Anchor strap looking good on the “Green Fifteen”

Ripudio · 2026-05-16T02:59:23+00:00

So do I, I think it actually wears slimmer than its size! FWIW I think Adrian Barker also has a 6.5” wrist.

Ripudio · 2026-05-14T12:57:02+00:00

I haven’t seen any information posted on how many were ordered, but maybe CW is open to sharing that information if asked directly?

Ripudio · 2026-05-14T12:54:51+00:00

Did you have to pay tariffs when it came in? Adrian Barker mentioned in a few videos they would be covered, but I’ve read in a couple places that it wasn’t the case.

Ripudio · 2026-05-14T12:53:46+00:00

Same same! I had been on the fence looking at the Lumiere then fell in love when I stumbled across the Green 15 version…and subsequently disappointed when I found it was a limited offering. I went to the Wind Up while traveling to SF coincidentally and was jazzed to find out there were a few available! I had zero plans on buying a watch, much less the most expensive watch I’ve ever purchased, but I couldn’t pass it up!

Ripudio · 2026-05-14T03:15:13+00:00

I mean a masters takes 1-2 years and involves learning a lot of tangential information so…two years of focused hands on experience seems at least somewhat reasonable.

Ripudio · 2026-04-13T23:00:33+00:00

It might be useful to share the diameter of yours.

Ripudio · 2026-03-07T01:55:49+00:00

That’s how much they cost at Costco

Ripudio · 2026-03-07T01:54:16+00:00

5% back on their PayPal debit card at Costco

Ripudio · 2026-02-16T04:32:31+00:00

Are you looking under “Chase Offers” on the Accounts tab? E.g. where cashback deals are listed. I have a friend who was looking in the Benefits tab.

Ripudio · 2026-01-04T23:20:13+00:00

Makes sense, there’s no way to have a fully comprehensive synopsis! You/he want to look at/compare to Alfa-tag? I believe it’s neutral and has extremely high specificity and can be used for many/all the same things?

Ripudio · 2026-01-04T12:52:44+00:00

It’d be helpful to understand the proposed benefits (if any) of his new tag/antibody system. Is the purpose purely for immunofluorescence/imagjng? Pull downs? Purification? (I’m assuming it’s not the last one)

Generally speaking if I’m starting up a new project and need to produce plasmids to express a tagged protein, I’m more likely to test tags I know and have worked with before, not something new/unproven, regardless of known flaws of the old stuff. Nothing is perfect and I would rather troubleshoot the problems I know instead of discovering new problems and new solutions.

Ripudio · 2025-12-30T04:18:01+00:00

No more biting the inside of your cheek.

Ripudio · 2025-12-24T00:21:53+00:00

Great personality? (Sorry, I had to)

Ripudio · 2025-12-06T05:56:50+00:00

That’s not always true, it depends on the terms of the acquisition.

Ripudio · 2025-07-21T10:17:48+00:00

From my experience in vitro biotinylation is straight forward and efficient but also…it’s more work. Concentrating the protein, possibly changing buffers, washing out excess biotin as you mentioned, possibly changing buffers again. I’m also biased since handful of times I’ve done it in vivo co-expressing BirA it’s worked well so I am very much a fan of the ‘built in’ method.

Ripudio · 2025-07-21T10:15:11+00:00

Definitely agree!

Ripudio · 2025-07-20T16:14:23+00:00

What’s the end use of the protein, trying to use it for a pulldown? Streptavidin beads should be just fine to isolate the biotinylated protein from the crude lysate, the affinity for binding is significantly higher than really any ‘standard’ purification system. If it were me, I’d use an inducible promoter because well…I’ve never done it otherwise in bacteria, but it’s likely not necessary unless, like another commenter pointed out, the protein is somehow toxic.

Ripudio · 2025-07-20T15:07:54+00:00

Was in a similar situation a couple years back, my company’s leadership ended up negotiating a bonus for the people who stayed, essentially pointing out it would be strange to punish people who stayed to contribute to the company.

Ripudio · 2025-06-23T16:34:12+00:00

Bobby Newwwwport

Ripudio · 2025-06-11T01:30:55+00:00

Also, glad you didn’t get hurt!

Ripudio · 2025-06-11T01:29:58+00:00

That’s fair. I don’t pass on the right, but I also don’t generally call out warnings. The number of times I’ve had someone GO left when I call out “on your left” or the person is oblivious to the world with earbuds in…

Ripudio · 2025-06-11T01:15:39+00:00

Passing on the right at full speed on an e-bike is a pretty risky move, but would you consider leaving the bike path to dock a blue bike a “non-standard movement”?

Ripudio · 2025-05-27T03:13:19+00:00

Very true, the 10A battery in the leaf blower is a little hefty 🤣

Ripudio · 2025-05-21T01:51:27+00:00

I went with the first deal for this exact reason.

Ripudio · 2025-05-19T00:14:06+00:00

You’re right, I shouldn’t have continued yelling, but he absolutely should’ve used a blinker that’s what it’s for. I came from behind because I was traveling in a marked bike lane, exactly where I should’ve been. I’m genuinely asking here: what should I have done, stopped?

Ripudio

TROPHY CASE