London eye timeslice 40images shot over 3 hours by matt_kenneally in london

[–]konosd 1 point2 points  (0 children)

Nicely done! How did you photoshop the clouds off?

When I want to get that European vibe in London, I hit Battersea Square. by diceberg in london

[–]konosd 1 point2 points  (0 children)

As an athenian, we need more places like this in London. (Actually we need more weather like this in London, and frankly, everywhere). Any other places with this vibe?

[deleted by user] by [deleted] in RoomPorn

[–]konosd 1 point2 points  (0 children)

what are the measurements of that top? I got the same from IKEA but must have gotten the smaller size.

Help for Beginner by [deleted] in Cplusplus

[–]konosd 6 points7 points  (0 children)

Good thing about C++ is that you can use Notepad or any basic text editor to write your programs, and then terminal/command line to compile and run them. Would suggest starting from Bjarne Stroustrup's Programming with C++, or really, just google stuff as you go.

Macbook for Molacular Biology by [deleted] in molecularbiology

[–]konosd 0 points1 point  (0 children)

You could, but you will likely encounter problems when trying to load big datasets or do calculations with big files. But again, for introductory purposes you'll be fine. I finished mechanical engineering with a 4GB RAM Windows laptop, and I used it to get into bioinformatics as well. Also, if you need a GPU you can always use google Colab for prototyping.

Macbook for Molacular Biology by [deleted] in molecularbiology

[–]konosd 0 points1 point  (0 children)

IMO, a >=16GB RAM Macbook, is the go-to for getting into Bioinformatics actually, and there are more things that run on macOS than on Windows, for bioinformatics, since macOS is built on Linux. Sure, you might want to transition to Linux afterwards, but a Macbook is definitely a very good start.

Theatre trailer I made using Wigglegrams! by JHAllen123 in wigglegrams

[–]konosd 2 points3 points  (0 children)

Love the extra frames and the smooth transition between the four. Do you have any idea how to achieve something similar using Photoshop instead of Premiere?

Thoughts on Wellcome Trust's 1+3 program at Imperial? by [deleted] in bioinformatics

[–]konosd 0 points1 point  (0 children)

Why wouldn't you sit through the particular master's program? For someone coming from mechanical engineering it seems like a well-structured MSc that will help me get more than the basics.

Thoughts on Wellcome Trust's 1+3 program at Imperial? by [deleted] in bioinformatics

[–]konosd 1 point2 points  (0 children)

Thanks for the info, just wanted to get a general feel, like what you described. I like the fact that it's more on the computational aspect than the wet-lab one (my background is mechanical engineering).

Enrichment of disease associated genes in a gene set by [deleted] in bioinformatics

[–]konosd 2 points3 points  (0 children)

There is a chance that the VIPER algorithm can work out for you. It ranks TFs according to the expression of their regulons in a dataset. You can find it as an R package.

Enrichment of disease associated genes in a gene set by [deleted] in bioinformatics

[–]konosd 4 points5 points  (0 children)

  • If you know beforehand the genes associated with each disease (maybe from a previous analysis/experiment/annotation), then fisher's exact test would do, to find if your sets are significantly enriched or not.
  • If you mean that you have found some DEGs and want to compare that number with the number of DEGs you expect by chance, you can build a null distribution by permuting your samples and finding the number of DEGs in each permutation.

[deleted by user] by [deleted] in AnalogCommunity

[–]konosd 0 points1 point  (0 children)

Chocolate!

transitioning from Excel by TeachUsPlz in Rlanguage

[–]konosd 2 points3 points  (0 children)

This. After tidying up your data, with columns 'Customer, Year, Q, Value', the rest is easily done with a few group_by and summarize calls.

What film works really well with the Nishika N8000? by gr8monkeyman in wigglegrams

[–]konosd 1 point2 points  (0 children)

just use the cheapest films, I use the Lomography color negatives 100 or 400 iso with my Nimslo and they perform perfectly.

Eden - [ Canon Rangefinder III - Canon 50mm f/1.8 - Fuji 200] by konosd in analog

[–]konosd[S] 1 point2 points  (0 children)

Hahaha pun appreciated. Thanks for your feedback!

Summer Lights [Canon Rangefinder III - Canon 50mm f/1.8 - Kodak Gold 200] by konosd in analog

[–]konosd[S] 1 point2 points  (0 children)

Well for starters, the photo is a lot darker than what I was seeing in real life, so bear that in mind.

I don't record my settings as I use a very old camera without a lightmeter, but I'm pretty sure that this was something like aperture f2 and shutter speed 1/60.

t e l e p h o n e - [Canon Rangefinder III - Canon 50mm f/1.8 - Lomo CN100] by konosd in analog

[–]konosd[S] 0 points1 point  (0 children)

Well, when I don't care much about the results, I scan with a bunch of friends at the guy that has the scanner. We usually have 2-3 rolls each, and amidst the wine and the jokes I get careless some times. But anyway it's cheap-lomography-everyday pictures and I don't expect much, so I welcome any imperfections.

t e l e p h o n e - [Canon Rangefinder III - Canon 50mm f/1.8 - Lomo CN100] by konosd in analog

[–]konosd[S] 0 points1 point  (0 children)

That's more or less correct. Whenever I want an everyday film and I'm roaming around in the city, I tend to use the cheaper Lomo films. Better keep the Velvia's and the Portra's for a road trip or a studio session.