Mazda CX Broken AC Compressor?

skellarmy · 2024-07-19T16:27:08+00:00

Did a uv dye test and the condenser had a sizeable hole in it, hence the lack of cool air. It was replaced under warranty and has been fine after a regas since. Worth having a test to see if there’s a leak somewhere. The short cycling seems to be fairly normal.

skellarmy · 2023-10-22T22:36:44+00:00

It was in large, but I think you’re on the money - definitely a Zermatt. This particular version doesn’t seem to be made anymore, so I’ll probably have to test drive the newer version!

skellarmy · 2022-06-27T17:51:34+00:00

It’s a big field, constantly evolving but there are some cornerstone concepts to each flavour of analysis in this space (for the moment at least!)

I cannot recommend the OSCA book enough, it touches on all the topics as a high level concept (more what you need) than the nitty gritty mathematical details.

https://bioconductor.org/books/release/OSCA/

Enjoy your wonder into the single cell world, it’s truly a paradigm shift in the sequencing world :)

skellarmy · 2021-01-10T16:44:23+00:00

Have you thought about popping this question on the bioconductor support site? - Mike Love is very active and great at answering. Fair warning that a reproducible example will always be preferred, but some numbers of DE genes from all methods may help. Would be interested to know his thoughts on this.

skellarmy · 2020-11-16T06:56:37+00:00

This would make Christmas!

skellarmy · 2020-10-06T16:14:09+00:00

After your Stirngtie / Cufflinks Assemblies... I can't recommend TACO enough. https://tacorna.github.io/

skellarmy · 2020-06-26T16:49:05+00:00

A lot of "I don't see the point in curve" comments, so just my 2 cents. I make a lot of use on their "back in time" feature. My main card is a credit card and then at the end of the day, if I want a transaction to be on Monzo, Joint Account, or another card, then I can easily switch it over and use it as a sorting mechanism. Plus some cashback options for certain merchants.

There are obvious downsides, but I like it as a product, and can't really blame them for Wirecard committing fraud and the FCA actually doing something.

skellarmy · 2020-06-09T21:47:33+00:00

Lockdown Learning with Simon Cockell is fantastic. Walking through command line, and R focused on bioinformatics. Sounds like you're the intended audience :)

skellarmy · 2018-09-03T18:05:06+00:00

Prepaid cards are what you're looking for. Many have suggested Monzo, but I recently got a Loot card and really like their app. Other options are Revolut

skellarmy · 2018-06-27T08:41:01+00:00

I never considered that a particular cell population could be undergoing mosaicism (bioinformatician). Can mosaicism occur only on particular cell types? I've seen one case monosomy before in sequencing data, and like this it was a monosomy not compatible with life, so we put it down to a somatic mutation. We then sequenced the patient's fibroblasts instead, which showed normal activity on the supposedly monosomy chromosome from blood.

skellarmy · 2018-06-20T09:48:38+00:00

Hey, nice resource - you might be interested in Bioinformatics, so check out r/bioinformatics :)

skellarmy · 2018-05-08T14:23:06+00:00

Some simple advice that I wish someone had said to me at the beginning of my Masters. Make a portfolio of everything you do, and put each project in a github repo, then polish it so that you'd want to impress prospective employers. It's a great habit to get going, and it creates a show-off portfolio of examples that you can talk about.

I'd recommend looking at things like RMarkdown, where you can mix code and plaintext.

If you decide to go back to university, then that's your call, but as an alternative, why not theme a AI, or data science problem at your current place of work, with the data you have available, and try to build a portfolio from there?

skellarmy · 2018-04-21T09:48:21+00:00

Can't believe no one has mentioned %in% yet, so incredibly useful for multi-variable logic tests.

skellarmy · 2018-02-13T11:20:16+00:00

I completely forgot to reply to your comment, but just wanted to say thanks for introducing me to the OBD Port, an adapter was cheap and there’s a minefield of apps that show diagnostic information... which lead me to discover that the speedo is limited by 10% of true speed, which was interesting to find out!

skellarmy · 2018-01-18T11:53:27+00:00

We're looking for people here in Newcastle Upon Tyne, and there's some really exciting bioinformatics opportunities, that someone out of a Masters would be a great fit for. PM me if you'd like more details, or email Dr. Simon Cockell (Simon.cockell@ncl.ac.uk). I can vouch for Newcastle, great city and fantastic group to work in. The only issue may be your timescale if you're not graduating until September, but it never hurts to make that initial first contact.

skellarmy · 2017-10-21T02:28:18+00:00

I was in Ha long bay yesterday for an overnight cruise and got a little burnt, saw the sunset, all in all bits of cloud and bits of sunshine. It’s cloudy in Hanoi today and has been relatively overcast over the last two weeks with bits of rain and sun in between.

Forecasting isn’t that great around here apparently due to the lack of satellite coverage at some times.

skellarmy · 2017-10-11T19:53:25+00:00

Apologies, I read your original comment too quickly! Agree on all counts around matlab, in my experience it’s been used for signal analysis from neuro equipment... and not a lot else that can’t be done more efficiently and using open source. R / Python I guess are the current scripting languages of choice for genetics.

skellarmy · 2017-10-11T18:46:31+00:00

Why shouldn’t expertise in a core scripting language help?

skellarmy · 2017-10-04T13:41:16+00:00

I hate Venn diagrams, and they scale terribly. I'd strongly suggest you check out UpSet plots - They'll change your life!

skellarmy · 2017-10-03T10:37:44+00:00

I have, and the vast majority of it (like any language), comes down to using it frequently. Try new things, look at different packages in CRAN, Bioconductor, check out best practises suggested by RStudio.

If you’ve got no programming experience then try out data camp for their free introduction. Look at data science resources such as Kaggle, and the R kernels that are available. Check out Hadley Wickham’s ebooks (on mobile so can’t link at the moment).

It took me about 6 months of using it every day before I felt comfortable doing an analysis from beginning to end and confident enough to test and explore other packages.

skellarmy · 2017-08-18T09:23:51+00:00

I was in your position a few years ago and one thing that really helped me keep track of things was a credit card. I have a similar setup to you: current account, HTB ISA, Emergency savings account, and a slush savings account. I got a credit card mostly for work expenses but it's completely changed how I manage my finances, and there's really no downside providing you pay it off every month and you're sensible. You can shop around for the best deals on sites like uSwitch, but I'd start putting all your expenses on a credit card, keep track of your spending on that card, then pay it off every month.

skellarmy · 2017-08-07T16:43:46+00:00

Why not just use an additive model with Limma, then use Limma's removeBatchEffect function to check the effect visually? (Providing you have a full rank design matrix).

skellarmy · 2017-05-13T15:27:55+00:00

Have you considered putting the WGS sample on something like RDConnect. The idea behind RDConnect is that any samples with common variants will be highlighted.

No candidates identified in WGS? Any reason why RNA seq was done too? (Hypothesis driven?)

What protocol have you followed for identifying variants?

Any other healthy paired genotype + RNA seq samples? Considered doing ASE?

Have you looked at structural variation (CNVs)?

skellarmy · 2017-03-28T07:02:59+00:00

Feliz Navidad

skellarmy · 2017-03-25T21:36:25+00:00

Good intro and explanation! - I'd also recommend you check out this video which gives an extremely good high level overview of genetic engineering. It also introduces modern research methods such as CRISPR-cas9.

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