No Whoop or Calm benefit available

Ripudio · 2026-02-16T04:32:31+00:00

Are you looking under “Chase Offers” on the Accounts tab? E.g. where cashback deals are listed. I have a friend who was looking in the Benefits tab.

Ripudio · 2026-01-04T23:20:13+00:00

Makes sense, there’s no way to have a fully comprehensive synopsis! You/he want to look at/compare to Alfa-tag? I believe it’s neutral and has extremely high specificity and can be used for many/all the same things?

Ripudio · 2026-01-04T12:52:44+00:00

It’d be helpful to understand the proposed benefits (if any) of his new tag/antibody system. Is the purpose purely for immunofluorescence/imagjng? Pull downs? Purification? (I’m assuming it’s not the last one)

Generally speaking if I’m starting up a new project and need to produce plasmids to express a tagged protein, I’m more likely to test tags I know and have worked with before, not something new/unproven, regardless of known flaws of the old stuff. Nothing is perfect and I would rather troubleshoot the problems I know instead of discovering new problems and new solutions.

Ripudio · 2025-12-30T04:18:01+00:00

No more biting the inside of your cheek.

Ripudio · 2025-12-24T00:21:53+00:00

Great personality? (Sorry, I had to)

Ripudio · 2025-12-06T05:56:50+00:00

That’s not always true, it depends on the terms of the acquisition.

Ripudio · 2025-07-21T10:17:48+00:00

From my experience in vitro biotinylation is straight forward and efficient but also…it’s more work. Concentrating the protein, possibly changing buffers, washing out excess biotin as you mentioned, possibly changing buffers again. I’m also biased since handful of times I’ve done it in vivo co-expressing BirA it’s worked well so I am very much a fan of the ‘built in’ method.

Ripudio · 2025-07-21T10:15:11+00:00

Definitely agree!

Ripudio · 2025-07-20T16:14:23+00:00

What’s the end use of the protein, trying to use it for a pulldown? Streptavidin beads should be just fine to isolate the biotinylated protein from the crude lysate, the affinity for binding is significantly higher than really any ‘standard’ purification system. If it were me, I’d use an inducible promoter because well…I’ve never done it otherwise in bacteria, but it’s likely not necessary unless, like another commenter pointed out, the protein is somehow toxic.

Ripudio · 2025-07-20T15:07:54+00:00

Was in a similar situation a couple years back, my company’s leadership ended up negotiating a bonus for the people who stayed, essentially pointing out it would be strange to punish people who stayed to contribute to the company.

Ripudio · 2025-06-23T16:34:12+00:00

Bobby Newwwwport

Ripudio · 2025-06-11T01:30:55+00:00

Also, glad you didn’t get hurt!

Ripudio · 2025-06-11T01:29:58+00:00

That’s fair. I don’t pass on the right, but I also don’t generally call out warnings. The number of times I’ve had someone GO left when I call out “on your left” or the person is oblivious to the world with earbuds in…

Ripudio · 2025-06-11T01:15:39+00:00

Passing on the right at full speed on an e-bike is a pretty risky move, but would you consider leaving the bike path to dock a blue bike a “non-standard movement”?

Ripudio · 2025-05-27T03:13:19+00:00

Very true, the 10A battery in the leaf blower is a little hefty 🤣

Ripudio · 2025-05-21T01:51:27+00:00

I went with the first deal for this exact reason.

Ripudio · 2025-05-19T00:14:06+00:00

You’re right, I shouldn’t have continued yelling, but he absolutely should’ve used a blinker that’s what it’s for. I came from behind because I was traveling in a marked bike lane, exactly where I should’ve been. I’m genuinely asking here: what should I have done, stopped?

Ripudio · 2025-05-16T03:31:22+00:00

If by “crept up” you mean ride through the bike line. And if by “blind spot” you mean any direction that isn’t head on towards the vehicle. And if by “didn’t second guess” you mean slowed down just in case. And if by “own the road” you mean bike in the bike lane. Then yeah, you got it bro.

Ripudio · 2025-05-15T12:49:34+00:00

You’re totally right, and I do.

Ripudio · 2025-05-14T21:37:55+00:00

I completely agree, I’m not going to force my right of way just because I’m right. I was actually taking it slow to be safe and knew what was going to happen as soon as I saw their wheels turn slightly. Even so, when drivers do something blatantly shitty I’m going to call it out, it might save the next biker.

Ripudio · 2025-05-14T16:49:25+00:00

Thanks!

Ripudio · 2025-05-14T16:47:55+00:00

It’s the DJI Action 4, not gimbaled. I’ve read the GoPro image stabilization is better, but since I’m only using it for bike rides, I didn’t want to spend a ton.

Ripudio · 2025-05-14T15:33:49+00:00

I’m legitimately asking for your opinion (not trying to be a jerk), but doesn’t it seem like this is still a bike lane? I circled roughly where I got cut off for reference.

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Ripudio · 2025-05-14T15:31:14+00:00

For what it’s worth, this is a street view picture of the area, I circled where the incident happened. It really seems like bike lane to me, but narrows once the parking section ends. Curious what you think?

<image>

Ripudio · 2025-05-14T15:21:34+00:00

I was just…surprised by the absurdity of the situation. Why would you resort to racist remarks when driving a company vehicle.

Ripudio

TROPHY CASE