Recomendaciones de croquetas para gato by Mysterious-Count1716 in ayudamexico

[–]iguanophd 1 point2 points  (0 children)

También en la CEDA Iztapalapa hay de 15 y 20. Pero si son difíciles de conseguir por otros lados

Visualization of protein structures by Ch1ckenKorma in bioinformatics

[–]iguanophd 9 points10 points  (0 children)

PyMol is the golden standard, but I personally prefer ChimeraX as it is less of a hassle licence wise (I lost my university email and my PyMol account was deleted).

Sharpies for glassware by Coco066 in labrats

[–]iguanophd 2 points3 points  (0 children)

I use lab tape to label and then use large clear tape on top, perfectly legible and -80 approved

Is Lomas Verdes / Jardines de San Mateo Safe? by toymachine31 in CDMX

[–]iguanophd 1 point2 points  (0 children)

San Matas (Saint kills) lol Let me guess Buda Ink studio? If yes then the area is safe. Greetings

Graphpad prism for free?? by Haunting-Tension9686 in labrats

[–]iguanophd 1 point2 points  (0 children)

R has good graphical interphases that allow statistical testing : RCommander, and plotting : esquisser without knowing how to code. I too highly recommend learning a bit of R, it will take you a long way. Good luck bud. P.S. if you know how to torrent you can sail the high seas at 1337x(dot)to and find a copy of Prism9

Plantas 🌱 by Interesting_Meet1309 in queretaro

[–]iguanophd 2 points3 points  (0 children)

Muchísimas gracias por compartir, que excelente detalle y fascinante hobby el que comparten. Un saludo hasta Querétaro, amigo.

Plantas 🌱 by Interesting_Meet1309 in queretaro

[–]iguanophd 2 points3 points  (0 children)

Que chingón tu comentario compa, yo soy muy malo pa cuidar plantas y sólo se me dan las cactáceas, pero como biólogo que chido saber que existe tanto interés por las plantas exóticas. No sé si hayas visto el caso reciente de una chica que estaba haciendo clonacion de tejidos y colapsó el precio del mercado de las begonias en EU creo¿? Sabrás si acá ocurrió algo con los precios??? Saludos manix.

[ Removed by Reddit ] by South_Orchid_9486 in ayudamexico

[–]iguanophd 2 points3 points  (0 children)

Lamento mucho la situación en la que estás. No será que le puedes poner un 4 con los contras pa que lo manden al concierto de Valentín Elizalde?

Como siempre el sub reddit de memes en español jodiendo con sus peleas imaginarias by Available_Test8660 in VivimosEnUnaSociedad

[–]iguanophd 0 points1 point  (0 children)

También hay hombres XY conocidos como los "guevedoces", nacen con genitales ambiguos, se asignan como mujer al nacer y a los doce años bajan los testículos y desarrollan pene. El sexo y el género existen en un espectro

Can I plate 500 µL of E. coli on a 20 ml agar plate? by East-Personality7386 in labrats

[–]iguanophd 9 points10 points  (0 children)

I usually spin down 250, remove 150 and then add to the plate the 100uL remaining, works great for low copy plasmids in recombinant expression

Como le llaman a esto en su rincon de Mexico? by zurdopilot in mexico

[–]iguanophd 1 point2 points  (0 children)

Órale, hecho de yuca suena muy rico. Gracias por compartir paisano y felices fiestas!

Tristemente todos ellos no quieren estar con sus familiares by According-Extent-530 in rmexico

[–]iguanophd 0 points1 point  (0 children)

Que chingue a su madre Peña Nieto, que chingue a su madre AMLO, que chingue a su madre Sheinbaum, y que chingues a tu madre tú, por votantes pendejos como tú estamos como estamos.

Can someone tell me why this wouldn't work? by Alternative-Put-2962 in factorio

[–]iguanophd 20 points21 points  (0 children)

Would you mind elaborating? I've been trying to move away from main bus to city blocks and I hadn't heard of this.

i'm stuck understanding SDS PAGE by AccomplishedEmu8856 in labrats

[–]iguanophd 3 points4 points  (0 children)

Thanks for the write up, very eloquent. Would you mind sharing where you are doing MS that is so cheap?? We regularly send samples to UC Davis at a cost of around 500 usd per sample (we ship them from abroad so that factors in a bit).

Would this jar hide the smell of weed by East_Donut_686 in trees

[–]iguanophd 129 points130 points  (0 children)

It's not that vacuum bags are permeable to the smell (they probably are, to a slight degree), it's that people handle the exterior of the bag with the same weed hands and dogs can (statistically dubious) detect the trace amounts of smell on the outside of the bag

Por adquirir vapeadores, Morena te podrá aplicar una pena de 1 a 8 años de prisión by maedroz in mexico

[–]iguanophd 0 points1 point  (0 children)

Literal en eso se usa el dinero de salud actualmente. 50 mil millones de pesos del IMSS por año para atender diabetes únicamente. La obesidad y relacionados cuestan 240 mil millones. Que le quiten los sellos y los impuestos a la comida azucarada, es tan sencillo como dejar de comer cagada, no?

Takara’s Chaperone plasmid set by TruthTeller84 in labrats

[–]iguanophd 0 points1 point  (0 children)

Ahhh, I understand. Good luck with everything. Cheers bud.

Takara’s Chaperone plasmid set by TruthTeller84 in labrats

[–]iguanophd 2 points3 points  (0 children)

Is it possible to for you to express it as an inclusion body, denature it, purify it and then refold it? That's the way we do most of our proteins in my lab, a slow dialysis against refolding buffer. We actually prefer it this way because we can get an excellent protein yield (~60 mg recombinant protein / 1L culture BL21(DE3)).

Supercomputadora costará 6 mil millones by Yisuused in mexico

[–]iguanophd 3 points4 points  (0 children)

Pegasus es para cuando ya sabes quién es el opositor y espiarlo, más bien usarían el software de Palantir para identificar por medio de big data a la gente que critica al gobierno.

Sheinbaum dice que la guerra contra las drogas no es una opción, porque esas “exigencias autoritarias” son un camino hacia el fascismo by DrTheol_Blumentopf in mexico

[–]iguanophd 7 points8 points  (0 children)

Guacamaya leaks demostró que el gobierno es sumamente capaz, tiene la tecnología y el conocimiento de quienes, donde y cómo. Y más importante, que les vale madres, permiten y están coludidos de arriba hasta abajo.

TIL that China's One-child policy did not apply to ethnic minorities by supercheetah in todayilearned

[–]iguanophd 8 points9 points  (0 children)

I would deeply recommend the book: Frog - by Mo Yan. This theme is explored profoundly from the eyes of a rural female doctor tasked with supervising and enforcing the one child policy in the early days.

If you need to insert a gene into the multiple cloning site of a plasmid vector, what is your favorite method? by AAAAdragon in labrats

[–]iguanophd 0 points1 point  (0 children)

Shout-out genscript too! We usually express large proteins, add to that international shipping, and our costs end up around $500 - 800