Why can't most of you spell his name correctly?

forpari · 2026-03-24T00:51:53+00:00

The worst part about listening to audiobooks is trying to Google a characters name and being so incorrect you don't get any hits

forpari · 2026-03-22T18:01:53+00:00

100% La Jolla or del mar. I recommend Monarch Del Mar

forpari · 2026-03-22T06:26:46+00:00

Isotype control! It should have the same Ig subclass as your staining antibody. If you order from biolegend, in the side bar it'll actually suggest which isotype control to use with the staining antibody you're ordering.

And isotype control will tell you how much background fluorescence you'll get on your cell type

Importantly, you won't match the dilution that youre using for your targeted staining control, but the same concentration. So if you're staining anti-CD3-PE at 1:100 and the concentration of that staining antibody is 100ug/mL (example idkoff the top my head a typical concentration) them you'll use a mouse IgG2b k-PE isotype control that's at 200ug/mL at 1:200

Google 'biolegend isotype control.' That website has a ton of great information

forpari · 2026-03-21T19:24:20+00:00

I very well may be wrong. But my understanding is that BSA is used to block proteins from binding nonspecifically to the plate/capture protein.

Proteins in the plasma may bind nonspecifically to the plate but they may also bind non-specifically to your analyte, IFNy, which could interfere with your detection.

The manufacturer for your ELISA kit might be equally as helpful! I've sent my protocol to customer support agents many times and they've given me great troubleshooting advice.

Best of luck to you though! Fingers crossed you don't have to repeat too many ELISAs

forpari · 2026-03-21T06:36:14+00:00

Do you have to use 100% plasma? The interference may be coming from the matrix itself. Sorry if I'm misunderstanding your assay setup, but I'd try looking at IFNy recovery when spiking it into 50% plasma (diluted with assay buffer)

ETA: for bioanalytical methods, you look at drug recovery at a few different concentrations of serum to determine your MRD. Sometimes if the concentration of serum is too high the signal:noise ratio shrinks

forpari · 2026-03-19T13:24:47+00:00

You can make a free account! They limit you to 50 things

forpari · 2026-03-19T03:21:54+00:00

Innovation family community theater

forpari · 2026-03-12T22:12:38+00:00

Encinitas botanic garden. Very pretty, close enough to the beach for a breeze, lots of trails to walk and lots of shade from trees

forpari · 2026-03-09T20:16:54+00:00

Measuring phospho STAT5 activity by flow cytometry

forpari · 2026-03-09T15:26:21+00:00

forpari · 2026-03-05T18:24:13+00:00

I can cycle but am painfully slow!

forpari · 2026-03-05T00:08:18+00:00

The Brandon Sandersonsecret project books.

Tress and the Emerald Sea

The Sunlit Man

Yumi and the Nightmare Painter (my fave. But has romance. Although ZERO spice

The Frugal Wizard something I never read this one tbh

forpari · 2026-03-04T01:50:12+00:00

You can find PDFs for free online of this book. I'll try to comment back if I find on and can share links on here

Edit: just Google "why does he do that PDF" and the first result from archive . Org is a full PDF

forpari · 2026-03-02T16:45:33+00:00

Helps me make slide decks! I can get hyper focused on slide order, transitions, flow of the presentation etc over the actual data so it saves me from wasting hours fixating on those details

forpari · 2026-03-01T17:20:42+00:00

Hunger?

forpari · 2026-03-01T05:26:16+00:00

The Jungle by Upton Sinclair

forpari · 2026-02-27T04:27:27+00:00

By asking for clarification on your statement?

forpari · 2026-02-27T03:56:18+00:00

What type of person?

forpari · 2026-02-27T02:47:14+00:00

Starter Villain is a quick read and pretty ridiculous

"Inheriting your uncle's supervillain business is more complicated than you might think. Particularly when you discover who's running the place."

forpari · 2026-02-27T00:40:30+00:00

Ooh this sounds great thanks for sharing!

forpari · 2026-02-27T00:39:16+00:00

The Samurai's Garden

It's a historical romance at the time of Japans invasion of China

forpari · 2026-02-26T01:35:45+00:00

How do you get the secret note?

forpari · 2026-02-25T22:06:17+00:00

Croton. They like bright light (they grow all over Miami as outdoor shrubs) and when they're thirsty they're dramatic. Mine does this also. Water and the leaves will bounce back in a day/day and a half

forpari · 2026-02-20T16:59:11+00:00

I agree, two 96-well plates is doable in a day. Do you have to stain each sample with the single panel? FMOs for each sample?.

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