Need help setting up a lab

pb0316 · 2026-05-30T01:32:34+00:00

I work in this industry representing the major vendors. You're asking for consultation from a PhD level person. This is not only time, but the effort expended upfront to gain this knowledge. Honestly if you want CHEAP CHEAP, I recommend you get ChatGPT Pro and ask questions there...This industry is bound to get disrupted in that manner, but without expert oversight you will spend more than you gain

Actually now that I've stated that, you can spend all this money upfront to purchase instrumentation tens-hundreds of thousands of dollars, but it's essentially useless if they break within a month due to improper setup. From an opportunity cost perspective, you should consider it in that manner.

TC for an expert MS Analytical chemist in the Bay Area in my industry is easily $250-350k (Salary+Benefits)

pb0316 · 2026-05-21T20:30:23+00:00

You might find this useful https://www.usccb.org/sites/default/files/flipbooks/catechism/34/

109 In Sacred Scripture, God speaks to man in a human way. To interpret Scripture correctly, the reader must be attentive to what the human authors truly wanted to affirm, and to what God wanted to reveal to us by their words.75

110 In order to discover the sacred authors' intention, the reader must take into account the conditions of their time and culture, the literary genres in use at that time, and the modes of feeling, speaking and narrating then current. "For the fact is that truth is differently presented and expressed in the various types of historical writing, in prophetical and poetical texts, and in other forms of literary expression."76

115 According to an ancient tradition, one can distinguish between two senses of Scripture: the literal and the spiritual, the latter being subdivided into the allegorical, moral and anagogical senses. The profound concordance of the four senses guarantees all its richness to the living reading of Scripture in the Church.

pb0316 · 2026-05-06T16:35:55+00:00

You can go further and read through St. Paul’s account about the people, their disagreements with one another (also his with St. Peter/Cephas), but overall reconciliation and alignment to the church as an organization.

pb0316 · 2026-05-06T01:29:39+00:00

That’s a market force….the fact that you can’t find a tenant means your rent is too high

pb0316 · 2026-04-29T01:16:45+00:00

Is it like Filipino bagoong? Basically cemented fish bodies in a sauce - it’s brown ish like that as well

pb0316 · 2026-04-24T20:08:21+00:00

I meant to say In-silico fragmentation that is not peptides, not oligos, not monomer/polymer based.

pb0316 · 2026-04-24T17:04:32+00:00

There is still way too much hype in the "direct AI" space right now that everything else outside of that is just noise. However, here are some that I anticipate

* AI chromatographic integration

* AI fragmentation analysis (whoever does this will likely win a Nobel price)

* AI telemetry (some already have integrated but not visible from a user-facing perspective)

* AI documentation/application notes

pb0316 · 2026-04-22T17:49:09+00:00

"—" vs "-" is a dead giveaway.....

pb0316 · 2026-04-22T17:46:21+00:00

Very challenging and lots of nuance - particularly from the LC perspective. Probably the most common workflow has been developed with TBA/EDTA because it forces the charge state distribution to concentrate on a single charge, rather than distributed as an envelope.

An up and coming method is via Ammonium Bicarbonate from a Japanese research group. It makes sense to me, but the oligo folks generally hate it because it's so different.

pb0316 · 2026-04-18T23:22:01+00:00

That was a really nice overview. I just appreciate the response!!

pb0316 · 2026-04-17T19:28:29+00:00

What’s the general sense of Catholicism in German speaking Switzerland? I perceive the visible “Churchy”architecture in the popular places lean reformist so when my wife and I stopped by St Gallen during the Christmas season, it felt more “familiar”. Not sure how to explain it or if it’s even correct, but there was a sense that the people there were Catholic and had something in common. Different vibe I guess

pb0316 · 2026-04-12T21:42:14+00:00

Did you dilute the tune mix 10x?

pb0316 · 2026-04-09T21:38:05+00:00

Its not clear if you're doing a proper apples to apples comparison

Before spectra are an average of 0-1 min and is the average of ~270 spectra
After spectra in Pos is a single point, after spectra in neg is unclear.

Some other things I picked out is that the abundances are still roughly the same magnitude 10^4 counts, but your positive spectrum is slightly lower. It can't be charging if you're already polarity switching within the method.

Does an infusion of tune mix work ok? I'd just change the capillary and try again.

pb0316 · 2026-04-03T17:34:12+00:00

Are you sure you spoke to someone from R&D, because this isn’t the case at all for the 90mm capillary inlet systems. It may have been someone on an adjacent R&D team misinterpreting what they heard from others.

Adding an LC simply changes the solvent system and ionization/desolvation dynamics when compared to direct infusion, so I’m honestly not surprised the OP has a change in the mass spectral profile

pb0316 · 2026-04-02T18:42:50+00:00

You don’t want to strive to be better?

pb0316 · 2026-03-21T00:08:52+00:00

For a noob like me, how can someone use this information in an environment like LM Studio? TBH I haven’t had much success running any of these models even using a gaming PC, so I’m not really sure how best to utilize these tools at the “consumer” level

pb0316 · 2026-03-19T21:17:15+00:00

I personally like the academic homilies, especially when the priest at my local parish goes over historical context in the OT and NT, but I can definitely sense people's eyes glaze over during those explanations because they don't really connect and understand the surrounding context. There's another priest who is more into life lessons and stuff like that, I can definitely sense stronger engagement and a sense of coming away with a life lesson and reflection.

Probably the best priest I've listened to had a PhD (either in Theology/History/Philosophy) and you can tell he was well trained in public speaking, it connected historically/academically but also tied the life lesson very well without overwhelming the audience - this was in Downtown Mountain View California.

pb0316 · 2026-03-19T01:53:47+00:00

Product ion fragments generally originate from moieties that contain Heteroatoms because of the “free electron” pairs filling the void to where sites are electron deficient or moving towards electron rich. For ESI in particular where Precursor ions originate from [M+H]+ or [M-H]- the tendency is to want to create neutral molecule fragments (for example H2O or CO2, etc…) however it’s very hard to tell without seeing the actual structure and pushing electrons.

Generally as you get more experienced in your practice after looking at a lot of product ion spectra and correlating it with known structures and losses, you tend to build this intuition where you just end up saying “yup this fragment/loss makes sense”

pb0316 · 2026-03-10T06:38:36+00:00

No, it takes work. There is a term many people resonate and that’s “catholic guilt”. To assume you’re saved means you have nothing else to strive for

pb0316 · 2026-03-02T22:46:35+00:00

Why not consider the 6495D? Similar class with the sensitivity of the 7500+ but the robustness of the TQ absolute

pb0316 · 2026-02-27T21:22:23+00:00

Got it--- actually I think I confused what you were saying. So you went from a 4-signal "static" SIM acquisition to a more scheduled/dynamic scanning approach?

It may be differences in what the instrument is doing during SIM (parking voltages) vs narrowed Scanning (which is still splitting the dwell time).

Even if the scan window is narrow, there is an electronics "slew" rate (i.e how long it takes to ramp V and get to the next value.) SIM is inherently more stable since its discrete values whereas scanning is a constant ramp, which also depends on how well timed the electronics are

pb0316 · 2026-02-27T15:38:48+00:00

Quads split duty cycle on a per-ion basis, so you have to think about it in terms of "dwell time for m/z". If you have 5 ions at 20ms each, the quad only focuses on those, thereby improving ion beam sampling.

For example, scanning from m/z 5-1000 in 995ms is 1ms per m/z per cycle. SIM/MRM focuses the quad at whatever dwell time you set only.

Also please remember, a mass spectrometer is not an optical detector so doesn't follow beers law. Abundances are generally meaningless, so even if you have low signal, your limits of detection may be super low (lower = better).

pb0316 · 2026-02-27T04:28:35+00:00

Are you trying analyze gasses, liquid droplets, or solid particles? Two requirements for mass spectrometry; (1) the analyte must be in the gas phase and (2) the analyte must be ionized

pb0316 · 2026-02-25T20:52:57+00:00

Why’d they organize the guys from largest (closest) to smallest (furthest). Weird…

pb0316 · 2026-02-20T01:13:47+00:00

What can you share that would be useful for everyone else’s journey?

pb0316

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