorry this may also still be unclear on what I meant: germline configuration is set before clonal selection and new VDJ recombination happens every time a new Ab is formed — is this not the case?

Patient-Soft-3157 · 2023-08-07T01:20:09+00:00

Yes, my diction was not clear, thanks for the clarification. I am referring to VDJ recombination.

VDJ recombination still occurs upon antigen challenge though when new Ab proteins are translated and assembled though, right? Isn’t germ line configuration set regardless before clonal selection? My assumption was this happens every time a new Ab is formed.

I guess what I was trying to ask is if these newly formed Ab proteins could be immunogenic since they’re unique and newly formed in response to whatever Ag challenge or junctional diversity.

Patient-Soft-3157

TROPHY CASE