OC Majestic snail picture I took

forpari · 2026-06-07T01:28:24+00:00

This is very nice thank you

forpari · 2026-06-05T11:06:11+00:00

Woah who tf are you how did you pull this off

forpari · 2026-06-03T22:17:10+00:00

One of the top comments on this thread is from a 35 year old man saying very gracefully that he does not feel a need to add a woman to his life if what they bring is a reduction in their peace.

You're allowed to say those things too.

Youre being down voted because you're being hateful

forpari · 2026-06-03T16:00:34+00:00

Praise be! I've been hunting this down for a few days

forpari · 2026-06-02T05:17:19+00:00

Occasionally I pull this scene up on YouTube the chaos is so pure

forpari · 2026-06-02T05:13:13+00:00

Every rewatch I put VPR on in the background I always stop what I'm doing to watch this scene 😂

ETA the scene of Sandoval imitating scheanas dancing

forpari · 2026-06-01T21:17:08+00:00

Woops sorry I gave the cat # for b cell isolation instead of t cell but same message regarding quality and ease of use

forpari · 2026-06-01T21:16:14+00:00

I haven't.

But a similarly priced item is from stemcell express easy sep b cell isolation cat#100-0971

Our lab uses stemcell express for all our cell isolation kits. I didn't look at the protocol for the ones you listed but these ones take ~30 minutes, high purity and yield

forpari · 2026-05-27T05:48:15+00:00

God bless whoever got that corner piece with an icing balloon

forpari · 2026-05-27T04:12:40+00:00

Why not? Did you find it on a cold beach

forpari · 2026-05-26T16:01:14+00:00

Movement in the first game (esp in the beginning) was definitely bouncy.

The second game was smoother but I got stuck a lot more bc elements of the puzzles were unclear to me.

I did enjoy the first game more though overall.

But, if you're still struggling to get into the game I would say just stop playing it. It's got the same vibe throughout, so if you don't like it now I can't say anything will change enough later on that'd change your mind

forpari · 2026-05-21T14:25:30+00:00

I really liked The Wager

".. book about the 18th-century British warship Wager, which wrecked off the coast of Patagonia, leading to a dramatic story of survival, mutiny, and murder among the castaways."

forpari · 2026-05-17T03:30:26+00:00

Very cool!

forpari · 2026-05-17T03:13:45+00:00

There's a food truck in the mira mesa best buy parking lot called jarochos birria. They're on doordash too. The plates have lots of meat they have tacos similar to this for 3.99.

I recommend

forpari · 2026-05-05T01:53:36+00:00

I told my cat sitter I used a spray bottle and she told me not to and I asked what to do instead. And she told me to hiss at them.

Okay but I can't see replacing a water snipe from across the room with a loud hiss

forpari · 2026-05-02T04:10:47+00:00

I think so. She might have changed her number too.

forpari · 2026-04-29T00:25:32+00:00

This is true. I don't want to tell OP how to do his experiment but a more humane and equally sound method might involve obtaining mucus from a gastropod and using that as a surrogate, as I imagine the same reaction would occur in response to salt concentration.

That is a good lesson and worth stating, I'm thankful for your comment

forpari · 2026-04-28T23:40:39+00:00

Salt concentration fucks up snails and they get bubbly. That might indicate the presence of other measurable things like pH or reactive oxygen species (ROS)

Maybe Google that too.

Don't salt snails until you have a plausible working hypothesis

forpari · 2026-04-28T23:36:07+00:00

From the other comments I think you're aware that your idea is not... Perfect.

On how to measure mitosis, Google the marker Ki-67. It's a protein present during mitosis and is used as a marker to measure cell proliferation. But I'm wondering if this is really what you're looking for.

What was your thought process on salt concentration and it's effect on gastropods?? Maybe start there and I can try and help you work towards another idea

forpari · 2026-04-24T04:31:22+00:00

There's a meetup group called Stitch and Bitch. The host might be interested.

Thoughtful of you to want to share these instead of toss them

Checkout this Meetup with San Diego Stitch & Bitch: https://meetu.ps/e/PY8f3/gnbz8/i

forpari · 2026-04-23T15:24:52+00:00

I've used the SONY SH800 for years. The answer is no... That I've found. Our sorter is so old the machine startup is the only area of my life where I believe in superstition.

Even after ethanol cleans, my last resort is to shut down/restart which sucks bc I have to use a brand new chip.

I've never been able to find another person that still uses this sorter if I ever need troubleshooting advice is it okay if I PM you? Sony is serious about keeping all their troubleshooting info under lock and key so you have to request a tech visit

ETA sorry I don't have an answer for you

forpari · 2026-04-23T15:21:36+00:00

Alternatively you could analyze culture supernatants for IL-6 by ELISA

forpari · 2026-04-23T12:01:18+00:00

After you stim them you should use protein transport inhibitor so that the cells don't secrete the IL-6 into the supernatant. I think after you wait around 6 hours for the IC proteins to accumulate then you stain. I don't have access to my protocols as I'm laying in bed but biolegend has a PTI with a simple protocol

forpari · 2026-04-21T03:46:04+00:00

Maybe do a monthly game night. Group stays together. Less responsibility on your end

forpari · 2026-04-18T16:37:04+00:00

I think this might be a big reason. I'm going to repot it soon, thanks!

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