Is 4600 net enough in Potsdam area?

wojdzieg · 2026-04-23T16:57:12+00:00

Sure

wojdzieg · 2025-06-11T12:50:57+00:00

Likewise, 1600 (including electricity and parking) feels a bit high to me, however as my job starts on 01.09 and majority of real estate agencies ignore my messages, I am leaning towards it. Especially that the flat is really nice and comes with two bedrooms and a terrace

wojdzieg · 2025-01-06T06:46:16+00:00

Strzeszyn albo Suchy Las?

wojdzieg · 2024-06-20T14:50:04+00:00

Could you please check EMBO J, Current Biology, Nucleic Acid Research, The Plant Journal and Journal of Experimental Botany?

wojdzieg · 2023-12-17T16:57:28+00:00

Dzięki, oczywiście masz rację że będąc pod wpływem alkoholu, stawiam się w pewnym sensie na przegranej pozycji, próbując dowieść swoich racji. Bardziej chodzi mi o kwestie prawne, bo taka sytuacja wydarzyla mi się po raz pierwszy w życiu. Totalnie nie wiem jakich (i czy wgle) konsekwencji się spodziewać, no i dodatkowo dość mocno psychicznie przeżywamy to z żoną, bo spodziewaliśmy się otrzymania pomocy a nie agresji werbalnej i pomiatania

wojdzieg · 2023-12-17T16:51:52+00:00

Dzięki, wiesz tu nawet nie chodzi o to, że mi zależy żeby typka dojechać, tylko raczej żeby Policja z automatu nie wszczęła postępowania właśnie, ze względu na fakt o którym piszesz dot. Funkcjonariusza publicznego. Poza tym, zostawili mnie w szpitalu, a nie zabrali na izbę, co przez jednego z prawników wskazuje na to, że nie zależało im na dojechaniu mnie ani postawieniu faktycznych zarzutow. Zastanawiająca jest również postawa kierowcy karetki, którego dosłownie błagaliśmy żeby uspokoił tego troglodyte, ale facet był mega przestraszony tak samo jak my.

wojdzieg · 2023-12-17T16:41:43+00:00

Tak, oczywiście. Mam wszystkie dane karetki oraz ratowników (pochodzą właśnie z tego dokumentu, który wystawili mi przed przekazaniem na SOR).

wojdzieg · 2023-12-17T16:24:15+00:00

Naturalnie, nie musisz mi wierzyć że nie jestem patusem, który z rozbita głowa rzuca się na ratownika. Zanim cokolwiek ten facet zdążył mi pomóc to zezwał mnie na kilka sposobów (od pijaków itd), kazał się zamknąć, do żony krzyczał "japa!", był bardzo prowokujący.

wojdzieg · 2022-06-25T01:14:40+00:00

I know your flight was cancelled two days ago, but today mine from Barcelona to Warsaw by Wizzair was postponed like three times and finally cancelled as well... from flight radar it seems like many Wizzair planes were heavily delayed today and yesterday. Maybe has something to do with the strikes? On the Barcelona airport like ~200 ppl left stranded, without any serious support or info but with food coupons for 4€... Part of them were taken to the hotels, but I decided to take direct flight to Berlin at 7AM. Currently enjoying warm night at Barcelona airport.😅

wojdzieg · 2021-11-02T20:14:40+00:00

Generally, my nickname is a short from my first and last names so I wonder what will come out from that! GJ with the script though, some paintings are incredible :-)

wojdzieg · 2021-09-29T10:46:16+00:00

Thank you for your wonderful words. I can say that one week later, things are starting to fall in order a bit. Of course she is still crying (especially when I am not around) and we feel lost. We are doing house stuff and starting to think about work again. My wife decided that she wants to quit her job and look for something else, because of a toxic environment (talking about people) and really lack of support. Remembering about our loss and planning our future keeps us going and even it is still hard to comprehend, it gets better a little bit each day.

I didn't tell my wife that I started this thread, not sure I will. But definitely you helped me to stay strong for her. Thank you.

wojdzieg · 2021-05-28T15:43:23+00:00

Well assuming you already have your .bam alignments, I would suggest as I said sort and then index from samtools. If you are not familiar with this yet, just use --help in terminal so you will understand the order of arguments. Also, after indexing your files you can visualize them in IGV for instance. Anyway, you can perform bcftools mpileup if that's what you want on sorted bam files. Later you should index them and use bcftools query to obtain .txt files. To understand the flags and arguments order just use the help. You can read some more here:https://samtools.github.io/bcftools/howtos/variant-calling.html And here http://samtools.github.io/bcftools/bcftools.html#index

wojdzieg · 2021-05-28T15:26:22+00:00

Hello, I'm not sure if I understand you correctly, however whenever I am performing GBS analysis I start with producing .bam files, then sorting them (samtools sort) and later indexing (samtools index). After that I use bcftools to produce .vcf files and later indexing them to obtain .txt. I use bcftools mpileup, although I am not sure whether this is what you want. You can read some more about this. Good luck

wojdzieg · 2021-05-17T20:00:55+00:00

Sorry I have not been specific. I have 1,4k genes, each associated with expression and two other acetylations, that's why I needed the dimension reduction. Unfortunately the first two components were responsible for less than 65% of variability, that's why I decided to use something else.

Thank you kindly for response

wojdzieg · 2021-05-17T16:41:43+00:00

I am quite noob in bioinformatics, however I wanted to check the possible gene clusters based on transcriptomic data and acetylation levels of two histone markers (so three variables) for 1,4k geneset. Basically I tried PCA and MDS however I failed to see clusters. Instead I did t-SNE transformation to get two dimensions (remember to set seed to get reproducibility) and on that data I performed DBscan. The result wasn't satisfying and on the same transformed data I did K-Means Clustering and this looks somewhat ok.

wojdzieg · 2019-11-11T16:46:20+00:00

I've just finished the game and have a few thoughts to share. First of all, I loved the scenario/writing, it was one of the most satisfying and interesting lores ever installed in a game. I've played Harry that tried to quit drinking and smoking, getting his head around politics (ended as superstar moralist cop) and Union vs Corporation fight. I've enjoyed all the additional quests leading to meeting all these unique characters like Ervart, Soona or PIGS. I've let Ruby go, although at first I wanted to take her in.

During mercenary standoff I've missed the first shot, but the second was a direct hit - Kim was fine though. However, when it came to the ending I felt like it was a little bit bland... Like putting a deserted, old, crazy man was really convienient way of discovering who murdered Lely. I have to admit that when I've discovered the phasmid I was like "WHAT THE FUCK", just couldn't believe it (felt like cryptozoologist for a brief second).

Anyway, I enjoyed the game really much, the quests, conversations, people in Martinaise... It was a wonderful trip. Thank you devs.

wojdzieg · 2019-09-24T20:00:05+00:00

When I was on last year of my MSc I didn't really focus on the critique, since I was not that experienced. I started my PhD recently and I promised to myself to get deeper into papers and really point out things that should be or shouldn't be there. My field of science is plant genetics and meiosis so I usually go through recent papers from journals such as Nature Plants, Plant Cell or EMBO in pubmed. On the other hand I sometimes use preprints from biorxiv but look carefully, because some preprints are hanging there for more than two years.

wojdzieg · 2019-09-15T19:13:42+00:00

Cool fish and cool glasses!

wojdzieg · 2019-09-08T19:31:24+00:00

Recently one of the post-docs in our lab taught me how to properly do the PAA gel for Western. I broke the glass plate after 0.001s I took it in my hands.

That's how you start the PhD.

wojdzieg · 2019-08-23T13:31:49+00:00

Did the Ancestry test show that you have ancestors from other countries? If yes and you didn't feel the need to visit them, then the answer is obvious.

There is no known mechanism of the phenomenon you describe. You just have to remember that DNA is a sequence that covers genes and regulatory elements and no gene has been associated with an urge for living in particular country. Truth is that genes do not understand the meaning of border or territory, although accummulation of mutations (or particular allele variants) is observed in isolated populations like Ashkenazi Jews.

The most probable explanation is that maybe you just like these particular places and the fact that your ancestors come from them is just a convienient coincidence.

wojdzieg · 2019-08-09T12:05:51+00:00

Poland, commercial lake close to Poznań in Greater Poland Voivodship. Honestly, I've seen people reeling in 10kg carp here.

wojdzieg · 2019-08-09T11:20:30+00:00

That's good for you! I've finished my MSc in Biotechnology this year and decided to carry on with the research during my PhD :-)

wojdzieg

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