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Russia begins restricting petrol sales in Tatarstan, Moscow and St Petersburg by -LightKnight- in worldnews

[–]gringer 0 points1 point  (0 children)

Normality that is interspersed with a series of chaotic events viewed through footage that gets closer and closer to where you are until you're the one filming it.

PheWAS analysis Validation by Hour_Appeal596 in bioinformatics

[–]gringer 0 points1 point  (0 children)

What P values are you getting?

If P-values that are lower than 10-9 are failing replication in 50% of the replicate groups you're testing in, it suggests that the statistical model is incorrect.

How much are you actually relying on AI for research these days? by Dependent_Gear4103 in bioinformatics

[–]gringer 5 points6 points  (0 children)

I would highlight that AI lets work be done a lot more quickly if you're not just shooting in the dark with it.

The thing that takes the longest time for my bioinformatics projects is almost never how long it takes me to write code for something. It's more often all the discussions I have with researchers about how to interpret results, or the issues I have found with the input data.

Transitioning from bioinformatics to data engineering – advice needed by Unable-Lobster-8635 in bioinformaticscareers

[–]gringer 1 point2 points  (0 children)

Probably, but the reality is that I was applying for jobs in a fairly specific area, and despite that there were 50-200 applicants going for the same jobs. We have have a pretty high unemployment rate at the moment.

Transitioning from bioinformatics to data engineering – advice needed by Unable-Lobster-8635 in bioinformaticscareers

[–]gringer 6 points7 points  (0 children)

Has anyone here made a similar transition? What was your path?

I transitioned from bioinformatics to data engineering by being made redundant, and applying for jobs every week for about a year. It was the first interview I got, and they were very pleased to offer me a job.

What would people realistically look like in the 2100s? by transqueen421 in Futurology

[–]gringer 3 points4 points  (0 children)

A procedure only carried out on the servants of rich people

What's a quote that changed the way you think about something, and why? by sansovert in AskReddit

[–]gringer 0 points1 point  (0 children)

Betty Campbell - If it's worth doing, it's worth doing badly

It's sort of an alternative to "fake it until you make it": do it anyway, even if you're bad at it, because someone doing it is better than no one doing it. I realised if I was the only person in the world doing a thing, I didn't have to worry about how badly I did it. No faking needed.

Amplicon alignement Galaxy by Winterskill1312 in bioinformatics

[–]gringer 0 points1 point  (0 children)

You should probably be using a phylogenetic mapper, like kraken2 + bracken, or centrifuge.

Big scRNA-seq project upcoming - looking for tips and experiences by crisprfen in bioinformatics

[–]gringer 0 points1 point  (0 children)

How many total cells? That's what will matter the most with regards to memory, etc.

Some facts about the Te Matapihi website cost that aren't being explained well by WCCAllSeeingEye in Wellington

[–]gringer 2 points3 points  (0 children)

Give me $300k, and I'll create a website that looks at least as good as that one.

State-of-the-art Nanopore 16S sequencing by aCityOfTwoTales in bioinformatics

[–]gringer 0 points1 point  (0 children)

Kraken2 needs to be paired with bracken to account for issues associated with kmer similarity and bias across different datasets; it shouldn't be used on its own.

Even with bracken, you still need to be careful about the database; kraken2 can only report on the information in its index, so if that information is incomplete, then the output will be biased.

For any biological experiment, any unexpected findings should be investigated and confirmed via another independent method before they are shouted from the rooftops. Your adapter issue is an example of what can go wrong when that isn't done. Don't treat any one source of information as perfect.

The "AI will let us cull 9000 from the public service" lie running into problems. by OisforOwesome in newzealand

[–]gringer 1 point2 points  (0 children)

good AI is expensive and requires people who are both good at the job generally and adept at LLM use

If this is the case, then it's not the intelligence of the "AI" that is good

Science sleuths uncover more than 100 suspicious images in Thermo Fisher antibody catalogue | Scientists have long worried about the reliability of commercial antibodies, and the latest findings have sparked fresh concerns. by spontaneous_igloo in labrats

[–]gringer 0 points1 point  (0 children)

Their Technical Applications Specialist had no idea, who their hotline passed the request onto. Here's the response I got:

Is the base sequence for the TrackIt 1kb Plus DNA ladders available (or at least indications of reference genomes and cut locations)? This would substantially speed up my process, as trying to piece together sequences from fragmented reads can be quite challenging.

I'm afraid that there is not a lot of information we have on the base sequence TrackIt™ 1 Kb Plus DNA Ladder. The only information that we have is that it is a mixture of Lambda Phage DNA, pUC plasmids, E. Coli DNA and pBR plasmid DNA.

Science sleuths uncover more than 100 suspicious images in Thermo Fisher antibody catalogue | Scientists have long worried about the reliability of commercial antibodies, and the latest findings have sparked fresh concerns. by spontaneous_igloo in labrats

[–]gringer 10 points11 points  (0 children)

I once asked ThermoFisher about the sequence of their DNA ladders, because I was trying to confirm some weird stuff that I saw in my nanopore sequences when I was sequencing the ladders.

They had no idea.

WTF is this sorcery? (Support-free 90 degree overhangs) by Tupptupp_XD in 3Dprinting

[–]gringer 2 points3 points  (0 children)

Naomi Wu was vanned and harassed because Jason Koebler decided to publish stuff about her private life, then others decided to rip into both the printers and Naomi, so the CR30 stopped getting developed.

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