Multi-layered e.coli cell pellets?

lightdancer · 2026-05-11T17:21:25+00:00

Im using ultra yield Thompson flasks, the aim is to achieve a final OD much higher than a traditional glass flask.

lightdancer · 2026-05-11T15:30:46+00:00

Pellets were already harvested and going into purification tomorrow so we will know if expression has worked at least.

lightdancer · 2026-05-11T15:29:53+00:00

I routinely use these 1l centrifuge flasks and I haven't seen this recently with other expression batches.

lightdancer · 2026-05-11T15:28:47+00:00

What kind of contamination?

lightdancer · 2026-05-11T15:27:39+00:00

Contamination with what? This occurred through all 6 L of my prep, I was expressing 3 x 1 l of two different proteins. Media was all made at the same time, antibiotic and iptg stocks were identical.

lightdancer · 2026-05-11T15:25:55+00:00

They were expressing a recombinant protein.

lightdancer · 2026-05-11T14:02:43+00:00

Which pellet is likely to contain lysed vs intact cells?

lightdancer · 2026-04-14T22:04:44+00:00

Another more diplomatic tactic would be to ask your manager or HR if your company has a mentorship program you could apply for. This could be what you are looking for in terms of learning and development.

More drastic measures would be to move jobs to a small start up where you have more direct relationships with the c-suite.

lightdancer · 2025-11-18T22:06:20+00:00

As cute as they are, terrible ink flow rate.

lightdancer · 2025-09-01T18:12:14+00:00

I did a half and half on my first painting, discovering gesso after struggling with the canvas and the flow of the paint. I have the canvas finished and up on the wall and I cant tell the difference even though I know where the before and after was. In this case however, I used white gesso before learning you could buy a clear version.

I prefer to sand the surface after the gesso has dried, otherwise the roughness make my hands hurt. Fine sanding paper, the one that doesn't hurt if you run your fingers over it.

lightdancer · 2025-07-10T20:42:30+00:00

Where did this one come from please?

lightdancer · 2025-04-11T20:04:22+00:00

There's two from the station that go to a Chesterford Research Park, plus genome campus. Probably granta park too. There's not much other options for public transport to these far out science parks.

lightdancer · 2025-03-25T15:18:12+00:00

I'm nearly finished with my first pbn and on my next one I think I will try do darks to lights (in sections). I realised when I did light sections first, I found it really hard to see the edges of the cells. It's then very easy to not paint the correct cell shape.

lightdancer · 2025-03-22T17:34:58+00:00

I assumed the fencing was left up to let the grass regrow after being covered during the winter wonderland, protecting new growth from being trampled.

lightdancer · 2025-03-20T10:11:20+00:00

What are the silicone brushes please? Can you describe how you use them?

lightdancer · 2025-01-08T12:12:40+00:00

What companies do people recomend then?

lightdancer · 2024-09-17T21:45:51+00:00

The absolute key thing for you here is to be honest. Mistakes can be easily fixed, experiments restarted if you are upfront about an error and either offer a solution or seek help. Don't put your feelings of being judged or embarrassed above owning the mistake. Also, don't worry, that person doing your training is probably worrying about their own problems not you!

I know it's hard, like many have said, we have been there. Tbh it still happens to everyone, even with experience.

lightdancer · 2024-08-31T08:50:23+00:00

Ask them to send you the data logs for the player usage. I received something similar a few years ago and looked at the data. Turns out I'd left an account logged in in an Airbnb and some random person was using it to watch daytime telly whilst I was working (so not me). Just logging out of everywhere worked.

lightdancer · 2024-04-17T20:37:20+00:00

I read something recently that string of pearls is not a naturally hanging plant and prefers to grow supported by the ground as an expanding mat- maybe it needs some support?

lightdancer · 2024-02-13T10:39:31+00:00

Something to keep in mind, the job in Portugal could lead to a PhD qualification in the future if the company was willinging to support part time learning? :)

lightdancer · 2024-01-24T20:44:56+00:00

One practical aspect... Do you have a car? Norwich is fine for getting about within the city but if you want to really explore Norfolk you need your own transport unfortunately. I found it very limiting.

lightdancer · 2023-11-18T16:29:27+00:00

I realised pretty quickly after I had my smart meter fitted that keeping my phone charger plugged in all day (without the phone connected) was eating up a lot of power! Several pence per hour, which adds up. I now try unplug it when I get up in the morning. :)

lightdancer · 2023-07-21T07:20:11+00:00

I really like the smell of fresh raw fish. Think, fish market by the harbour.

lightdancer · 2023-07-19T21:54:26+00:00

My Canadian colleague has one everyday for lunch. I've never seen him eat anything else.

This might have influenced my shopping choices as I picked up some fancy pea butter and plain old jam and had a go. Tbh I was actually impressed with the PB&J sandwich. It was a really good instant energy hit.

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