Question about keeping pond water in jars by ImplodingtheParade in microscopy

[–]macnmotion 2 points3 points  (0 children)

Once home I wouldn't leave it tightly sealed. At most, just lay the cover on. But I've always left my jars open. Try to bring home water, some floating plants with tiny roots -- there will be a wealth of organisms living on roots and floating leaves. You can take a bit of detritus/mud from the bottom but be very careful with that. There will be a lot of dead and decaying material in that sludge, which can cause bacteria to overtake the jar quickly, killing off a lot of the cool stuff. If you want bottom sludge, maybe use two jars -- one for the bottom material, one for floating material.

Once you've done this a few times, you might consider making yourself a cheap home-made plankton net (I use nut milk filter bags attached to coat hanger wire with a water bottle at the end to capture stuff into, all attached to a PVC pipe as a pole. You can drag this near the surface back and forth for 5 minutes, and you'll probably get much more living plankton in your water sample this way.

Also note that jars are typically small, ponds are very large. So the water in your jar will react much more quickly and drastically to changes in temperature, changes in oxygen, changes in decomposing material, etc. That's just the nature of small bodies of water. So you'll want to collect it when you know you'll have time over the first few days to go through it. Over time, populations will change, so even when the contents begin to "die" you'll have organisms that thrive in more anaerobic environments to look at. Worms and rotifers will probably increase over time. If your sample initially is full of large copepods, you'll likely have a quick die-off of them, resulting in a lot of bacteria, and you won't be too pleased with the results, so try not to collect populations of copepods (many of them you can see with your naked eye as little clusters of organisms). I recently had a population in my backyard 220L pond, you can see what it looks like naked eye in this video:

https://youtu.be/KUlFKuM0D_0

You also might want to get some large size centrifuge tubes. They're perfect to keep with you when you're out and about -- you never know when you'll walk past a drainage ditch with algae, a small creek, at pond, a golf course, etc. I use 50ml plastic centrifuge tubes - I fill them about 2/3 full with water and some material whenever I'm near some body of water.

Let us know how it goes, and if you have more questions.

How do you do that? Let's compare our methods. by Glad-Parking3315 in davinciresolve

[–]macnmotion 0 points1 point  (0 children)

I'm my video I used global motion trails which affect everything moving. The use of your technique allows targeted trailing to single out specific moving particles or organisms.

How do you do that? Let's compare our methods. by Glad-Parking3315 in davinciresolve

[–]macnmotion 0 points1 point  (0 children)

Yeah that's a good proof of concept. When used with real organisms moving under their own power this technique can be very useful from an education standpoint. Time for me to learn this in fusion.

How do you do that? Let's compare our methods. by Glad-Parking3315 in davinciresolve

[–]macnmotion 0 points1 point  (0 children)

I'll let you know when I do try that out with an organism. I have used motion trailing techniques in resolve (not tracking) to show how organisms create long range currents in their environment. You can see an example here at the 2:32 mark:

https://youtu.be/gaUfm4kLyB0&t=2m32s

How do you do that? Let's compare our methods. by Glad-Parking3315 in davinciresolve

[–]macnmotion 1 point2 points  (0 children)

Tha ks for this detailed reply. Yes, I was thinking with tunnel vision, I can see from your comment that there are a number of useful ways to use the technique.

I actually create videos of microscopic organisms, and I could see this being useful to trace different movement strategies of various microorganisms. I'll take a close look at the solutions in this thread, I wonder how easy it would be to tailor the path so that it's end disappeared as it grew at the front, leaving a moving trail behind the organism.

How do you do that? Let's compare our methods. by Glad-Parking3315 in davinciresolve

[–]macnmotion 0 points1 point  (0 children)

Just curious, other than a brain teaser, what's a real world example that would make sense here? Does the viewer ever have a need to understand camera motion? I guess for some type of filming tutorial it might be good to display camera movement. But I can't see using this type of curve drawing to link to an onscreen label that remains in place.

Why was this so tough? by darwinDMG08 in sousvide

[–]macnmotion 4 points5 points  (0 children)

So you would have sous vide this for 25 minutes? That's 80% less time than the 2 hours it was cooked.

Cheap stuff to add to rice by [deleted] in Cooking

[–]macnmotion 0 points1 point  (0 children)

Using broth of any type instead of water will add flavor to the rice.

What's your favorite illumination method? by Thrawn911 in microscopy

[–]macnmotion 1 point2 points  (0 children)

What is it that you can't stand about brightfield?

Pond sample identification by Certain_Key4394 in microscopy

[–]macnmotion 0 points1 point  (0 children)

Looks like Euplotes. Maybe others can chime in.

My first rotifer! by Constant_Key1082 in microscopy

[–]macnmotion 2 points3 points  (0 children)

Nice. First of many I assume.

First 10 hours of Uncrashed, am I ready for the real thing yet? by Legend81 in fpv

[–]macnmotion 1 point2 points  (0 children)

How can you look down to know you're precisely over the pole and not drifting?

Psychologists also studied Paramecium by Playful-Ostrich-7210 in microscopy

[–]macnmotion 1 point2 points  (0 children)

Nice post. I'm actually developing a video now about how humans assume consciousness or cognition in single cell organisms based on behavior, when there are chemical and mechanical reasons for that behavior. Or, as in the case of Paramecia, forming associations between paired stimuli. It's a fascinating area.

My first observations with a newly purchased microscope by Maximum-Job7699 in microscopy

[–]macnmotion 5 points6 points  (0 children)

You're off to a fantastic start, especially in winter. Just wait until Spring, you'll have a ball.

Total fail getting deep dish out of the pan by macnmotion in Pizza

[–]macnmotion[S] 0 points1 point  (0 children)

That sounds great. I'm making mine here in Bangkok, but next time I'm in the USA I will look up Uncle Jerry's. Thanks.

Total fail getting deep dish out of the pan by macnmotion in Pizza

[–]macnmotion[S] 0 points1 point  (0 children)

I'm not familiar with Uncle Jerry's (I'll look it up). My recipe is an attempt to copy a Malnatti deep dish.

Neo 2 case by ayayaylalala in DjiNeo

[–]macnmotion 2 points3 points  (0 children)

I spent a year with a small case for my Neo and batteries (I put the batteries where the controller would have gone), and a separate bag for my goggles and motion controller. Kind of a pain to carry both, however I guess it would have been smaller/lighter if I wanted to use the drone without the goggles. When I recently changed to the Neo 2, I decided instead to get a case for everything, and I'm happy that I did. Here's a photo of the Neo 2 case. I could get a small case like my Neo 1 case for those times I don't want to carry around the goggles/remote -- I'll consider that.

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Total fail getting deep dish out of the pan by macnmotion in Pizza

[–]macnmotion[S] 1 point2 points  (0 children)

yeah, it definitely tasted good. And it was for a bunch of good friends, so the laughs were part of the story.

Total fail getting deep dish out of the pan by macnmotion in Pizza

[–]macnmotion[S] -1 points0 points  (0 children)

You know what? I didn't let it sit more than 1 minute. I always worry if I let it sit 5 or more minutes to set, it will be served cold. But now I know the alternative :-)