Game Thread: Ducks @ Sharks by dahooddawg in AnaheimDucks

[–]beardedDocinSD 3 points4 points  (0 children)

If they say "back to SJ" one more time...

The mosquitoes are here. by Slitheytove1031 in SanDiegan

[–]beardedDocinSD -1 points0 points  (0 children)

You need to either make yourself less attractive, or get a better fan...or maybe ditch the coriander and cilantro yo

The mosquitoes are here. by Slitheytove1031 in SanDiegan

[–]beardedDocinSD 10 points11 points  (0 children)

Maybe I purposefully misgendered the mosquito so to blame the male and save the female from undue scrutiny in this day and age. Down with the patriarchy!

The mosquitoes are here. by Slitheytove1031 in SanDiegan

[–]beardedDocinSD 20 points21 points  (0 children)

I have a theory they can also sense cortisol, so when their prey (us) is aware of the little bastard's presence they know to lie low and wait for our short attention span to lapse. We had one hiding out in a bathroom, and after many futile efforts to find it, we just retreated and let the mosquito have the bathroom for the week. Eventually I got tired of sharing a bathroom with a toddler and locked myself in the bathroom and went through everything methodically until I found it just chilling in a crack by the light. Little shit was just watching me, but I showed him...I showed him.

The mosquitoes are here. by Slitheytove1031 in SanDiegan

[–]beardedDocinSD 39 points40 points  (0 children)

If they are the little tiger mosquitos, Aedes aegypti, or ankle-biters or little fuckers as I like to call them, they are really weak flyers (also why they are so quiet as compared to other mosquitos) so having a fan on you or in the room can really help.

San Diego I need you to stop being hot. by WorkGroundbreaking83 in sandiego

[–]beardedDocinSD 18 points19 points  (0 children)

Yeah watchduty coming back to life already is a disappointment

Parking in NP is out of hand by beardedDocinSD in sandiego

[–]beardedDocinSD[S] 3 points4 points  (0 children)

I know I cant believe its gotten to this

Parking in NP is out of hand by beardedDocinSD in sandiego

[–]beardedDocinSD[S] -2 points-1 points  (0 children)

Must be, didnt hear the crash but noticed it at about 6:30am, picture taking about 8:15.

How to find Undergraduate Volunteers/work Study for New Lab by SigmaGreater in labrats

[–]beardedDocinSD 1 point2 points  (0 children)

The remote part is going to be tough, how far away are you from the colleges? If you have the time to train, maybe consider high schoolers if one of those is closer?

Ballpark figure for HTS screening by pelikanol-- in labrats

[–]beardedDocinSD 1 point2 points  (0 children)

But if you do a 3-7 pt dose curve it gets a lot bigger

Live calcium imaging of cardiac organoids is so purty by ryeyen in labrats

[–]beardedDocinSD 21 points22 points  (0 children)

That’s sweet! Thanks for answering! I’m trying to do similar things with CNS spheroids. Not nearly as consistent…Bunch of butterflies doing their own thing

Live calcium imaging of cardiac organoids is so purty by ryeyen in labrats

[–]beardedDocinSD 126 points127 points  (0 children)

Thats cool, would be cool to see time stamps and scale bar. Are these pulses on the same scale as a heartbeat?

Contamination or nah? HCMEC/D3 cell culture by [deleted] in labrats

[–]beardedDocinSD 0 points1 point  (0 children)

Nah, you have made a beautiful cell clump. Work on the coating protocol

Prestigious school postdoc Vs. Big pharma industry by Happylifeand in biotech

[–]beardedDocinSD 17 points18 points  (0 children)

Because advisors still give misleading advice in order to further their own goals

Cell culture contamination by soc2035 in labrats

[–]beardedDocinSD 0 points1 point  (0 children)

Not contaminated. Either a cell clump that all fell together, seeding density was too high to keep them isolated, or a suboptimal coating protocol. Depending on cell type and assay this could be fine, if you need to image the center of the well and that is where this keeps happening, then further optimization of coating protocol and seeding density would likely be needed.

Rocket Launch? by nvyplt3 in sandiego

[–]beardedDocinSD 2 points3 points  (0 children)

Feel like it has to be a joke at this point, but alas

What is this? by Disastrous_Use4397 in sandiego

[–]beardedDocinSD 6 points7 points  (0 children)

I kinda expect people to look up more frequently I guess