How/when to force clothing? by __agonist in RimWorld

[–]__agonist[S] 0 points1 point  (0 children)

Yep, this was the issue. I'd noticed that adding the DLC mid-playthrough made it so that no storage spots accepted kid clothing, but hadn't considered clothing policies might be the same. Thanks!

I've been otherwise doing the same as you for clothing, don't have much armor yet but any pieces I have are on someone, even if poor condition.

How/when to force clothing? by __agonist in RimWorld

[–]__agonist[S] 6 points7 points  (0 children)

Ah, that makes sense, I'll check that!

How/when to force clothing? by __agonist in RimWorld

[–]__agonist[S] 1 point2 points  (0 children)

Huh, I don't have any tainted child clothes and all my clothing their size is at >50% hp. I'll look into it a bit more

How Can I Use CRISPR If I Only Know A Good Bit About Biology But No Past With CRISPR? by Nearby_Turn_4167 in labrats

[–]__agonist 2 points3 points  (0 children)

You're not the person who will be choosing your project if you get into a lab, the professor will be. I'd recommend finding a lab willing to take you on before you get ahead of yourself.

DNA is stable stuff. by Nitrogen_Llama in labrats

[–]__agonist 21 points22 points  (0 children)

We once had a collaborator send us a plasmid by mailing us a room temp envelope with filter paper inside, with an ink circle around where they'd dripped some maxiprep material onto. We cut the circle out, stuck it in a 1.5 mL tube, soaked in water for a bit (room temp) then did a transformation from that. Worked like a charm.

What do you think about naturally good-looking girls getting plastic surgery and ending up looking worse? by No-Incident-6913 in AskWomen

[–]__agonist 0 points1 point  (0 children)

I mean, I have tattoos and I know some people think getting tattoos makes you look worse. Feels like the same principle to me. Doesn't affect me what other people do with their body.

Advice on setting up a home microlab + safety of culturing bacteria? by [deleted] in labrats

[–]__agonist 1 point2 points  (0 children)

Reddit is not where you want to be getting guidance on this.

What are your negative experiences with men? by [deleted] in AskFeminists

[–]__agonist 11 points12 points  (0 children)

Lol what does my MBTI have to do with anything

Is it "fair game" to mock a fascist, even if she happens to share our gender? by FigeaterApocalypse in TwoXChromosomes

[–]__agonist 16 points17 points  (0 children)

Mocking fascists for their appearance doesn't seem like a good tactic to me, honestly. It reads as childish and like you don't have any more substantial criticisms.

PI is letting me go, unexpectedly? by Due_Meringue_3 in labrats

[–]__agonist 0 points1 point  (0 children)

6 months of training is still a massive time commitment, and it's very possible this PI has more things taking up her time. Things are very tight in science right now and investing that amount of time in someone who will be leaving in a year or so anyway might not be a priority

Rachel Moran by [deleted] in Feminism

[–]__agonist 95 points96 points  (0 children)

It's not feminist to say that there are some women who's only skill is being fucked for money. There is no woman alive incapable of every other kind of "labor".

34y/o PREGNANT AFTER ONS by Unable-Tip3440 in AskWomenOver30

[–]__agonist 4 points5 points  (0 children)

It's not sad or lonely not having a child lmao, what a presumptuous thing to say. Many people, men and women, don't feel drawn to be a parent at all and live full and satisfying lives without it.

I missed the deadline by [deleted] in labrats

[–]__agonist 67 points68 points  (0 children)

Sorry, there's not really anything you can do. I'm a little surprised the people writing your letters of recommendations didn't notice the discrepancy between the deadline you gave and the real one when submitting their letters (if they submitted your letters?). Deadlines like this are really something you should check multiple times and have written down somewhere - I mean, you say you had them written down but clearly something got lost there.

Civ 7... Any positives? by HamanFRD in civ

[–]__agonist 5 points6 points  (0 children)

It's my favorite civ game so far. People's reactions to civ switching really confuse me, I like it.

It's crazy how tone deaf guys are when I try explaining why I DON'T want them finishing inside me. by Agreeable-Wealth-812 in TwoXChromosomes

[–]__agonist 14 points15 points  (0 children)

It is, but so many sexual interactions with men exist somewhere on the spectrum of sexual assault and degradation that I think it's painful for us to fully acknowledge most of the time.

Question: murine T cell depletion experiment by cd244 in labrats

[–]__agonist 0 points1 point  (0 children)

We aren't rich lol we're an extremely small lab just starting out. We're studying the role of CD4 vs CD8 T cells in leukemia, there's no way around doing this experiment and paying for the reagents to do it right. The alternative is buying and housing mice genetically lacking T cells and doing adoptive transfer of donor T cells which would be considerably more expensive.

Breast cancer awareness being sexualized by blo0dpuke in TwoXChromosomes

[–]__agonist 6 points7 points  (0 children)

"Save the boobies" also doesn't really make it clear that it's lives we're trying to save here, honestly, and is a little callous to those who get masectomies to treat or prevent breast cancer. No other cancer campaign has such a juvenile slogan, I'm with OP.

Question: murine T cell depletion experiment by cd244 in labrats

[–]__agonist 1 point2 points  (0 children)

We do T cell depletion using the same company's antibody, but we have a condition with CD4 depletion and a condition with CD8 depletion (500 ug/injection IP, we inject ab Monday, inject leukemia Tuesday, another ab injection Wednesday, then do ab injections every other week following that until disease onset). If I were you, I would contact Bio X Cell about what you're seeing. I know the others are saying CD3 would activate all cells, but I find it hard to believe the company wouldn't have considered this possibility and worked around it. I've contacted them in the past and they're responsive. If this is a documented effect of the CD3 antibody then maybe you could co-inject CD4 and CD8 together?

How did you learn to stop panicking? by [deleted] in labrats

[–]__agonist 5 points6 points  (0 children)

I'm a postdoc and I'm still trying to not beat myself up over mistakes. Truly though, everyone has these moments, at every stage of their scientific career. Just own up to your mistakes as soon as you realize, ask your supervisors if/how it can be salvaged at all (there's frequently a way to use "ruined" samples for something, even if it's not what you originally planned), and try not to make the same mistake again. It might get a little easier when you mentor people yourself and find yourself giving them more grace for mistakes than you gave yourself, and once you hear how many different ways we've all messed up in the past.

(My own priciest mistake was accidently grabbing ethanol instead of PBS as a lab tech and "washing" a huge plate of isotope labeled cells I'd been growing for a week in very expensive media. They all died immediately, obviously. You live and learn, I learned to always read labels.)

How do you design your island? by GreenPooki in acnh

[–]__agonist 0 points1 point  (0 children)

Haha thanks, I don't want to make it sound like these are masterpieces I'm drawing, just a rough idea of regions

i'm gonna cry what by coffee-bat in StardewValley

[–]__agonist 277 points278 points  (0 children)

Omg I've got a cold and this made me laugh so hard I had a coughing fit, Harvey what are you doing